Results 11 to 20 of about 574 (93)

Digital Holographic Microscopy in Veterinary Medicine—A Feasibility Study to Analyze Label-Free Leukocytes in Blood and Milk of Dairy Cows [PDF]

Animals
For several years, the determination of a differential cell count of a raw milk sample has been proposed as a more accurate tool for monitoring the udder health of dairy cows compared with using the absolute somatic cell count.
Sabine Farschtschi, Manuel Lengl, Stefan Röhrl, Christian Klenk, Oliver Hayden, Klaus Diepold, Michael W. Pfaffl   +6 more
doaj   +2 more sources

Automated Quantification and Statistical Characterization of 3D Morphological Parameters of Red Blood Cells and Blood Coagulation Structures Using Flow Cytometry with Digital Holographic Microscopy

Photonics
Label-free, high-throughput, and 3D morphological analysis of blood cells remains a major challenge in biomedical optics. In this study, we investigate this issue using flow cytometry with digital holographic microscopy (DHM) to enable real-time, label ...
Hideki Funamizu
doaj   +2 more sources

Molecularly Imprinted Polymers Exhibit Low Cytotoxic and Inflammatory Properties in Macrophages In Vitro

Applied Sciences, 2022
Molecularly imprinted polymers (MIPs) against sialic acid (SA) have been developed as a detection tool to target cancer cells. Before proceeding to in vivo studies, a better knowledge of the overall effects of MIPs on the innate immune system is needed ...
Louise Sternbæk, Martha Kimani, Kornelia Gawlitza, Knut Rurack, Birgit Janicke, Kersti Alm, Anette Gjörloff Wingren, Håkan Eriksson   +7 more
doaj   +1 more source

Digital Holographic Microscopy for Label-Free Detection of Leukocyte Alternations Associated with Perioperative Inflammation after Cardiac Surgery

Cells, 2022
In a prospective observational pilot study on patients undergoing elective cardiac surgery with cardiopulmonary bypass, we evaluated label-free quantitative phase imaging (QPI) with digital holographic microscopy (DHM) to describe perioperative ...
David Rene Steike, Michael Hessler, Eberhard Korsching, Florian Lehmann, Christina Schmidt, Christian Ertmer, Jürgen Schnekenburger, Hans Theodor Eich, Björn Kemper, Burkhard Greve   +9 more
doaj   +1 more source

Photon-starved snapshot holography

APL Photonics, 2023
Digital holography (DH) is a powerful imaging modality that is capable of capturing the object wavefront information, making it very valuable for diverse scientific research applications.
Yunping Zhang, Stanley H. Chan, Edmund Y. Lam   +2 more
doaj   +1 more source

Flipping Interferometric Module for Simultaneous Dual-Wavelength Unwrapping of Quantitative Phase Maps of Biological Cells

Frontiers in Physics, 2021
We present an imaging platform for stain-free quantitative imaging of biological cells using a simultaneous dual-wavelength holographic module. We use this module to experimentally solve the problem of 2π phase ambiguities that occurs in spatial ...
Lidor Karako, Rongli Guo, Itay Barnea, Natan T. Shaked   +3 more
doaj   +1 more source

Erythrocyte volumetric measurements in imaging flow cytometry using simultaneous three-wavelength digital holographic microscopy

Biomedical Optics Express, 2020
We report a cross-talk free simultaneous three-wavelength digital holographic microscopy setup for spectroscopic imaging of biological cells during flow. The feasibility of the proposed measurement technique is demonstrated on erythrocytes, due to their unique morphology and dependency of hemoglobin (Hb) molecule absorption on wavelength.
Turko, Nir A., Shaked, Natan T.
openaire   +2 more sources

Deep learning with digital holographic microscopy discriminates apoptosis and necroptosis

Cell Death Discovery, 2021
Regulated cell death modalities such as apoptosis and necroptosis play an important role in regulating different cellular processes. Currently, regulated cell death is identified using the golden standard techniques such as fluorescence microscopy and ...
Joost Verduijn, Louis Van der Meeren, Dmitri V. Krysko, André G. Skirtach   +3 more
doaj   +1 more source

Moving into a new dimension: Tracking migrating cells with digital holographic cytometry in 3D [PDF]

Cytometry Part A, 2018
Moving into a new dimension : Tracking migrating cells with digital holographic cytometry in ...
openaire   +2 more sources

Large field-of-view quantitative phase imaging flow cytometry using low coherence SLD based digital holographic microscopy [PDF]

AIP Advances
We present a cost-effective quantitative phase imaging flow cytometry system that utilizes a low-coherence super luminescent diode (SLD) as the illumination source for off-axis digital holographic microscopy (DHM), enabling label-free imaging of ...
Vaibhav Bansode
doaj   +1 more source