Results 131 to 140 of about 269,742 (193)

Using MD Simulations to Understand the Impact of Directed Evolution on Oxygen Affinity in Amine Oxidases. [PDF]

ChemistryOpen
Field CR, Lindeque R, Dolan JP, Østerby Madsen J, Hay S, Turner NJ, Woodley JM, Woodley JM, Cosgrove SC.   +8 more
europepmc   +1 more source

Tumor B‐cell infiltration in platinum‐treated advanced muscle‐invasive urothelial carcinoma

Molecular Oncology, EarlyView.
Bladder tumors with higher pretreatment memory B‐cell infiltration were linked to longer survival after cisplatin chemotherapy, but not carboplatin. These tumors also showed more organized immune structures (tertiary lymphoid structures) and a shared pro‐inflammatory B‐cell‐rich community, suggesting that memory B cells may help identify patients most ...
Konrad Stawiski, Júlia Perera‐Bel, Alejo Rodriguez‐Vida, Nuria Juanpere, Jihyun Lee, Daniel E. Michaud, Jennifer L. Guerriero, Kent W. Mouw, Aristotelis Bamias, Filipe L. F. Carvalho, Joaquim Bellmunt   +10 more
wiley   +1 more source

Correction to 'Directed evolution of hyperactive integrases for site specific insertion of transgenes'. [PDF]

Nucleic Acids Res
europepmc   +1 more source

Directed evolution of Clec12a-binding peptides using mammalian-expressed recombinant protein. [PDF]

Biochem Biophys Rep
Kawaguchi Y, Islam MS, Yokoyama M, Hosokawa T, Nakaya M, Kamiya N, Goto M.   +6 more
europepmc   +1 more source

Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis

Molecular Oncology, EarlyView.
This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan   +3 more
wiley   +1 more source

Computational redesign and directed evolution of a lanthanide-dependent photoredox enzyme for enantioselective diol cleavage. [PDF]

Chem Sci
Leiss-Maier F, Behringer J, Mustafa G, Heider A, Mühlhofer R, Klein AS, Groll M, Zeymer C.   +7 more
europepmc   +1 more source

Epigenetic heterogeneity and plasticity in therapy‐induced tumor states through single‐cell multi‐omics

Molecular Oncology, EarlyView.
Single‐cell multi‐omics reveals epigenetic heterogeneity across therapy‐adaptive tumor states, including quiescent/dormant, drug‐tolerant persister, and EMT‐like phenotypes. By linking regulatory features with state‐associated biomarkers, these approaches inform biomarker‐guided therapeutic strategies for evolving tumors.
Hee Jung Kim, Hwiyeong Lee, Jin Hong, Daechan Park   +3 more
wiley   +1 more source

Directed evolution of a beta-lactamase samples a wide variety of conformational states. [PDF]

Protein Sci
Sun J, Timmer M, Brünle S, Boyle AL, Ubbink M.   +4 more
europepmc   +1 more source

PAK1 activation drives divergent resistance mechanisms to aromatase inhibition and tamoxifen in a luminal: A breast cancer model

Molecular Oncology, EarlyView.
Breast cancer remains a major cause of cancer death in women, frequently developing endocrine therapy resistance. This study demonstrates that upregulated p21‐activated kinase 1 (PAK1) activity drives resistance to tamoxifen and long‐term estrogen deprivation in ER+ breast cancer models.
Luisa Schwarzmüller, Janina Müller, Efstathios‐Iason Vlachavas, Lukas Beumers, Lena Fleischhacker, Sara Burmester, Angelika Wörner, Sabine Karolus, Dominic Helm, Cindy Körner, Stefan Wiemann   +10 more
wiley   +1 more source

Harnessing mass spectrometry-based proteomics for continuous directed evolution. [PDF]

Synth Biol (Oxf)
Belt K, Obe D, Wilson MA, Millar AH, Bathe U.   +4 more
europepmc   +1 more source