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Multi-color fluorescence live-cell imaging in Dictyostelium discoideum [PDF]

Cell Structure and Function
The cellular slime mold Dictyostelium discoideum, a member of the Amoebozoa, has been extensively studied in cell and developmental biology. D. discoideum is unique in that they are genetically tractable, with a wealth of data accumulated over half a ...
Hidenori Hashimura, Satoshi Kuwana, Hibiki Nakagawa, Kenichi Abe, Tomoko Adachi, Toyoko Sugita, Shoko Fujishiro, Gen Honda, Satoshi Sawai   +8 more
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Dictyostelium discoideum protocols

European Journal of Histochemistry, 2013
As well highlighted and stressed by the two editors (Ludwig Eichinger, Center for Biochemistry, Medical Faculty, University of Cologne, Köln, Germany and Francisco Rivero, Center for Cardiovascular and Metabolic research, the Hull York Medical School ...
Carlo Alberto Redi
doaj   +2 more sources

A recombinant antibody toolbox for Dictyostelium discoideum

BMC Research Notes, 2020
Objective The amoeba Dictyostelium discoideum has been a valuable model organism to study numerous facets of eukaryotic cell biology, such as cell motility, cell adhesion, macropinocytosis and phagocytosis, host–pathogen interactions and multicellular ...
Wanessa C. Lima, Philippe Hammel, Pierre Cosson   +2 more
doaj   +1 more source

Insights on Microsatellite Characteristics, Evolution, and Function From the Social Amoeba Dictyostelium discoideum

Frontiers in Neuroscience, 2022
Microsatellites are repetitive sequences commonly found in the genomes of higher organisms. These repetitive sequences are prone to expansion or contraction, and when microsatellite expansion occurs in the regulatory or coding regions of genes this can ...
Felicia N. Williams, K. Matthew Scaglione, K. Matthew Scaglione, K. Matthew Scaglione   +3 more
doaj   +1 more source

The RB008 antibody does not recognize D. discoideum NoxA by western blot

Antibody Reports, 2020
The recombinant antibody RB008 does not detect by western blot the Dictyostelium discoideum protein NoxA expressed in D. discoideum and in two human cell-lines.
Florence Leuba, Thierry Soldati
doaj   +1 more source

The AN703 antibody recognizes Dictyostelium discoideum PDI by immunofluorescence

Antibody Reports, 2021
The AN703 antibody, derived from the 221-42-1 hybridoma, detects PDI-containing endoplasmic reticulum of Dictyostelium discoideum by immunofluorescence.
Anna Marchetti
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A Heat Shock Protein 48 (HSP48) Biomolecular Condensate Is Induced during Dictyostelium discoideum Development

mSphere, 2019
The social amoeba Dictyostelium discoideum’s proteome contains a vast array of simple sequence repeats, providing a unique model to investigate proteostasis. Upon conditions of cellular stress, D.
Stephanie Santarriaga, Alicia Fikejs, Jamie Scaglione, K. Matthew Scaglione   +3 more
doaj   +1 more source

Flow-driven instabilities during pattern formation of Dictyostelium discoideum

New Journal of Physics, 2015
The slime mold Dictyostelium discoideum is a well known model system for the study of biological pattern formation. In the natural environment, aggregating populations of starving Dictyostelium discoideum cells may experience fluid flows that can ...
A Gholami, O Steinbock, V Zykov, E Bodenschatz   +3 more
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Dictyostelium discoideum as a novel host system to study the interaction between phagocytes and yeasts

Frontiers in Microbiology, 2016
The social amoeba Dictyostelium discoideum is a well-established model organism to study the interaction between bacteria and phagocytes. In contrast, research using D. discoideum as a host model for fungi is rare.
Barbara Koller, Christin Schramm, Christin Schramm, Susann Siebert, János Triebel, Eric Deland, Anna Maria Pfefferkorn, Volker Rickerts, Sascha Thewes   +8 more
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Low Base-Substitution Mutation Rate but High Rate of Slippage Mutations in the Sequence Repeat-Rich Genome of Dictyostelium discoideum

G3: Genes, Genomes, Genetics, 2020
We describe the rate and spectrum of spontaneous mutations for the social amoeba Dictyostelium discoideum, a key model organism in molecular, cellular, evolutionary and developmental biology. Whole-genome sequencing of 37 mutation accumulation lines of D.
Sibel Kucukyildirim, Megan Behringer, Way Sung, Debra A. Brock, Thomas G. Doak, Hatice Mergen, David C. Queller, Joan E. Strassmann, Michael Lynch   +8 more
doaj   +1 more source