Results 11 to 20 of about 40,426 (262)

The IQ-compete assay for measuring mitochondrial protein import efficiencies in living yeast cells. [PDF]

FEBS Lett
The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Hoffman Y, Egeler A, Rödl S, Herrmann JM.   +3 more
europepmc   +2 more sources

Association of Genetic Mutations inPlasmodium vivax dhfr with Resistance to Sulfadoxine-Pyrimethamine: Geographical and Clinical Correlates

Antimicrobial Agents and Chemotherapy, 2001
M. Imwong, Sasithon Pukrittakayamee, S. Looareesuwan, G. Pasvol, Jean Poirreiz, N. White, G. Snounou   +6 more
semanticscholar   +3 more sources

Selective Non-toxics Inhibitors Targeting DHFR for Tuberculosis and Cancer Therapy: Pharmacophore Generation and Molecular Dynamics Simulation

Bioinformatics and Biology Insights, 2023
Dihydrofolate reductase (DHFR) is a crucial enzyme that catalyzes the conversion of folic acid. Its reserved properties and significance in both human (h-DHFR) and mycobacterium (mt-DHFR) make it a challenging target for developing drugs against cancer ...
Ghyzlane EL Haddoumi, Mariam Mansouri, Houda Bendani, Mohammed Walid Chemao-Elfihri, Jouhaina Kourou, Hanane Abbou, Lahcen Belyamani, Ilham Kandoussi, Azeddine Ibrahimi   +8 more
doaj   +1 more source

Design, Synthesis, and Antimicrobial Evaluation of a New Series of N-Sulfonamide 2-Pyridones as Dual Inhibitors of DHPS and DHFR Enzymes

ACS Omega, 2020
Sulfonamides and trimethoprim (TMP) drugs are normally used to inhibit the action of dihydropteroate synthase (DHPS) and dihydrofolate reductase (DHFR) enzymes, respectively. In this work, a new series of N-sulfonamide 2-pyridone derivatives that combine
Rasha A. Azzam, Rasha E. Elsayed, G. Elgemeie   +2 more
semanticscholar   +1 more source

Histidine hydrogen-deuterium exchange mass spectrometry for probing the microenvironment of histidine residues in dihydrofolate reductase. [PDF]

PLoS ONE, 2011
Histidine Hydrogen-Deuterium Exchange Mass Spectrometry (His-HDX-MS) determines the HDX rates at the imidazole C(2)-hydrogen of histidine residues. This method provides not only the HDX rates but also the pK(a) values of histidine imidazole rings.
Masaru Miyagi, Qun Wan, Md Faiz Ahmad, Giridharan Gokulrangan, Sara E Tomechko, Brad Bennett, Chris Dealwis   +6 more
doaj   +1 more source

Functional expression of parasite drug targets and their human orthologs in yeast. [PDF]

PLoS Neglected Tropical Diseases, 2011
The exacting nutritional requirements and complicated life cycles of parasites mean that they are not always amenable to high-throughput drug screening using automated procedures. Therefore, we have engineered the yeast Saccharomyces cerevisiae to act as
Elizabeth Bilsland, Pınar Pir, Alex Gutteridge, Alexander Johns, Ross D King, Stephen G Oliver   +5 more
doaj   +1 more source

Identification of DHPS and DHFR of pythium insidiosum from the first Chinese skin and subcutaneous pythiosis

Informatics in Medicine Unlocked, 2023
Based on our previous studies, it is speculated that DHPS and DHFR are potential targets for the treatment of pythiosis. The Physicochemical properties, hydrophilicity/hydrophobicity, subcellular localization, signal peptide, secondary structure ...
Haiyan Zhang
doaj   +1 more source

Establishment of a Dihydrofolate Reductase Gene Knock-In Zebrafish Strain to Aid Preliminary Analysis of Congenital Heart Disease Mechanisms

Frontiers in Cardiovascular Medicine, 2021
Background: The dihydrofolate reductase (DHFR) gene is imperative in development, therefore it is essential to explore its effects on heart development. Thus, here a dhfr zebrafish knock-in (KI) strain was constructed.Methods: CRISPR/Cas9 technology was ...
Ke Gong, Ting Xie, Yifeng Yang, Yong Luo, Yun Deng, Yun Deng, Kun Chen, Kun Chen, Zhiping Tan, Zhiping Tan, Hui Guo, Li Xie   +11 more
doaj   +1 more source

A designed phenylalanyl-tRNA synthetase variant allows efficient in vivo incorporation of aryl ketone functionality into proteins [PDF]

, 2002
Incorporation of non-natural amino acids into proteins in vivo expands the scope of protein synthesis and design. p-Acetylphenylalanine was incorporated into recombinant dihydrofolate reductase (DHFR) in Escherichia coli via a computationally designed ...
Carrico, Isaac S., Datta, Deepshikha, Mayo, Stephen L., Tirrell, David A., Wang, Pin   +4 more
core   +2 more sources

Molecular determinants of sulfadoxine-pyrimethamine resistance in Plasmodium falciparum in Nigeria and the regional emergence of dhps 431V

International Journal for Parasitology: Drugs and Drug Resistance, 2016
There are few published reports of mutations in dihydropteroate synthetase (dhps) and dihydrofolate reductase (dhfr) genes in P. falciparum populations in Nigeria, but one previous study has recorded a novel dhps mutation at codon 431 among infections ...
Mary C. Oguike, Catherine O. Falade, Elvis Shu, Izehiuwa G. Enato, Ismaila Watila, Ebenezer S. Baba, Jane Bruce, Jayne Webster, Prudence Hamade, Sylvia Meek, Daniel Chandramohan, Colin J. Sutherland, David Warhurst, Cally Roper   +13 more
doaj   +1 more source