Results 61 to 70 of about 1,063 (216)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Effect of soft tissue thickness on accuracy of conventional and digital implant impression techniques

BMC Oral Health
Background Placing implants deep sub-gingivally may affect the accuracy of implant impression techniques and the fit of final restoration. Purpose The aim of this in-vitro study was to evaluate the effect of soft tissue thickness on accuracy of ...
Eman Mostafa Awad, Mohamed Maamoun ElSheikh, Azza Abd el moneim El-Segai   +2 more
doaj   +1 more source

Evaluation and Comparison of Accuracy of Complete Arch Digital and Conventional Implant Impression as Influenced by the Use of a 3D Printed Modular Splint –An In-Vitro Study

Journal of Pharmacy and Bioallied Sciences
Aim: To investigate the accuracy of Complete Arch implant impression digitally and conventionally with and without using a 3D printed modular chain splint.
Bhushan S. Pol, Ajay V. Sabane, Rajashree D. Jadhav, Pankaj P. Kadam, Arti R. Gachake, Bhagyashree G. Kalsekar   +5 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Influence of impression method and shoulder design on the marginal adaptation of CAD/CAM nanoceramic resin onlay restorations

Heliyon
Objective: This in-vitro study investigates the influence of two different impression techniques and two shoulder designs on the marginal adaptation of computer-aided design/computer-aided manufacturing restorations.
Muyue Li, Ben Ma, Zhuanyuan Zhou, Wei Liu   +3 more
doaj   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Comparison of Internal and Marginal Adaptation of Endocrowns at Different Depths Fabricated by the Digital and Conventional Impression Techniques

International Journal of Dentistry
Conclusion: Unlike the conventional impression technique, the M and I adaptation decreased by an increase in cavity depth in the digital technique; nonetheless, the digital impression technique still showed higher M and I adaptation than the conventional
Fatemeh Razavi Ardekani, Hamid Neshandar Asli, Naghme Musapoor, Mehran Falahchai   +3 more
doaj   +1 more source

Treatment with KCL‐286, a first‐in‐class retinoic acid receptor‐β (RARβ) agonist, ameliorates neuronal DNA damage and inflammation in a mouse model of Alzheimer's disease

FEBS Open Bio, EarlyView.
Repair of neuronal DNA damage in Alzheimer's disease by KCL‐286. (A) Amyloid‐β oligomers and plaques impair neuronal DNA repair pathways, leading to DNA double‐strand breaks and glial activation. (B) KCL‐286 activates RARβ/RXR signalling via retinoic acid response elements (RAREs), associated with increased BRCA1 expression, enhanced DNA repair and ...
Natasha Hill, Hassan Yousef Othman AlMuallim, Elouisa Maddock, Carl Hobbs, Earl Clarke, Maria B. Goncalves, Jonathan P. T. Corcoran   +6 more
wiley   +1 more source

Effect of terahertz irradiation on DNA damage repair in living cells

FEBS Open Bio, EarlyView.
We investigated the effect of terahertz (THz) wave irradiation on DNA double‐strand break (DSB) repair in living cells. We found that THz irradiation enhanced DSB repair at specific frequencies, whereas heat treatment inhibited it, indicating that this effect is nonthermal and frequency‐specific.
Yuya Ueno, Shota Yamazaki, Hiromichi Hoshina, Masahiko Harata   +3 more
wiley   +1 more source

A novel in vivo method to evaluate trueness of digital impressions

BMC Oral Health, 2018
Background Intraoral scanners are devices for capturing digital impressions in dentistry. Until now, several in vitro studies have assessed the trueness of digital impressions, but in vivo studies are missing.
Emad A. Albdour, Eman Shaheen, Myrthel Vranckx, Francesco Guido Mangano, Constantinus Politis, Reinhilde Jacobs   +5 more
doaj   +1 more source