Results 81 to 90 of about 758,669 (240)

Tratamiento anaerobio de residuos de la industria alimentaria [PDF]

, 2008
Las corrientes residuales de la industria agroalimentaria presentan un elevado contenido de materia orgánica biodegradable. El tratamiento clásico ha sido la aplicación de procesos físico-químicos y biológicos aerobios, cuyas ...
Fernández García, Belén, Flotats Ripoll, Xavier   +1 more
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Caracterización del contenido de aceite de dos oleaginosas / sacha inchi (Plukenetia volubilis L.) y canola (Brassica napus y Brassica rapa). [PDF]

, 2012
The sacha inchi oil (Plukenetia volubilis L.), seed originally from Peru, recently grown in equatorial lands, possesses omega-3, omega-6, omega-9 at levels 51,94%, 34,37% and 7,97%.
Maldonado Muñoz, Santiago, Pantoja Arévalo, Lisset   +1 more
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Molecular diagnosis of Trichinella spiralis in pig sera [PDF]

, 2015
El objetivo de este proyecto fue generar una herramienta preventiva de diagnóstico, sensible y específica, basada en técnicas de avanzada en biología molecular para la triquinelosis, una de las enfermedades zoonóticas más importantes de nuestro país, con
Di Gerónimo, Vanesa, Krivokapich, Silvio Jesús, Quintana, Silvina, Recavarren, Mariana Ines, Scialfa, Exequiel Alejandro, Viera, Ivana   +5 more
core  

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

FEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu, Lanlan Li, Wenjuan Jia, Hanzhong Feng, Xiaoquan Yu, Youli Zhao, Fengzhi Yuan, Hui Ding, Junqiang Tian, Yong‐Xing He, Zhiping Wang   +10 more
wiley   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Digestión en horno de microondas para determinación de contenido de hierro y zinc totales en alimentos

Tecnología en Marcha, 2012
En el presente estudio se optimizó el procedimiento de digestión de alimentos por medio de un horno de microondas, para cuantificar el hierro y el zinc totales en diferentes matrices por espectroscopía de absorción atómica. Se analizó la cantidad óptima
Paulina Silva-Trejos
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

EFECTO DE LA ADICIÓN DE CEPAS PROBIÓTICAS SOBRE METABOLITOS SANGUÍNEOS EN CERDOS EN CRECIMIENTO

Biotecnología en el Sector Agropecuario y Agroindustrial
El destete es la fase más crítica del cerdo, produciendo trastornos intestinales y diarreas. Para prevenirlo, se han utilizado antibióticos promotores de crecimiento (APC), generando residuos medicamentosos en el producto final.
SANTIAGO LONDOÑO-PÉREZ, JAIME PARRA-SUESCÚN   +1 more
doaj   +1 more source