Results 131 to 140 of about 286,859 (297)

Evolution of Directed Triangle Motifs in the Google+ OSN [PDF]

, 2015
Motifs are a fundamental building block and distinguishing feature of networks. While characteristic motif distribution have been found in many networks, very little is known today about the evolution of network motifs.
Feldmann, A. ; https://orcid.org/, Uhlig, S, Feldmann, A, Uhlig, S., Doris Schiöberg, Fabian Schneider, Steve Uhlig, Schmid, S, Schneider, F, Schiöberg, D., Anja Feldmann, Stefan Schmid, Schmid, S., Schiöberg, D, Schneider, F.   +13 more
core  

Directed evolution of TEAS.

, 2014
(a) Procedure for the directed evolution of TEAS for higher cellular activity. (b) The carotenoid production level of E. coli cells harboring TEAS variants and pAC-MN-idi. (c) E. coli production of 5EA by TEAS variants.
Maiko Furubayashi (544674), Akira Fujii (544678), Jun Kajiwara (544676), Miki Iwasaki (544677), Ling Li (38566), Mayu Ikezumi (544675), Daisuke Umeno (544680), Kyoichi Saito (544679)   +7 more
core   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

LatentDE: latent-based directed evolution for protein sequence design

Machine Learning: Science and Technology
Directed evolution (DE) has been the most effective method for protein engineering that optimizes biological functionalities through a resource-intensive process of screening or selecting among a vast range of mutations.
Thanh V T Tran, Nhat Khang Ngo, Viet Thanh Duy Nguyen, Truong-Son Hy   +3 more
doaj   +1 more source

Navigating directed evolution efficiently: optimizing selection conditions and selection output analysis

Frontiers in Molecular Biosciences
Directed evolution is a powerful tool that can bypass gaps in our understanding of the sequence-function relationship of proteins and still isolate variants with desired activities, properties, and substrate specificities.
Paola Handal-Marquez, Hoai Nguyen, Vitor B. Pinheiro   +2 more
doaj   +1 more source

PARK(ing) time–How park deficiency affects the biological clock in a Drosophila model of Parkinson's disease

FEBS Letters, EarlyView.
Drosophila park mutants serve as a model for Parkinson's disease. We used this strain to investigate the connection between oxidative stress and the circadian clock mechanism. We showed that increased oxidative stress affects the physiology of pacemaker cells, disrupting their daily structural plasticity. Lack of rhythmic signaling from pacemaker cells
Kamila Zientara, Kinga Skoczek, Elzbieta Pyza, Milena Damulewicz   +3 more
wiley   +1 more source

Directed Evolution – A New Metaheuristc for Optimization

, 2017
Recently, we have witnessed an infusion of calculating models based on models offered by nature, models with more or less fidelity to the original that have led to the development of various problem-solving computational procedures.
Corina Rotar, Iantovics, L. B., Rotar, C., Laszlo Barna Iantovics   +3 more
core   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

Droplet Microfluidics for High-Throughput Screening and Directed Evolution of Biomolecules

Micromachines
Directed evolution is a powerful technique for creating biomolecules such as proteins and nucleic acids with tailor-made properties for therapeutic and industrial applications by mimicking the natural evolution processes in the laboratory.
Goran T. Vladisaljević
doaj   +1 more source

α‐Synuclein aggregation landscape from phase separation to neurotoxic intermediates

FEBS Letters, EarlyView.
Alpha‐synuclein aggregation in Parkinson's disease involves a complex landscape of transient intermediates, including oligomers, fibrils and liquid–liquid phase separation (LLPS). A view is emerging in which LLPS maturation into solid‐like condensates may contribute to the formation of neurotoxic species.
Silvia Arino, Giuliana Fusco, Alfonso De Simone   +2 more
wiley   +1 more source