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Practical Direct Immunofluorescence
The American Journal of Dermatopathology, 2020Abstract: Direct immunofluorescence (DIF) remains a valuable tool that may be underused because of perceived challenges in the interpretation, limitations, and processing of DIF specimens. The aim of this review is to provide a practical guide for appropriately incorporating DIF in a variety of clinical diseases, such as autoimmune blistering
Nooshin Brinster, Randie H. Kim
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Direct immunofluorescence in pyoderma gangrenosum
British Journal of Dermatology, 1983Direct immunofluorescence was done in fifty-one cases of pyoderma gangrenosum. Biopsy specimens were taken from the peripheral erythematous zone of the lesion. In thirty-one cases (61%), there was positive immunofluorescence, with perivascular deposition of immune reactants being the most frequent pattern (twenty-seven cases).
F C, Powell +3 more
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Optimizing Direct Immunofluorescence
2014Immunofluorescence is a laboratory technique that utilizes a fluorophore-labeled antibody to detect immune complexes in tissue. Most of the labeled antibodies used in a clinical laboratory bind the conserved domains within each class of human antibodies, allowing them to detect a wide range of autoimmune complexes.
Ian D. Odell, Deborah L. Cook
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Direct Immunofluorescence in Sural Nerve Biopsies
European Neurology, 1988Direct immunofluorescence (DIF) has been carried out in 66 sural nerve biopsies using antibodies against human IgG, IgA, IgM, C3, C4, albumin, fibrinogen, and kappa- and lambda-chains. In 37 out of 63 (59%) different neuropathies immunoglobulins or other plasma proteins were found within the peripheral nerves. IgM along the myelin sheaths were found in
SCHENONE, ANGELO +6 more
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Granuloma annulare: direct immunofluorescence study
British Journal of Dermatology, 1976Direct immunofluorescence studies were carried out in eleven specimens of granuloma annulare. The results (in all cases) indicate the presence of focal deposition of fibrin, localized primarily in the intervascular portion of the dermis, corresponding to the granulomatous and necrobiotic areas. These findings implicate the cellular mechanism of delayed
Pablo Umbert, Richard K. Winkelmann
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Direct immunofluorescence in oral lichen planus
Oral Surgery, Oral Medicine, Oral Pathology, 1982Direct immunofluorescent staining (DIF) was performed on biopsy specimens from thirty-five patients with oral lichen planus. The results showed fibrin deposition in all cases at the mucosal-submucosal interface, within colloid bodies (fourteen of thirty-five) and within vascular walls (five of thirty-five).
Alexandra Sklavounou +2 more
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Direct Immunofluorescence in the Diagnosis of Legionnaires' Disease
Chest, 1981Over a 2 1/2 year period, 61 clinical specimens from 41 patients with pneumonia of uncertain etiology were evaluated for the presence of Legionella pneumophila (serogroups 1 to 4) by immunofluorescent antibody techniques. In 13 of 19 patients with Legionnaires' disease, the diagnosis was established by fluorescent antibody (FA) staining of lung ...
Louis Saravolatz +3 more
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Candidiasis visualised by proteinase-directed immunofluorescence
Virchows Archiv A Pathological Anatomy and Histopathology, 1991The secretory aspartic proteinases of Candida albicans and C. tropicalis are potential factors for virulence produced during infection. By indirect immunofluorescence, we have demonstrated proteinase antigen on elements of both species in deparaffinized tissue sections derived from clinical cases of mucosal and deep-seated candidiasis. Occasionally, we
U. Helmchen +3 more
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