Results 1 to 10 of about 17,348 (180)

Development of a Positive Selection High Throughput Genetic Screen in Dictyostelium discoideum

Frontiers in Cell and Developmental Biology, 2021
The cellular slime mold Dictyostelium discoideum is a powerful model organism that can be utilized to investigate human health and disease. One particular strength of Dictyostelium is that it can be utilized for high throughput genetic screens.
Felicia N. Williams, Yumei Wu, K. Matthew Scaglione, K. Matthew Scaglione, K. Matthew Scaglione   +4 more
doaj   +1 more source

Resistance of Dictyostelium discoideum membranes to saponin permeabilization

BMC Research Notes, 2010
Background Saponin is a mild detergent commonly used to permeabilize cells prior to immunofluorescence labeling of intracellular proteins. It has previously been used to that effect in Dictyostelium discoideum amoebae.
Cosson Pierre, Mercanti Valentina
doaj   +1 more source

Multi-color fluorescence live-cell imaging in Dictyostelium discoideum

Cell Structure and Function
The cellular slime mold Dictyostelium discoideum, a member of the Amoebozoa, has been extensively studied in cell and developmental biology. D. discoideum is unique in that they are genetically tractable, with a wealth of data accumulated over half a ...
Hidenori Hashimura, Satoshi Kuwana, Hibiki Nakagawa, Kenichi Abe, Tomoko Adachi, Toyoko Sugita, Shoko Fujishiro, Gen Honda, Satoshi Sawai   +8 more
doaj   +1 more source

Protection of spores from ultraviolet-C irradiation by auto-fluorescent substances in the spore mass of the cellular slime mold Dictyostelium discoideum

Pteridines, 2014
In this study, native spores surrounded by fluorescent substances in the spore mass of Dictyostelium discoideum were found to be resistant to relatively strong ultraviolet-C (UV-C) irradiation (2880 J/m2).
Uchiyama Saburo, Hatakeyama Ikuo
doaj   +1 more source

Quantitative analysis by flow cytometry of green fluorescent protein-tagged human phenylalanine hydroxylase expressed in Dictyostelium

Pteridines, 2017
We have developed a fluorescence assay system to monitor the protein levels of human phenylalanine hydroxylase (hPAH). Wild-type (WT) and three mutant hPAHs (I65T, L255V, and S349L) were expressed as green fluorescent protein (GFP)-tagged forms in a PAH ...
Kim Hye-Lim, Ryu Hyun-Chul, Park Young Shik   +2 more
doaj   +1 more source

The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum

BioTechniques, 2013
The loading controls used for quantitative immunoblotting of mammalian proteins are not appropriate for use with Dictyostelium discoideum. Actin levels, for example, change greatly during Dictyostelium development.
Andrew J. Davidson, Jason S. King, Robert H. Insall   +2 more
doaj   +1 more source

Identification and recombinant expression of anandamide hydrolyzing enzyme from Dictyostelium discoideum

BMC Microbiology, 2012
Background Anandamide (Arachidonoyl ethanolamide) is a potent bioactive lipid studied extensively in humans, which regulates several neurobehavioral processes including pain, feeding and memory.
Neelamegan Dhamodharan, Schoenhofen Ian C, Richards James C, Cox Andrew D   +3 more
doaj   +1 more source

Acute stress and multicellular development alter the solubility of the Dictyostelium Sup35 ortholog ERF3

Microbiology Spectrum
Among sequenced organisms, the genome of Dictyostelium discoideum is unique in that it encodes for a massive amount of repeat-rich sequences in the coding region of genes.
Felicia N. Williams, Kanesha L. Travis, Holly N. Haver, Anna D. Umano, Yaneli Guerra-Hernandez, K. Matthew Scaglione   +5 more
doaj   +1 more source

Insights on Microsatellite Characteristics, Evolution, and Function From the Social Amoeba Dictyostelium discoideum

Frontiers in Neuroscience, 2022
Microsatellites are repetitive sequences commonly found in the genomes of higher organisms. These repetitive sequences are prone to expansion or contraction, and when microsatellite expansion occurs in the regulatory or coding regions of genes this can ...
Felicia N. Williams, K. Matthew Scaglione, K. Matthew Scaglione, K. Matthew Scaglione   +3 more
doaj   +1 more source

Iron metabolism and resistance to infection by invasive bacteria in the social amoeba Dictyostelium discoideum

Frontiers in Cellular and Infection Microbiology, 2013
Dictyostelium cells are forest soil amoebae, which feed on bacteria and proliferate as solitary cells until bacteria are consumed. Starvation triggers a change in life style, forcing cells to gather into aggregates to form multicellular organisms ...
Salvatore eBozzaro, Simona eBuracco, Barbara ePeracino   +2 more
doaj   +1 more source