Results 21 to 30 of about 13,118 (184)

The AK423 antibody recognizes Dictyostelium actin network by immunofluorescence

Antibody Reports, 2019
The AK423 antibody, derived from the 224-236-1 hybridoma, detects by immunofluorescence the actin network of Dictyostelium discoideum.
Wanessa C Lima
doaj   +1 more source

The lipid composition and membrane fluidity of Dictyostelium discoideum plasma membranes at various stages during differentiation.

Journal of Lipid Research, 1980
There are only minor changes in the amounts of the major lipid constituents of Dictyostelium discoideum plasma membranes during the early stages in the differentiation of this organism.
G Weeks, F G Herring
doaj   +1 more source

RA043 and RA044 antibodies recognize a peptide of the D. discoideum SibC protein by western blot

Antibody Reports, 2020
Recombinant antibodies RA043 and RA044 detect by western blot a peptide of the Dictyostelium discoideum SibC protein fused to a GST protein.
Madeleine Zufferey, Cedric Blanc
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Antibodies ABCD_RB869-RB871 do not recognize the full-length DstB by western blot

Antibody Reports
Recombinant antibodies ABCD_RB869, ABCD_RB870 and ABCD_RB871 detect by ELISA a peptide from the Dictyostelium discoideum DstB protein. These antibodies do not recognize the endogenous DstB and a GFP-DstB protein stably expressed in D. discoideum cells by
Justine Toinon, Thierry Soldati
doaj   +1 more source

The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum

BioTechniques, 2013
The loading controls used for quantitative immunoblotting of mammalian proteins are not appropriate for use with Dictyostelium discoideum. Actin levels, for example, change greatly during Dictyostelium development.
Andrew J. Davidson, Jason S. King, Robert H. Insall   +2 more
doaj   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

ABCD_RC012 recognizes Dictyostelium discoideum AplA protein by ELISA and western blot

Antibody Reports
Four recombinant antibodies ABCD_RC012 to ABCD_RC015 were shown to detect the purified Dictyostelium discoideum AplA protein by ELISA. In addition, the ABCD_RC012 antibody recognizes AplA by western blot in non-reducing conditions in D. discoideum cells
Anna Marchetti, Philippe Hammel
doaj   +1 more source

AF291 and AE391 antibodies recognize a Dictyostelium HA-tagged protein by Western blot

Antibody Reports, 2019
The AF291 and AE391 antibodies detect an HA-tagged Dictyostelium discoideum protein by Western blot.
Tania Jauslin
doaj   +1 more source

RB740, RB741 and RB742 antibodies recognize the Dicytostelium discoideum Talin B protein by ELISA

Antibody Reports, 2022
The recombinant antibodies RB740, RB741 and RB742 detect by ELISA the Dictyostelium discoideum Talin-B fused to a GST protein.
Philippe Hammel, Srishti Rangarajan
doaj   +1 more source