Results 61 to 70 of about 23,071 (215)
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source
Four recombinant antibodies (ABCD_RB877 to ABCD_RB880) detect by ELISA a purified peptide from the Dictyostelium discoideum DstD protein. ABCD_RB877 and ABCD_RB878 also recognize the endogenous DstD and the GFP-DstD protein stably expressed in D ...
Justine Toinon +2 more
doaj +1 more source
RB155, RB156 and RB189 antibodies do not recognize the D. discoideum Tsg101 protein by western blot
The recombinant antibodies RB155, RB156 and RB189 do not detect by western blot the full-length Tsg101 protein from Dictyostelium discoideum.
Florence Leuba, Thierry Soldati
doaj +1 more source
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
Fate and regulation of anterior-like cells in Dictyostelium slugs [PDF]
A pattern of two tissue types exists in the Dictyostelium slug. Contained within the posterior tissue are anterior-like cells which comprise about 10% of the developing cell mass.
Sternfeld, John +3 more
core +1 more source
Five recombinant antibodies ABCD_RB872, ABCD_RB873, ABCD_RB874, ABCD_RB875 and ABCD_RB876 detect by ELISA a purified peptide of Dictyostelium discoideum DstC protein.
Justine Toinon +2 more
doaj +1 more source
The AJ513 antibody recognizes the Dictyostelium p25 marker by immunofluorescence
The AJ513 antibody, derived from the H72 hybridoma, detects by immunofluorescence the p25-labelled compartments from Dictyostelium discoideum.
Wanessa C Lima, Pierre Cosson
doaj +1 more source
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley +1 more source
How phagocytic cells kill bacteria: Lessons from a professional killer
How phagocytic cells ingest and kill bacteria has been studied for more than a century, but many questions remain unanswered. The study of the amoeba Dictyostelium discoideum brings new answers, and new questions. Professional phagocytic cells such as neutrophils and macrophages, as well as free‐living soil amoebae like Dictyostelium discoideum, employ
Otmane Lamrabet, Pierre Cosson
wiley +1 more source
Developmental decisions in Dictyostelium discoideum
Dictyostelium discoideum is an excellent system in which to study developmental decisions. Synchronous development is triggered by starvation and rapidly generates a limited number of cell types. Genetic and image analyses have revealed the elegant intricacies associated with this simple development system.
Strmecki, L, Greene, D, Pears, C
openaire +3 more sources

