Results 241 to 250 of about 2,635,483 (291)
Some of the next articles are maybe not open access.
Molecular Biotechnology, 1998
Differential display of mRNA (DD) is a technique in which mRNA species expressed by a cell population are reverse transcribed and then amplified by many separate polymerase chain reactions (PCR). PCR primers and conditions are chosen so that any given reaction yields a limited number of amplified cDNA fragments, permitting their visualization as ...
J S, Zhang +3 more
openaire +2 more sources
Differential display of mRNA (DD) is a technique in which mRNA species expressed by a cell population are reverse transcribed and then amplified by many separate polymerase chain reactions (PCR). PCR primers and conditions are chosen so that any given reaction yields a limited number of amplified cDNA fragments, permitting their visualization as ...
J S, Zhang +3 more
openaire +2 more sources
Differential display in the time of microarrays
Expert Review of Molecular Diagnostics, 2002A brief overview of major methods used for genome-wide expression profiling is presented. Special attention is devoted to ordered differential display, subtractive hybridization and DNA microarrays. Future prospects of comparative gene expression studies using combinations of differential display methods and microarray technology are outlined.
Natalia E Broude
exaly +3 more sources
2005
Ordered differential display (ODD) is one of the approaches that uses systematic, rather than random, sampling of transcripts for display and thereby provides means to browse through essentially all the transcripts in the compared mRNA pools. It is specifically adapted for small amounts of starting material.
Mikhail V, Matz, Ella A, Meleshkevitch
openaire +2 more sources
Ordered differential display (ODD) is one of the approaches that uses systematic, rather than random, sampling of transcripts for display and thereby provides means to browse through essentially all the transcripts in the compared mRNA pools. It is specifically adapted for small amounts of starting material.
Mikhail V, Matz, Ella A, Meleshkevitch
openaire +2 more sources
Molecular Biology Reports, 2006
Differential display (DD) is a novel PCR-based technique, very commonly used to study differentially expressed genes at the mRNA level. In this paper we report a modified version of this technique that we have used to study the differences between the mRNA population from brain tissue of adult and old rats.
Rashmi U, Pathak, M S, Kanungo
openaire +2 more sources
Differential display (DD) is a novel PCR-based technique, very commonly used to study differentially expressed genes at the mRNA level. In this paper we report a modified version of this technique that we have used to study the differences between the mRNA population from brain tissue of adult and old rats.
Rashmi U, Pathak, M S, Kanungo
openaire +2 more sources
Trouble with differential display
Trends in Biotechnology, 2002Differential Display. Edited by Ronald A. Leslie and Harold A. Robertson. Oxford University Press, 2000. US$ 115.00 (hbk) (274 pages) ISBN 0 19 9637598 XDifferential display (DD) is a well-developed method for comparative cDNA expression studies. Its advantages and limitations have been widely discussed both in periodic publications and books.
Natalia E Broude
exaly +2 more sources
Recent advances in differential display
Current Opinion in Immunology, 1995Differential display and RNA arbitrary primed polmerase chain reaction are methods recently designed to identify and isolate differentially expressed genes. Methodological modifications have since been introduced to streamline the techniques. The major effort has centered on how to eliminate false positives as approached from a variety of angles ...
P, Liang, A B, Pardee
openaire +2 more sources
Using differential display in the study of adipocyte differentiation
Biochemical Society Transactions, 1994Study of the programme of adipose conversion displayed by mouse 3T3L1 adipocyte precursors and control 3T3C2 cells [l-31 using the recently developed differential display [4-61 technique have been initiated. The two 3T3 fibroblast cell sub-lines differ in their ability to develop the biochemical characteristics of mature adipocytes and the process can ...
W D, Edwards, H L, Jessop, A, Cryer
openaire +2 more sources
2000
Abstract When the technique of differential display was first described in the early 1990s, sequence information existed for a few thousand human genes and some functional information was available for most of those. At about that time, the development of high throughput sequencing technologies and large genome databases, including ...
openaire +1 more source
Abstract When the technique of differential display was first described in the early 1990s, sequence information existed for a few thousand human genes and some functional information was available for most of those. At about that time, the development of high throughput sequencing technologies and large genome databases, including ...
openaire +1 more source
Differential display and protein quantification
2007High-throughput quantitation of proteins is of essential importance for all systems biology approaches and provides complementary information on steady-state gene expression and perturbation-induced systems responses. This information is necessary because it is, e.g., difficult to predict protein concentrations from the level of mRNAs, since regulatory
Erich, Brunner +3 more
openaire +2 more sources
1997
Most biological processes rely on changes in the level and pattern of gene expression. Their molecular and functional dissection often requires the isolation and subsequent characterization of genes that are differentially expressed in space (different tissues or organs), in time (different developmental stages), or in only one sex.
George Dimopoulos, Christos Louis
openaire +2 more sources
Most biological processes rely on changes in the level and pattern of gene expression. Their molecular and functional dissection often requires the isolation and subsequent characterization of genes that are differentially expressed in space (different tissues or organs), in time (different developmental stages), or in only one sex.
George Dimopoulos, Christos Louis
openaire +2 more sources