Results 51 to 60 of about 125,762 (338)

On the Stereochemistry of 2-Hydroxyethylphosphonate Dioxygenase

Journal of the American Chemical Society, 2011
Stereochemical investigations have shown that the conversion of 2-hydroxyethylphosphonate to hydroxymethylphosphonate by the enzyme HEPD involves removal of the pro-S hydrogen at C2 and, surprisingly, the loss of stereochemical information at C1. As a result, the mechanisms previously proposed for HEPD must be re-evaluated.
Spencer C. Peck, Robert M. Cicchillo, Friedrich Hammerschmidt, Petra Malova, Wilfred A. van der Donk, John T. Whitteck   +5 more
openaire   +3 more sources

An optimized protocol to detect high‐throughput DNA methylation from custom targeted sequences on 96 samples simultaneously

FEBS Open Bio, EarlyView.
Workflow of a high‐throughput technology for epigenotyping of differentially methylated CpGs in specific regions of the genome. The protocol works with small amounts of DNA extracted from blood or semen. The protocol consists of both enzymatic conversion of unmethylated cytosines and capture by hybridization with a custom panel.
Nathalie Iannuccelli, Sophie Valière, Julien Sarry, Cécile Donnadieu, Julie Demars   +4 more
wiley   +1 more source

Degradation of Chloroaromatics: Purification and Characterization of a Novel Type of Chlorocatechol 2,3-Dioxygenase of Pseudomonas putida GJ31 [PDF]

, 1998
A purification procedure for a new kind of extradiol dioxygenase, termed chlorocatechol 2,3-dioxygenase, that converts 3-chlorocatechol productively was developed.
Janssen, Dick B.,, Kasberg, Thomas,, Kaschabek, Stefan R.,, Mars, Astrid E.,, Müller, Dagmar,, Reineke, Walter,   +5 more
core   +2 more sources

Tryptophan metabolite atlas uncovers organ, age, and sex‐specific variations

FEBS Open Bio, EarlyView.
Tryptophan metabolites were analyzed across twelve organs, the central nervous system, and serum in male and female mice at three life stages. We found tissue‐, sex‐, and age‐specific differences, including increased indole‐3‐pyruvate and kynurenine in aging males.
Lizbeth Perez‐Castro, Afshan F. Nawas, Jessica A. Kilgore, Pedro A. S. Nogueira, M.Carmen Lafita‐Navarro, Paul H. Acosta, Roy Garcia, Noelle S. Williams, Maralice Conacci‐Sorrell   +8 more
wiley   +1 more source

Proteomic characterization of plasmid pLA1 for biodegradation of polycyclic aromatic hydrocarbons in the marine bacterium, Novosphingobium pentaromativorans US6-1. [PDF]

PLoS ONE, 2014
Novosphingobium pentaromativorans US6-1 is a halophilic marine bacterium able to degrade polycyclic aromatic hydrocarbons (PAHs). Genome sequence analysis revealed that the large plasmid pLA1 present in N. pentaromativorans US6-1 consists of 199 ORFs and
Sung Ho Yun, Chi-Won Choi, Sang-Yeop Lee, Yeol Gyun Lee, Joseph Kwon, Sun Hee Leem, Young Ho Chung, Hyung-Yeel Kahng, Sang Jin Kim, Kae Kyoung Kwon, Seung Il Kim   +10 more
doaj   +1 more source

Synthesis, X-ray Structures, Electronic Properties, and O\u3csub\u3e2\u3c/sub\u3e/NO Reactivities of Thiol Dioxygenase Active-Site Models [PDF]

, 2016
Mononuclear non-heme iron complexes that serve as structural and functional mimics of the thiol dioxygenases (TDOs), cysteine dioxygenase (CDO) and cysteamine dioxygenase (ADO), have been prepared and characterized with crystallographic, spectroscopic ...
Brunold, Thomas C., Fiedler, Adam T., Fischer, Anne A., Lindeman, Sergey V., Stracey, Nuru   +4 more
core   +1 more source

Expression of Tryptophan 2,3-Dioxygenase in Metastatic Uveal Melanoma [PDF]

, 2020
Uveal melanoma (UM) is the most common primary eye malignancy in adults and up to 50% of patients subsequently develop systemic metastasis. Metastatic uveal melanoma (MUM) is highly resistant to immunotherapy.
Bhushan, Alok, Kaushal, Gagan, Lam, Bao, Link, Emma, Londin, Eric R, Orloff, Marlana, Rochani, Ankit, Sato, Takami, Terai, Mizue   +8 more
core   +1 more source

Analysis of acid-stressed Bacillus cereus reveals a major oxidative response and inactivation-associated radical formation [PDF]

, 2010
Acid stress resistance of the food-borne human pathogen Bacillus cereus may contribute to its survival in acidic environments, such as encountered in soil, food and the human gastrointestinal tract. The acid stress responses of B.
Abee, T., Kranenburg, R., van, Melis, C.C.J., van, Moezelaar, R., Mols, J.M.   +4 more
core   +2 more sources

Differential Phase Contrast Imaging to Predict MSC Immune Function

Advanced Healthcare Materials, EarlyView.
This work employs quantitative differential phase contrast (qDPC) imaging technique to non‐invasively extract morphological features from live Mesenchymal Stromal Cells (MSCs) for single cell prediction on cell immunomodulatory capacity. This work establishes a foundation for scalable, non‐destructive monitoring of MSCs immunomodulatory capacity ...
Kejie Rui, Priyanka Priyadarshani, Adrian Ross Liversage, Carlos Munoz, Steven Stice, Luke J. Mortensen   +5 more
wiley   +1 more source

Simultaneous 3-/4-Hydroxybenzoates Biodegradation and Arsenite Oxidation by Hydrogenophaga sp. H7

Frontiers in Microbiology, 2019
Aromatic compounds and arsenic (As) often coexist in the environment. As(III)-oxidizing bacteria can oxidize the more toxic As(III) into the less toxic As(V), and As(V) is easily removed.
Xia Fan, Li Nie, Kaixiang Shi, Qian Wang, Xian Xia, Gejiao Wang   +5 more
doaj   +1 more source