Results 71 to 80 of about 37,398,274 (259)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Adapting Voice Assistant Technology for Older Adults: A Comprehensive Study on Usability, Learning Patterns, and Acceptance

open access: yesDigital
This study investigates the integration, usability, and learning patterns associated with voice assistant technology among older adults, focusing on the “Amazon Echo Show 10, 3rd generation” as a case study.
Dietmar Jakob   +4 more
doaj   +1 more source

clij/clijx-assistant: 0.6.0.1

open access: yes, 2021
Stable parts of the code have been moved to https://github.com/clij/clij2-assistant See also https://github.com/clij/clij2/releases/tag/2.5.0.1, https://github.com/clij/clijx/releases/tag/0.32.0.
Stephane Rigaud   +3 more
core   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

The nexus between AI-driven capabilities and knowledge systems in digital business environments [PDF]

open access: yesKnowledge & Performance Management
Type of the article: Research article The increasing development of digital business environments has contributed to the diversification of knowledge sources globally, making smart knowledge management crucial for enhancing the accuracy of decision ...
Manal Alsammak   +3 more
doaj   +1 more source

clij/clijx-assistant-simpleitk: 0.4.1.0

open access: yes, 2020
See also https://github.com/clij/clij2/releases/tag/2.2.0.11 https://github.com/clij/clijx/releases/tag/0.30.1.0 https://github.com/clij/assistant/releases/tag/0.4.1.
Robert Haase
core   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

open access: yesFEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Alexa’s Monstrous Agency: The Horror of the Digital Voice Assistant

open access: yesLateral
First released by Amazon in 2014, the digital voice assistant Alexa allows users to connect and automate their smart home devices through the sound of their voice. Alexa’s automation of domestic spaces comes, however, with its own set of anxieties.
Nuno Galego Marques Atalaia   +1 more
doaj   +1 more source

Treatment with KCL‐286, a first‐in‐class retinoic acid receptor‐β (RARβ) agonist, ameliorates neuronal DNA damage and inflammation in a mouse model of Alzheimer's disease

open access: yesFEBS Open Bio, EarlyView.
Repair of neuronal DNA damage in Alzheimer's disease by KCL‐286. (A) Amyloid‐β oligomers and plaques impair neuronal DNA repair pathways, leading to DNA double‐strand breaks and glial activation. (B) KCL‐286 activates RARβ/RXR signalling via retinoic acid response elements (RAREs), associated with increased BRCA1 expression, enhanced DNA repair and ...
Natasha Hill   +6 more
wiley   +1 more source

Designing an AI assistant to support independent learning in first -and second-year students

open access: yesОбразование и наука
Introduction. The challenge of developing and utilising flexible learning systems has become especially pertinent with the advent of generative neural network models, which not only facilitate the creation of educational content but also transform ...
A. A. Zakharov   +3 more
doaj   +1 more source

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