Results 51 to 60 of about 219,126 (267)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Characterization of the materials of the ceiling that covers the Mayor Sacristhi of Seville's Cathedral

Materiales de Construccion, 2004
In this article we expose the technics used to characterize the materials of the ceiling that covers the Mayor sacristy of Seville's Cathedral and the causes of the loosening of mortar and stone fragments, we also expose some ways to overcome this fact.
J. Barrios, A. Barrios, C. Rodríguez
doaj   +1 more source

ALDOA Promotes Glycolysis and NLRP3/GSDMD Pyroptosis to Accelerate ALS Progression

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Amyotrophic lateral sclerosis (ALS) is characterized by progressive motor neuron degeneration. Glycolytic dysregulation is implicated in disease progression, yet the underlying mechanisms remain unclear. This study investigates how Aldolase A (ALDOA) drives ALS progression through glycolysis‐mediated motor neuron pyroptosis.
Kaixin Yan, Yan Jiang, Yuxuan Yong, Tianshuo Zhang, Niannian Zhang, Qianqian Zeng, Xue Gong, Li Meng, Fangfang Bi, Yongmin Liu   +9 more
wiley   +1 more source

Facilitated core-edge integration through divertor nitrogen seeding in the HL-2A tokamak

Nuclear Fusion
A divertor detachment with sustainable high-performance plasmas has been achieved through divertor nitrogen seeding in the HL-2A tokamak. The closed divertor structure facilitates achieving high divertor neutral pressure due to its efficient particle ...
N. Wu, J. Cheng, K.Y. Yi, R. Wang, M.K. Han, Z.H. Huang, W.C. Wang, Y. He, L.W. Yan, H.L. Du, J.M. Gao, H.C. Ma, X.X. He, Z.C. Yang, J. Wen, K. Zhang, T. Wu, W.P. Guo, X. Yu, L. Liu, Y.G. Li, L. Nie, Z.B. Shi, D.Z. Wang, W.L. Zhong   +24 more
doaj   +1 more source

Digital Cognitive Phenotyping for Differential Diagnosis and Monitoring in Neurological Conditions

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To assess the utility, accessibility, and equivalence to supervised scales of online cognitive assessment in older individuals with cognitive impairment. Methods Patients with Alzheimer's disease (AD, n = 31), idiopathic normal pressure hydrocephalus (iNPH, n = 26), and traumatic brain injury (TBI, n = 23) completed online cognitive ...
Martina Del Giovane, Valentina Giunchiglia, Michael C. B. David, Magdalena A. Kolanko, William R. Trender, Peter J. Hellyer, Harmeena Kaur, David J. Sharp, Christopher Carswell, Paresh A. Malhotra, Adam Hampshire   +10 more
wiley   +1 more source

Integrated PANoptosis Profiling Identifies Immunosuppressive Subtypes and a Prognostic Signature With Functional Validation of MLKL in Glioblastoma

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective The prognosis of glioblastoma (GBM) remains highly unfavorable, largely due to high tumor heterogeneity and an immunosuppressive microenvironment. However, the functional role of PANoptosis in this context is poorly understood. Methods Patients were stratified via K‐means clustering. A risk score model was constructed using prognosis‐
Langfei Tian, Minghui Zhao, Yuanbo Hu, Kaiyue Wang, Haiguang Liu, Zetong Bai, Kebin Zheng   +6 more
wiley   +1 more source

The Association of Physical Function With Psychosocial Patient‐Reported Outcomes in People With Systemic Lupus Erythematosus

Arthritis Care &Research, EarlyView.
Objective To evaluate how modifiable psychosocial factors and fatigue relate to physical functioning in patients with systemic lupus erythematosus (SLE). Methods In this cross‐sectional study of two demographically distinct cohorts (Approaches to Positive, Patient‐Centered Experiences of Aging with Lupus [APPEAL] and California Lupus Epidemiology Study
Mrinalini Dey, Jessica Fitzpatrick, Maria Dall'Era, Charmayne Dunlop‐Thomas, Courtney Hoge, S. Sam Lim, Patricia P. Katz, Jinoos Yazdany, Laura C. Plantinga   +8 more
wiley   +1 more source

The core–edge integrated neon-seeded scenario in deuterium–tritium at JET

Nuclear Fusion
This paper reports the first experiment carried out in deuterium–tritium addressing the integration of a radiative divertor for heat-load control with good confinement. Neon seeding was carried out for the first time in a D–T plasma as part of the second
C. Giroud, I.S. Carvalho, S. Brezinsek, A. Huber, D. Keeling, J. Mailloux, R.A. Pitts, E. Lerche, R. Henriques, J. Hillesheim, K. Lawson, M. Marin, E. Pawelec, M. Sos, H.J. Sun, M. Tomes, S. Aleiferis, A. Bleasdale, M. Brix, A. Boboc, J. Bernardo, P. Carvalho, I. Coffey, S. Henderson, D.B. King, F. Rimini, M. Maslov, E. Alessi, T. Craciunescu, M. Fontana, J.M. Fontdecaba, L. Garzotti, Z. Ghani, L. Horvath, I. Jepu, J. Karhunen, D. Kos, E. Litherland-Smith, A. Meigs, S. Menmuir, R.B. Morales, S. Nowak, E. Peluso, T. Pereira, V. Parail, G. Petravich, G. Pucella, P. Puglia, D. Refy, S. Scully, M. Sertoli, S. Silburn, D. Taylor, B. Thomas, A. Tookey, Ž. Štancar, G. Szepesi, B. Viola, A. Widdowson, E. de la Luna, JET Contributors   +60 more
doaj   +1 more source

M & A as a possible market strategy [PDF]

Marketing (Beograd. 1991), 2005
In this paper it is shown a short list of terms and history of takeovers and mergers, as well as the types of structural transformation of companies.
Vasiljev Dejan S., Bašić Tamara
doaj