Results 41 to 50 of about 23,071 (215)

The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum

open access: yesBioTechniques, 2013
The loading controls used for quantitative immunoblotting of mammalian proteins are not appropriate for use with Dictyostelium discoideum. Actin levels, for example, change greatly during Dictyostelium development.
Andrew J. Davidson   +2 more
doaj   +1 more source

Nonconstant Positive Steady States for a Predator–Prey System With Signal‐Dependent Diffusion

open access: yesMathematische Nachrichten, EarlyView.
ABSTRACT This paper investigates a highly nonlinear predator–prey reaction–diffusion system featuring signal‐dependent prey diffusion, where the motility of the prey is modulated by a chemical signal released by predators. For unbounded functional responses, we establish the global‐in‐time existence and uniform‐in‐time boundedness of classical ...
Qigang Deng   +3 more
wiley   +1 more source

A gradient method for the quantitative analysis of cell movement and tissue flow and its application to the analysis of multicellular Dictyostelium development [PDF]

open access: yes, 1994
We describe the application of a novel image processing method, which allows quantitative analysis of cell and tissue movement in a series of digitized video images. The result is a vector velocity field showing average direction and velocity of movement
Nomura, Atsushi   +3 more
core   +1 more source

Antibodies ABCD_RB869-RB871 do not recognize the full-length DstB by western blot

open access: yesAntibody Reports
Recombinant antibodies ABCD_RB869, ABCD_RB870 and ABCD_RB871 detect by ELISA a peptide from the Dictyostelium discoideum DstB protein. These antibodies do not recognize the endogenous DstB and a GFP-DstB protein stably expressed in D. discoideum cells by
Justine Toinon, Thierry Soldati
doaj   +1 more source

The lipid composition and membrane fluidity of Dictyostelium discoideum plasma membranes at various stages during differentiation.

open access: yesJournal of Lipid Research, 1980
There are only minor changes in the amounts of the major lipid constituents of Dictyostelium discoideum plasma membranes during the early stages in the differentiation of this organism.
G Weeks, F G Herring
doaj   +1 more source

Beyond single cells: Ten years of commercial lattice light‐sheet microscopy

open access: yesJournal of Microscopy, EarlyView.
Abstract Lattice light‐sheet microscopy (LLSM) enables gentle, rapid, volumetric fluorescence imaging of living biological systems. The technique employs Bessel beam interference to produce a light‐sheet which retains its thickness over a relatively large field‐of‐view compared to other light‐sheets.
Helena L. E. Coker, David S. Corcoran
wiley   +1 more source

Alkaline Phosphatase in Dictyostelium discoideum [PDF]

open access: yesJournal of General Microbiology, 1965
Several phosphatases have been demonstrated in Dictyostelium discoideum. The alkaline phosphatase, pH optimum 9·0, is a 5′-nucleotidase, attacking adenosine monophosphate and deoxyadenosine monophosphate, but catalyses also hydrolysis of p-nitrophenyl phosphate.
K, GEZELIUS, B E, WRIGHT
openaire   +2 more sources

The Social Amoeba Polysphondylium pallidum Loses Encystation and Sporulation, but Can Still Erect Fruiting Bodies in the Absence of Cellulose [PDF]

open access: yes, 2014
Amoebas and other freely moving protists differentiate into walled cysts when exposed to stress. As cysts, amoeba pathogens are resistant to biocides, preventing treatment and eradication.
Du, Qingyou, Schaap, Pauline; id_orcid
core   +1 more source

ABCD_RC012 recognizes Dictyostelium discoideum AplA protein by ELISA and western blot

open access: yesAntibody Reports
Four recombinant antibodies ABCD_RC012 to ABCD_RC015 were shown to detect the purified Dictyostelium discoideum AplA protein by ELISA. In addition, the ABCD_RC012 antibody recognizes AplA by western blot in non-reducing conditions in D. discoideum cells
Anna Marchetti, Philippe Hammel
doaj   +1 more source

RB014, RB015 and RB016 antibodies recognize a peptide of the D. discoideum Phg1A protein by western blot

open access: yesAntibody Reports, 2020
Recombinant antibodies RB014, RB015 and RB016 detect by western blot a peptide of the Dictyostelium discoideum Phg1a protein fused to a GST protein.
Madeleine Zufferey, Cedric Blanc
doaj   +1 more source

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