Results 41 to 50 of about 28,273 (219)

Resistance of Dictyostelium discoideum membranes to saponin permeabilization

BMC Research Notes, 2010
Background Saponin is a mild detergent commonly used to permeabilize cells prior to immunofluorescence labeling of intracellular proteins. It has previously been used to that effect in Dictyostelium discoideum amoebae.
Cosson Pierre, Mercanti Valentina
doaj   +1 more source

Investigating the Effect of Emetic Compounds on Chemotaxis in Dictyostelium Identifies a Non-Sentient Model for Bitter and Hot Tastant Research. [PDF]

, 2011
Novel chemical entities (NCEs) may be investigated for emetic liability in a range of unpleasant experiments involving retching, vomiting or conditioned taste aversion/food avoidance in sentient animals.
Paul L. R. Andrews, Steven Robery, Robin S B Williams, Robin S. B. Williams, Robery, Steven, Percie du Sert Nathalie, Andrews, PL, Percie du Sert, N, Williams, RS, Andrews, Paul L. R., Williams, Robin S. B., Williams Robin S. B., Robery, S, Andrews Paul L. R., Percie du Sert, Nathalie, Mukanowa, J, Paul L R Andrews, Mukanowa Janina, Robery Steven, Mukanowa, Janina, Janina Mukanowa, Nathalie Percie du Sert   +21 more
core   +1 more source

Comparative genomics of the social amoebae Dictyostelium discoideum and Dictyostelium purpureum [PDF]

Genome Biology, 2011
Abstract Background The social amoebae (Dictyostelia) are a diverse group of Amoebozoa that achieve multicellularity by aggregation and undergo morphogenesis into fruiting bodies with terminally differentiated spores and stalk cells.
Sucgang, Richard, Kuo, Alan, Tian, Xiangjun, Salerno, William, Parikh, Anup, Feasley, Christa L, Dalin, Eileen, Tu, Hank, Huang, Eryong, Barry, Kerrie, Lindquist, Erika, Shapiro, Harris, Bruce, David, Schmutz, Jeremy, Salamov, Asaf, Fey, Petra, Gaudet, Pascale, Anjard, Christophe, Madan Babu, M, Basu, Siddhartha, Bushmanova, Yulia, van der Wel, Hanke, Katoh-Kurasawa, Mariko, Dinh, Christopher, Coutinho, Pedro M, Saito, Tamao, Elias, Marek, Schaap, Pauline, Kay, Robert R, Henrissat, Bernard, Eichinger, Ludwig, Rivero, Francisco, Putnam, Nicholas H, West, Christopher M, Loomis, William F, Chisholm, Rex L, Shaulsky, Gad, Strassmann, Joan E, Queller, David C, Kuspa, Adam, Grigoriev, Igor V   +40 more
openaire   +3 more sources

Multi-color fluorescence live-cell imaging in Dictyostelium discoideum

Cell Structure and Function
The cellular slime mold Dictyostelium discoideum, a member of the Amoebozoa, has been extensively studied in cell and developmental biology. D. discoideum is unique in that they are genetically tractable, with a wealth of data accumulated over half a ...
Hidenori Hashimura, Satoshi Kuwana, Hibiki Nakagawa, Kenichi Abe, Tomoko Adachi, Toyoko Sugita, Shoko Fujishiro, Gen Honda, Satoshi Sawai   +8 more
doaj   +1 more source

Quantitative analysis by flow cytometry of green fluorescent protein-tagged human phenylalanine hydroxylase expressed in Dictyostelium

Pteridines, 2017
We have developed a fluorescence assay system to monitor the protein levels of human phenylalanine hydroxylase (hPAH). Wild-type (WT) and three mutant hPAHs (I65T, L255V, and S349L) were expressed as green fluorescent protein (GFP)-tagged forms in a PAH ...
Kim Hye-Lim, Ryu Hyun-Chul, Park Young Shik   +2 more
doaj   +1 more source

Quantitative Analysis of Cell Motility and Chemotaxis in Dictyostelium discoideum by Using an Image Processing System and a Novel Chemotaxis Chamber Providing Stationary Chemical Gradients [PDF]

, 1989
An image processing system was programmed to automatically track and digitize the movement of amebae under phase-contrast microscopy. The amebae moved in a novel chemotaxis chamber designed to provide stable linear attractant gradients in a thin agarose ...
G Gerisch, Fisher, P. R., Merkl, Rainer, Gerisch, G., P R Fisher, R Merkl   +5 more
core   +1 more source

Triparental inheritance in Dictyostelium [PDF]

Proceedings of the National Academy of Sciences, 2018
Sex promotes the recombination and reassortment of genetic material and is prevalent across eukaryotes, although our knowledge of the molecular details of sexual inheritance is scant in several major lineages. In social amoebae, sex involves a promiscuous mixing of cytoplasm before zygotes consume the majority of cells, but for ...
Gareth Bloomfield, Peggy Paschke, Marina Okamoto, Tim J. Stevens, Hideko Urushihara   +4 more
openaire   +2 more sources

The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum

BioTechniques, 2013
The loading controls used for quantitative immunoblotting of mammalian proteins are not appropriate for use with Dictyostelium discoideum. Actin levels, for example, change greatly during Dictyostelium development.
Andrew J. Davidson, Jason S. King, Robert H. Insall   +2 more
doaj   +1 more source

RNAi in the Rhizarian Phytopathogen Plasmodiophora brassicae: The Causal Agent of Clubroot Disease in Cruciferous Crops

Advanced Science, EarlyView.
This study uncovers an unusual RNAi pathway in the rhizarian pathogen Plasmodiophora brassicae. In the absence of Dicer, a Drosha‐like RNase III protein supports the biogenesis of predominant 21‐nt small RNAs, and two Argonaute proteins mediate small RNA‐guided silencing.
Xiong Zhang, Yupo Wu, Jiachang Hou, Lixia Gao, Longqi Pan, Cuicui Shen, Chuanji Zhao, Feng Gao, Yi Zhang, Li Ren, Xiaoping Fang, Xiaoling Dun, Shengyi Liu, Lijiang Liu   +13 more
wiley   +1 more source

Analysis of optical density wave propagation and cell movement in the cellular slime mould Dictyostelium discoideum [PDF]

, 1991
We have studied optical density wave propagation during aggregation of the cellular slime mould Dictyostelium discoideum in a quantitative manner by digital image analysis.
Siegert, Florian, Weijer, Cornelis J.
core   +1 more source