Results 41 to 50 of about 42,471 (213)

The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum

BioTechniques, 2013
The loading controls used for quantitative immunoblotting of mammalian proteins are not appropriate for use with Dictyostelium discoideum. Actin levels, for example, change greatly during Dictyostelium development.
Andrew J. Davidson, Jason S. King, Robert H. Insall   +2 more
doaj   +1 more source

SILAC-based proteomic quantification of chemoattractant-induced cytoskeleton dynamics on a second to minute timescale [PDF]

, 2014
Cytoskeletal dynamics during cell behaviours ranging from endocytosis and exocytosis to cell division and movement is controlled by a complex network of signalling pathways, the full details of which are as yet unresolved.
A Bagorda, A Kortholt, A Kortholt, A Para, A Shevchenko, AJ Saldanha, AT Sasaki, C Orelio, CL Chen, CL Manahan, DM Veltman, DM Veltman, DM Veltman, EC Rericha, EL de Hostos, F Friedberg, F Vazquez, G Vlahou, H Cai, H Kae, HR Bourne, I Marin, J Condeelis, J Cox, J Faix, J Riedl, J Schindelin, J Yan, JF Cote, JF Cote, JW Han, JY Kim, KF Swaney, L Bosgraaf, L Bosgraaf, L Bosgraaf, L Chen, M Affolter, M Brenner, M de la Roche, M Patel, MJ de Hoon, MK Vartiainen, MR Lee, MS Boguski, N Ibarra, N Meller, OD Weiner, R Insall, RH Insall, RJ Eddy, S Hanna, S Levi, SE Ong, SJ Allen, SL Blagg, TJ Jeon, WN van Egmond, X Xu, Y Yang, YC Wu   +60 more
core   +3 more sources

Quantitative analysis by flow cytometry of green fluorescent protein-tagged human phenylalanine hydroxylase expressed in Dictyostelium

Pteridines, 2017
We have developed a fluorescence assay system to monitor the protein levels of human phenylalanine hydroxylase (hPAH). Wild-type (WT) and three mutant hPAHs (I65T, L255V, and S349L) were expressed as green fluorescent protein (GFP)-tagged forms in a PAH ...
Kim Hye-Lim, Ryu Hyun-Chul, Park Young Shik   +2 more
doaj   +1 more source

Identification and recombinant expression of anandamide hydrolyzing enzyme from Dictyostelium discoideum

BMC Microbiology, 2012
Background Anandamide (Arachidonoyl ethanolamide) is a potent bioactive lipid studied extensively in humans, which regulates several neurobehavioral processes including pain, feeding and memory.
Neelamegan Dhamodharan, Schoenhofen Ian C, Richards James C, Cox Andrew D   +3 more
doaj   +1 more source

Self-organized Vortex State in Two-dimensional Dictyostelium Dynamics

, 1999
We present results of experiments on the dynamics of Dictyostelium discoideum in a novel set-up which constraints cell motion to a plane. After aggregation, the amoebae collect into round ''pancake" structures in which the cells rotate around the center ...
Alastair Nicol, Armand Sarkissian, B. L. Partridge, B. N. Vasiev, B. Vasiev, B. Wang, D. Helbing, E. Albano, E. Ben-Jacob, E. Ben-Jacob, E. M. Rauch, E. Palsson, F. Alacantra, F. Siegert, H. J. Bussemaker, H. Levine, H. Levine, Herbert Levine, J. A. Glazier, J. C. Dallon, J. Hemmingson, J. Lauzeral, J. Rietdorf, J. T. Bonner, J. Toner, K. J. Lee, K. Nagel, M. Falcke, N. Savil, P. C. Newell, P. N. Devreotes, R. Escalante, R. N. Smith, S. Bozzaro, T. Hofer, T. Hofer, T. Hofer, T. P. Stossel, T. Vicsek, W. Loomis, W. Ward, William F. Loomis, Wouter-Jan Rappel, Y. Jiang, Y. L. Duparcmeur, Z. Xiao   +45 more
core   +1 more source

GenePath: a System for Automated Construction of Genetic Networks from Mutant Data [PDF]

, 2003
Motivation: Genetic pathways are often used in the analysis of biological phenomena. In classical genetics, they are constructed manually from experimental data on mutants. The field lacks formalism to guide such analysis, and accounting for all the data
Bratko, Ivan, Demšar, Janez, Halter, John, Juvan, Peter, Kuspa, Adam, Shaulsky, Gad, Zupan, Blaz   +6 more
core   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Information Transmission Strategies for Self‐Organized Robotic Aggregation

Advanced Robotics Research, EarlyView.
In this review, we discuss how information transmission influences the neighbor‐based self‐organized aggregation of swarm robots. We focus specifically on local interactions regarding information transfer and categorize previous studies based on the functions of the information exchanged.
Shu Leng, Zihan Li, Yifeng Shao, Jinhao Liu, Li Yu, Shuguang Li   +5 more
wiley   +1 more source

Bacterial Survival in Dictyostelium [PDF]

BIO-PROTOCOL, 2017
We performed an assay to test the ability of different E. coli strains to survive inside amoebal cells after ingestion. In the assay we incubated bacteria together with cells of Dictyostelium discoideum for six hours. After co-incubation most of the uningested bacteria were removed by centrifugation and the remaining uningested bacteria were killed by ...
Regin Rønn, Xiuli Hao, Freja Lüthje, Nadezhda German, Xuanji Li, Fuyi Huang, Javan Kisaka, David Huffman, Hend Alwathnani, Yong-Guan Zhu, Christopher Rensing   +10 more
openaire   +3 more sources

Insights on Microsatellite Characteristics, Evolution, and Function From the Social Amoeba Dictyostelium discoideum

Frontiers in Neuroscience, 2022
Microsatellites are repetitive sequences commonly found in the genomes of higher organisms. These repetitive sequences are prone to expansion or contraction, and when microsatellite expansion occurs in the regulatory or coding regions of genes this can ...
Felicia N. Williams, K. Matthew Scaglione, K. Matthew Scaglione, K. Matthew Scaglione   +3 more
doaj   +1 more source