Results 291 to 300 of about 3,674,005 (341)

Direct Dideoxy DNA Sequencing

2003
Advances in DNA sequencing techniques have made it possible to routinely sequence long DNA molecules (1,2). Recently double-stranded DNA has been directly sequenced with chain terminators (3), and this eliminates the tedious task of subcloning the fragments into single-stranded M13 vectors.
A, Graham, J, Steven, D, McKechnie
openaire   +2 more sources

Direct Sequencing of PCR Products

2003
Sequencing is an increasingly important part of the analysis of a polymerase chain reaction (PCR) product and is used in many areas of hemostasis. The protocols described in this chapter have been used to sequence many different PCR templates and provide high-quality, reproducible sequence data.
J C, Harwood, G A, Phear
openaire   +5 more sources

Direct Haplotype‐Specific DNA Sequencing

Preparative Biochemistry and Biotechnology, 2006
Determining haplotype-specific DNA sequence information is very important in a wide range of research fields. However, no simple and robust approaches are currently available for determining haplotype-specific sequence information. We have addressed this problem by developing a very simple and robust haplotype-specific sequencing approach.
Heidi, Rudi, Knut-Erik, Gylder, Odd, Arne Rognli, Knut, Rudi   +3 more
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Specific-primer-directed DNA sequencing

Analytical Biochemistry, 1986
A simple and rapid strategy for DNA sequence analysis based on the Sanger chain-termination method is described. This procedure utilizes full-sized inserts of 1 to 4 kb of DNA cloned into M13 bacteriophage vectors. After the sequence of the first 600-650 bp of the insert DNA has been determined with the commercially available universal vector primer, a
E C, Strauss, J A, Kobori, G, Siu, L E, Hood   +3 more
openaire   +2 more sources

Sequence Directed Hybrid Fuzzing

2020 IEEE 27th International Conference on Software Analysis, Evolution and Reengineering (SANER), 2020
Existing directed grey-box fuzzers are effective compared with coverage-based fuzzers. However, they fail to achieve a balance between effectiveness and efficiency, and it is difficult to cover complex paths due to random mutation. To mitigate the issue, we propose a novel approach, sequence directed hybrid fuzzing (SDHF), which leverages a sequence ...
Hongliang Liang, Lin Jiang, Lu Ai, Jinyi Wei   +3 more
openaire   +1 more source

Sequence-Directed Curvature Of Dna

Annual Review of Biochemistry, 1986
DNAs from both prokaryotic and eukaryotic organisms have yielded restriction fragments which manifest markedly anomalous electrophoretic behaviour (reduced mobility) when run on polyacrylamide gels. We have shown previously that the abnormal electrophoretic behaviour of one such fragment is a consequence of stable curvature of the helix axis in ...
openaire   +3 more sources

Direct Sequencing of PCR-Amplified DNA

Molecular Biotechnology, 1994
This article describes the direct sequencing of PCR-amplified DNA, a technique that bypasses the problem of replication errors sometimes associated with other PCR procedures. The direct sequencing procedure produces an "average sequence" of all the copies of the target. Any miscopied molecule usually represents only a small proportion of the total. The
P M, Green, F, Giannelli
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Reading sequence-directed computational nucleosome maps

Journal of Biomolecular Structure and Dynamics, 2014
Recently developed latest version of the sequence-directed single-base resolution nucleosome mapping reveals existence of strong nucleosomes and chromatin columnar structures (columns). Broad application of this simple technique for further studies of chromatin and chromosome structure requires some basic understanding as to how it works and what ...
Reshma, Nibhani, Edward N, Trifonov
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Direct-Sequence Systems

2011
A spread-spectrum signal is a signal with extra modulation that expands the signal bandwidth greatly beyond what is required by the underlying channel code and modulation. Spread-spectrum communication systems are useful for suppressing interference, making secure communications difficult to detect and process, accommodating fading and multipath ...
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