Results 241 to 250 of about 3,338,865 (284)
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Density gradient centrifugation in angle-head rotors
Analytical Biochemistry, 1964Abstract By utilizing DNA and T3 bacteriophage as test materials, good isopyenic banding in CsCl was obtained in #30 and #40 Spinco fixed-angle rotors. Values for the buoyant densities of the materials tested agree with published values and density gradients formed after prolonged centrifugation approximate the calculated compositional gradient at ...
W D, FISHER, G B, CLINE, N G, ANDERSON
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Differentiation of reoviruses and picornaviruses by density gradient centrifugation
Archiv f�r die gesamte Virusforschung, 1967The two HA moieties of reovirus (infectious particle and top component) were recentrifuged in a CsCl gradient. They were stable under these conditions and purified to such an extent that the top component HA moiety contained only 4 TCD50 per unit. Mixtures of poliovirus and reovirus (all three prototypes) were centrifuged in a CsCl density gradient ...
R, Engler, M, Taher, A, Fouad
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Density Gradient Centrifugation Studies on Rabies Virus
Journal of Bacteriology, 1966Neurath, A. Robert (The Wistar Institute, Philadelphia, Pa.), Tadeusz J. Wiktor, and Hilary Koprowski . Density gradient centrifugation studies on rabies virus. J. Bacteriol. 92: 102–106.
A R, Neurath, T J, Wiktor, H, Koprowski
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Density gradient centrifugations of adenovirus-antibody complexes
Archiv f�r die gesamte Virusforschung, 1965Buoyant density sedimentations in CsCl of adenovirus type 5 and virus-antibody complexes has been described. It has been demonstrated that virus which survives a certain dose of antibody is found at densities lower than that of untreated particles suggesting a coating of the virus with antibody.
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Isolation of Endocitic Organelles by Density Gradient Centrifugation
2008Advanced prefractionation strategies, in combination with highly sensitive and accurate mass spectrometers provide powerful means to detect and analyze low abundant proteins on the subcellular and organelle-specific level. Among enrichment techniques, subcellular fractionation has become the most commonly used.
Mariana Eça Guimarães, de Araùjo +2 more
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Isolation of bovine eosinophils by density gradient centrifugation
American Journal of Veterinary Research, 1985SUMMARY A method for the isolation of an enriched population (> 60%) of bovine eosinophils was developed, using density gradient centrifugation. Eosinophils were isolated from peripheral blood by centrifugation on a density gradient medium of sp gr 1.092.
W H, Chambers +3 more
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Photometric scanning of centrifuged density gradient columns
Analytical Biochemistry, 1963Abstract Results obtained with an apparatus for recording the ultraviolet absorbance at all depths in a centrifuged density gradient column and for collecting consecutive samples are presented. A heavy sucrose solution is forced from a motor-driven syringe into the bottom of a gradient column.
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The Isolation of Satellite DNA by Density Gradient Centrifugation
2003The term satellite DNA is used for a DNA component that gives a sharp band in a density gradient and can be resolved from the broader main band of DNA in the gradient. The usual gradient material is CsCl in aqueous buffer and the Cs(+) ions form a density gradient in a centrifugal field. DNA in the solution sediments to its isopycnic point. The density
C A, Cooney, H R, Matthews
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The Optimization of Density Gradients for Zonal Centrifugation
Separation Science, 1969Abstract By using established equations and techniques, it is possible to compute sucrose gradients which are optimized to minimize volume expansion of a zone during sedimentation in zonal rotors. These gradients have been tested experimentally, and it has been found that the greatest expansion of the sample zone occurred during the early stage of ...
S. P. Spragg, R. S. Morrod, C. T. Rankin
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Density gradient centrifugation of a graft copolymer
Journal of Polymer Science Part A: General Papers, 1964AbstractA well‐defined graft copolymer of polystyrene and cellulose acetate has been investigated using the density gradient ultracentrifugation method. The initial sample showed two peaks. These were found to be pure graft copolymer and polystyrene; the latter was easily removed by further extraction of the mixture with benzene. The composition of the
H. A. Ende, V. Stannett
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