Results 101 to 110 of about 1,739,210 (295)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

The Effect of Hygroscopic Wood Structure on Some Properties of Heat Treated Products Formed with the Thermowood Method

BioResources
Changes were determined for the values ​​of physical and mechanical properties resulting from hygroscopicity in some wood species after heat treatment (HT), and to reveal statistically the relationships between the properties.
Ayhan Aytin
doaj  

Nonlinear stability of the ensemble Kalman filter with adaptive covariance inflation [PDF]

, 2015
The Ensemble Kalman filter and Ensemble square root filters are data assimilation methods used to combine high dimensional nonlinear models with observed data.
Kelly, David, Majda, Andrew J, Tong, Xin T   +2 more
core  

Scalar perturbations of higher dimensional rotating and ultra-spinning black holes

, 2004
We investigate the stability of higher dimensional rotating black holes against scalar perturbations. In particular, we make a thorough numerical and analytical analysis of six-dimensional black holes, not only in the low rotation regime but in the high ...
E. Witten, E. W. Leaver, F. R. Tangherlini, George Siopsis, H. Furuhashi, H. Kodama, H. Kodama, H. Kodama, J. M. Maldacena, K. D. Kokkotas, R. Emparan, Shijun Yoshida, Vitor Cardoso, W. Gautschi, W. H. Press, W. H. Press   +15 more
core   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Dimensional Stability Properties of Medium-Density Fiberboards Produced Using Silicone-Based Chemicals

Drvna Industrija
The purpose of this study was to investigate the effects of two different silicon-based water-repellent chemicals (Dow Corning 87 and Xiameter PMX-200 1000cs) added to melamine urea formaldehyde glue (1.07 mol, 5 % melamine additive) in different ...
Meryem Ondaral, Mustafa Usta, Sedat Ondaral   +2 more
doaj   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Effect of Date Palm Cultivar, Particle Size, Panel Density and Hot Water Extraction on Particleboards Manufactured from Date Palm Fronds

Agriculture, 2015
The objective of this work was to evaluate some of the important physical and mechanical properties of particleboard panels manufactured from three different cultivars of date palm (Phoenix dactylifera) fronds, namely Saqui, Barhi and Sukkari ...
Said S. Hegazy, Khaled Ahmed
doaj   +1 more source