A Direct Method for RT-PCR Detection of SARS-CoV-2 in Clinical Samples. [PDF]
El-Kafrawy SA +6 more
europepmc +1 more source
The Direct Method was the outcome of a reaction against the Grammar Translation Method. It was based on the assumption that the learner of a foreign language should think directly in the target language. According to this method, English is taught through English.
openaire +1 more source
The human gut microbiome across the life course
Despite significant individual variation and continuous change throughout life, the human gut microbiome follows some life stage‐specific trends. This article provides a brief overview of how gut microbiome composition shifts across different phases of life. Created in BioRender. Özkurt, E. (2026) https://BioRender.com/8q4nrnc.
Alise J. Ponsero +4 more
wiley +1 more source
Methodology for Lost Gas Determination from Exploratory Coal Cores and Comparative Evaluation of the Accuracy of the Direct Method. [PDF]
Xu H +8 more
europepmc +1 more source
Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto +3 more
wiley +1 more source
Direct method of extracting broadband complex refractive index from spectrophotometric measurements: an application to polydimethylsiloxane for passive radiative cooling. [PDF]
Czapla B, Hanssen L.
europepmc +1 more source
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Marginal adaptation of three-unit interim restorations fabricated by the CAD-CA systems and the direct method before and after thermocycling. [PDF]
Sadighpour L +3 more
europepmc +1 more source
From mice to humans—divergent strategies for intestinal homeostasis and regeneration
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa +2 more
wiley +1 more source
Generalized Hyers–Ulam stability of mixed-type additive-quartic mappings in 2-Banach spaces
This paper aims to explore the stability of a mixed-type additive-quartic functional equation in 2-Banach spaces via the direct method. We categorize mappings satisfying a certain functional inequality into odd, even, and general mappings, and establish ...
Ponmana Selvan Arumugam +2 more
doaj +1 more source

