Research on the Threshold Determination Method of the Duffing Chaotic System Based on Improved Permutation Entropy and Poincaré Mapping. [PDF]
Zhou J, Li Y, Wang M.
europepmc +1 more source
Tau acetylation at K331 has limited impact on tau pathology in vivo
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source
New spectrophotometric method with KMnO4 for determination of hypochlorite in commercial bleaches
Hypochlorite with a strong germicidal and bleaching action forms the active component in most commercial bleaches. The new method, an instrument-based and simple experimental procedure involves the reaction of the hypochlorite with arsenious oxide (As2O3)
P. Gengan, S.B. Jonnalagadda
doaj
Rapid method for determination of antimicrobial susceptibilities pattern of urinary bacteria [PDF]
Method determines bacterial sensitivity to antimicrobial agents by measuring level of adenosine triphosphate remaining in the bacteria.
Barza, M. J. +5 more
core +1 more source
Calpain small subunit homodimerization is robust and calcium‐independent
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Validation of Free Formaldehyde Determination Method in DT Vaccine and Tetanus Toxoid Antigen. [PDF]
Zali S, Es-Haghi A, Ranjbar Rafie H.
europepmc +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Study on the mesh size determination method of blast wave numerical simulation with strong applicability. [PDF]
Kuang Z, Liu Z.
europepmc +1 more source
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel +13 more
wiley +1 more source

