Results 61 to 70 of about 4,589,695 (260)

Facile expression of proteins with desired N-terminal amino acid via an engineered cysteine protease domain

Communications Biology
The removal of N-terminal methionine (Met) or fused N-terminal purification tags to expose the first amino acid of the target protein is critically important for studying the potential regulatory role of the N-terminal residue.
Xiangman Zou, Zhi Liu, Fengnan Song, Wei Zhou, Jiaying Hang, Chenchen Feng, Tianhong Yuan, Jinhua Dong, Wei Shi, Feng Tang, Wei Huang   +10 more
doaj   +1 more source

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Wie können Biologielehramtsstudierende auf fachdidaktische Reflexionen über selbsterteilte Unterrichtsphasen vorbereitet werden?

Zeitschrift für Didaktik der Biologie - Biologie Lehren und Lernen
Im Jahr 2017/18 wurde an der Universität Bremen ein Aufgabenkonzept für Biologielehramtsstudierende entwickelt und evaluiert, das gezielt die fachspezifische Reflexionsfähigkeit in Schulpraxisphasen fördern soll.
Lisa Jiang, Dörte Ostersehlt
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Towards a Design-Based Approach to Accounting Research

SSRN Electronic Journal, 2022
Armstrong et al. (2022) review the empirical methods used in the accounting literature to draw causal inferences. They document a growing number of studies using quasi-experimental methods and provide a critical perspective on this trend as well as the use of these methods in the accounting literature.
openaire   +3 more sources

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Innovativ formidling af førsteårsstuderende som et design-based research-forløb

Læring og Medier, 2016
14 videoproduktioner, 4 tegneserier, 3 filmfortællinger, 2 sange, 2 nyhedsindslag, 2 børnebøger, 2 facebook-sider, 1 novelle og 1 toiletrulle var blandt resultaterne, da 112 førsteårsstuderende i det obligatoriske kursus Astrofysik på fysikuddannelsen på
Ole Eggers Bjælde, Rasmus Brøgger Najbjerg   +1 more
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Integrating Design Thinking and Problem-Based Learning in MOOCs

Journal of Open, Flexible and Distance Learning
This article presents our findings concerning a MOOC named ’Introduction to R programming language’, in which we applied design thinking combined with problem-based learning to enhance student engagement and improve the learning experience.
João Alberto Arantes do Amaral, Izabel Patricia Meister, Alessandro dos Santos Faria, Felipe Mancini, Valeria Sperduti Lima, Luciano Gamez   +5 more
doaj   +1 more source