Results 71 to 80 of about 44,664 (309)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Materiais SiO2-TiO2 para a degradação fotocatalítica de diuron

open access: yesQuímica Nova, 2011
SiO2-TiO2 materials prepared by sol-gel method were evaluated in the photocatalytic degradation of diuron. The materials were prepared with and without surfactant cetyltrimethylammonium chloride at different temperatures (25, 50 and 100 ºC).
Arthur Alaím Bernardes   +5 more
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Autofluorescence and diffuse reflectance spectroscopy for oral oncology

open access: yes, 2005
Background and Objectives: Autofluorescence and diffuse reflectance spectroscopy have been used separately and combined for tissue diagnostics. Previously, we assessed the value of autofluorescence spectroscopy for the classification of oral (pre ...
Roodenburg, JLN   +7 more
core   +2 more sources

Three-Dimensional Surface Reconstruction for Specular/Diffuse Composite Surfaces

open access: yesSensors
This paper presents an effective three-dimensional (3D) surface reconstruction technique aimed at profiling composite surfaces with both specular and diffuse reflectance.
Chung-Hsuan Huang   +4 more
doaj   +1 more source

Discovery of the Linear Region of Near Infrared Diffuse Reflectance Spectra Using the Kubelka-Munk Theory

open access: yesFrontiers in Chemistry, 2018
Particle size is of great importance for the quantitative model of the NIR diffuse reflectance. In this paper, the effect of sample particle size on the measurement of harpagoside in Radix Scrophulariae powder by near infrared diffuse (NIR) reflectance ...
Shengyun Dai   +6 more
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

open access: yesFEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke   +4 more
wiley   +1 more source

Optical determination of snow density via sub-surface scattering

open access: yesCommunications Physics
The physical properties of snow, such as its stiffness, reflectivity, and thermal conductivity, are critical components in feedback processes of the Earth’s system and useful proxies for various applications in environmental science, ranging from ...
Lars Mewes   +3 more
doaj   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

open access: yesFEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young   +7 more
wiley   +1 more source

Comparison of Diffuse Reflectance and Diffuse Transmittance Vis/NIR Spectroscopy for Assessing Soluble Solids Content in Kiwifruit Coupled with Chemometrics

open access: yesApplied Sciences
Kiwifruit, as a climacteric fruit, undergoes rapid ripening and senescence after harvest, making it highly susceptible to softening, rotting, and spoilage. Therefore, monitoring the key quality parameters of kiwifruit, particularly the accurate detection
Yu Xia   +8 more
doaj   +1 more source

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