Results 1 to 10 of about 2,522 (141)

Pathogen-driven nucleotide overload triggers mitochondria-centered cell death in phagocytes. [PDF]

PLoS Pathogens, 2023
Staphylococcus aureus is a dangerous pathogen that evolved refined immuno-evasive strategies to antagonize host immune responses. This involves the biogenesis of death-effector deoxyribonucleosides, which kill infectious foci-penetrating macrophages ...
Nicoletta Schwermann, Rita Haller, Sebastian Koch, Guntram A Grassl, Volker Winstel   +4 more
doaj   +2 more sources

Characterisation of an Escherichia coli line that completely lacks ribonucleotide reduction yields insights into the evolution of parasitism and endosymbiosis [PDF]

eLife, 2023
Life requires ribonucleotide reduction for de novo synthesis of deoxyribonucleotides. As ribonucleotide reduction has on occasion been lost in parasites and endosymbionts, which are instead dependent on their host for deoxyribonucleotide synthesis, it ...
Samantha DM Arras, Nellie Sibaeva, Ryan J Catchpole, Nobuyuki Horinouchi, Dayong Si, Alannah M Rickerby, Kengo Deguchi, Makoto Hibi, Koichi Tanaka, Michiki Takeuchi, Jun Ogawa, Anthony M Poole   +11 more
doaj   +2 more sources

Response of Escherichia coli chemotaxis pathway to pyrimidine deoxyribonucleosides [PDF]

Microbiology Spectrum
Nucleosides are essential components of all living cells. Bacteria use salvage pathways to import nucleosides from their environment and to utilize them for nucleic acid biosynthesis, but also as alternative sources of carbon, nitrogen and energy. Motile
Malay Shah, Wenhao Xu, Victor Sourjik
doaj   +2 more sources

Targeting host deoxycytidine kinase mitigates Staphylococcus aureus abscess formation [PDF]

eLife
Host-directed therapy (HDT) is an emerging approach to overcome antimicrobial resistance in pathogenic microorganisms. Specifically, HDT targets host-encoded factors required for pathogen replication and survival without interfering with microbial growth
Volker Winstel, Evan R Abt, Thuc M Le, Caius G Radu   +3 more
doaj   +2 more sources

Staphylococcus aureus Multiplexes Death-Effector Deoxyribonucleosides to Neutralize Phagocytes

Frontiers in Immunology, 2022
Adenosine synthase A (AdsA) is a key virulence factor of Staphylococcus aureus, a dangerous microbe that causes fatal diseases in humans. Together with staphylococcal nuclease, AdsA generates deoxyadenosine (dAdo) from neutrophil extracellular DNA traps ...
Eshraq Tantawy, Eshraq Tantawy, Nicoletta Schwermann, Nicoletta Schwermann, Tjorven Ostermeier, Tjorven Ostermeier, Annette Garbe, Heike Bähre, Marius Vital, Volker Winstel, Volker Winstel   +10 more
doaj   +1 more source

Deoxyribonucleoside Phosphoramidites [PDF]

Current Protocols in Nucleic Acid Chemistry, 2001
AbstractThe detailed preparation of deoxyribonucleoside phosphoramidites bearing a 4‐[N‐methyl‐N‐(2,2,2‐trifluoroacetyl)amino]butyl group for P(III) protection is presented. The use of this group circumvents nucleobase alkylation during oligonucleotide deprotection. Two syntheses of phosphoramidites starting from either a phosphordichloridite precursor
Wilk, Andrzej, Grajkowski, Andrzej, Chmielewski, Marcin K., Beaucage, Serge L., Phillips, Lawrence R.   +4 more
openaire   +3 more sources

Studies on Transcriptional Incorporation of 5'-N-Triphosphates of 5'-Amino-5'-Deoxyribonucleosides. [PDF]

PLoS ONE, 2016
In this study, several RNA polymerases were used for the first time to examine the possibility of transcriptional incorporation of 5'-N-triphosphates of 5'-amino-5'-deoxyribonucleosides (5'NH NTPs).
Weronika Kotkowiak, Anna Pasternak, Ryszard Kierzek   +2 more
doaj   +1 more source

Cloning and expression of recombinant purine nucleoside phosphorylase in the methylotrophic yeast Pichia pastoris

Journal of Advanced Biotechnology and Experimental Therapeutics, 2023
Purine Nucleoside Phosphorylase (PNP) is an enzyme involved in biosynthetic pathway of purine nucleosides. Purine nucleoside phosphorylase catalyzes the cleavage of the glycosidic bond of ribo- or deoxyribonucleosides to form the purine base and ...
Jaloliddin Abdurakhmanov, Sobirdjan Sasmakov, Shukhrat Khasanov, Oybek Ashirov, Farkhod Eshboev, Khayotjon Dolimov, Saodat Gaynazarova, Tulkin Sadullaev, Oygul Yarilkaganova, Khusan Nasriddinov, Asadali Baymirzaev, Artyom Makhnyov, Shakhnoz Azimova   +12 more
doaj   +1 more source

Conventional Raman, SERS and TERS Studies of DNA Compounds

Chemosensors, 2023
DNA identification is possible by detecting its components through vibrational spectroscopy. Conventional Raman, Surface-enhanced Raman spectroscopy (SERS) and Tip-enhanced Raman spectroscopy (TERS) have shown a high capacity for the exploration of ...
Wafa Safar, Aicha Azziz, Mathieu Edely, Marc Lamy de la Chapelle   +3 more
doaj   +1 more source

Incorporation characteristics of exogenous 15N-labeled thymidine, deoxyadenosine, deoxyguanosine and deoxycytidine into bacterial DNA.

PLoS ONE, 2020
Bacterial production has been often estimated from DNA synthesis rates by using tritium-labeled thymidine. Some bacteria species cannot incorporate extracellular thymidine into their DNA, suggesting their biomass production might be overlooked when using
Kenji Tsuchiya, Tomoharu Sano, Noriko Tomioka, Ayato Kohzu, Kazuhiro Komatsu, Ryuichiro Shinohara, Shinji Shimode, Tatsuki Toda, Akio Imai   +8 more
doaj   +1 more source