Results 111 to 120 of about 11,830,288 (332)

Directed evolution of nucleotide-based libraries using lambda exonuclease

BioTechniques, 2012
Directed evolution of nucleotide libraries using recombination or mutagenesis is an important technique for customizing catalytic or biophysical traits of proteins.
Bee Nar Lim, Yee Siew Choong, Asma Ismail, Jörn Glökler, Zoltán Konthur, Theam Soon Lim   +5 more
doaj   +1 more source

CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors

Genome Biology, 2019
Increasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based directed evolution platform in plants to evolve the rice (Oryza sativa) SF3B1 spliceosomal ...
Haroon Butt, Ayman Eid, A. Momin, J. Bazin, M. Crespi, S. Arold, Magdy M. Mahfouz   +6 more
semanticscholar   +1 more source

Initial mutations direct alternative pathways of protein evolution [PDF]

, 2011
Whether evolution is erratic due to random historical details, or is repeatedly directed along similar paths by certain constraints, remains unclear. Epistasis (i.e.
J Arjan G M de Visser, Tawfik Dan S., Salverda, M.L.M., Merijn L M Salverda, Hoekstra, R.F., Salverda Merijn L. M., Dellus, E., John van der Oost, Alfons J M Debets, Visser, J.A.G.M., de, Gorter Florien A., de Visser J. Arjan G. M., Florien A Gorter, Dellus Eynat, Dan S Tawfik, Gorter, F.A., Debets Alfons J. M., van der Oost John, Eynat Dellus, Hoekstra Rolf F., Debets, A.J.M., Oost, J., van der, J. Arjan G. M. de Visser, Merijn L. M. Salverda, Tawfik, D.S., Florien A. Gorter, Alfons J. M. Debets, Rolf F Hoekstra, Rolf F. Hoekstra, Dan S. Tawfik   +29 more
core   +2 more sources

Chemotherapy for Spinal Cord Intramedullary Pediatric Low‐Grade Glioma—A Systematic Literature Review

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Pediatric intramedullary low‐grade gliomas (PIMLGGs) are rare neoplasms that present unique clinical and management challenges. Although surgery remains the primary tool for tissue diagnosis and decompression, complete gross‐total resection is frequently unfeasible due to the infiltrative nature of these lesions.
Olga M. Sergeenko, Segev Gabay, Jonathan Roth, Amit Ringel, Rina Dvir, Shlomi Constantini   +5 more
wiley   +1 more source

“We Cannot Talk as a Family”: Complexities of Survivorship Communication Among Hispanic/Latino Young Adult Survivors of Childhood Cancer, Parents, and Clinicians

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Young adult childhood cancer survivors (YA‐CCS) have an increased risk of treatment‐related health complications throughout their lives. Lifelong survivorship care is recommended, yet many YA‐CCS experience disruptions in care during pediatric‐to‐adult transitions and do not receive recommended survivorship care.
Kevin Shanchen Pu, Nicole Fernández‐Viña, Briana C. Martin‐Villa, Daniela Ramirez, Rebecca M. Lewinsohn, Mary Smith, Heidi Boynton, Lidia Schapira, Stephanie M. Smith   +8 more
wiley   +1 more source

Fluorescence Activated Droplet Sorting for Single Cell Directed Evolution

ACS Synthetic Biology, 2019
Synthetic biology aims to improve human health and the environment by repurposing biological enzymes for use in practical applications. However, natural enzymes often function with suboptimal activity when engineered into biological pathways or ...
Derek Vallejo, Ali Nikoomanzar, B. M. Paegel, J. Chaput   +3 more
semanticscholar   +1 more source

Self-Directed Learning in Italy: Problems and Possibilities

, 2008
This article presents the evolution of the idea of self-directed learning in Italy through three major phases. At the end of the twentieth century, self-directed learning became identified with techniques of self-instruction for adults unable to ...
BIASIN, CHIARA
core  

Classical Complement Pathway Inhibition in a Pediatric Patient With Refractory Immune Thrombocytopenia

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Pediatric refractory immune thrombocytopenia (rITP) is generally characterized by persistent thrombocytopenia and failure to achieve a sustained response to multiple standard therapies. We report a 4‐year‐old female with congenital tufting enteropathy, post‐intestinal transplant patient on immunosuppressants, who developed severe rITP ...
Sarah Hsieh, Susmita Sarangi, Caileigh Pudela, Catherine M. Broome, Tara K. Suntum   +4 more
wiley   +1 more source

Returnability in complex directed networks (digraphs) [PDF]

, 2009
The concept of returnability is proposed for complex directed networks (digraphs). It can be seen as a generalization of the concept of reciprocity. Two measures of the returnability are introduced. We establish closed formulas for the calculation of the
Estrada, Ernesto, Hatano, Naomichi
core   +1 more source

Directed Evolution of Bicyclic Peptides for Therapeutic Application

CHIMIA, 2013
Many naturally occurring cyclic peptides or derivatives thereof are used as therapeutics such as the human hormones vasopressin and oxytocin or the antibiotics vancomycin and daptomycin.
Philippe Diderich, Christian Heinis
doaj   +1 more source