Results 121 to 130 of about 213,817 (308)

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

Airborne lidar observations of water vapour variability and transport

open access: yes, 2012
Water vapour, a minor constituent of the earth’s atmosphere, plays a major role in the radiation budget and the water cycle with important implications for weather and climate. Due to the heterogeneous distribution of its sources, evaporation, and sinks,
Fischer, Lucas   +4 more
core  

THEORETICAL ANALYSIS OF DIFFERENTIAL ABSORPTION PHOTOACOUSTIC SPECTROSCOPY (DAPAS)

open access: yes, 1983
On propose une méthode nouvelle de "spectroscopie photoacoustique par absorption différentielle" (DAPAS). La théorie montre une amélioration importante du rapport signal-sur-bruit en utilisant cette méthode.A new method, "differential absorption ...
T. Hoshimiya
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

open access: yesFEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Differential absorption lidar for water vapour in the atmosphere [PDF]

open access: yes, 2002
Looks at the development of Differential Absorption Lidar based on diode lasers for profiling the temporal and vertical distribution of water vapour in the atmosphere.
Bae, Kwang-Ho
core  

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

open access: yesFEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel   +4 more
wiley   +1 more source

Accuracy Evaluation of Differential Absorption Lidar for Ozone Detection and Intercomparisons with Other Instruments

open access: yesRemote Sensing
Differential absorption lidar is an advanced tool for investigating tropospheric ozone transport and development. High-quality differential absorption lidar data are the basis for studying the temporal and spatial evolution of ozone pollution.
Guangqiang Fan   +8 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

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