Results 101 to 110 of about 286,859 (297)
Synthetic Gene Circuits: Design with Directed Evolution [PDF]
, 2007 Synthetic circuits offer great promise for generating insights into nature's underlying design principles or forward engineering novel biotechnology applications. However, construction of these circuits is not straightforward.
Haseltine, Eric L., Arnold, Frances H.
core
Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system
FEBS Letters, EarlyView.Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley +1 more source
Exploring protein fitness landscapes by directed evolution [PDF]
, 2009 Directed evolution circumvents our profound ignorance of how a protein's sequence encodes its function by using iterative rounds of random mutation and artificial selection to discover new and useful proteins.
Arnold, Frances H., Romero, Philip A.
core
The directed evolution trials.
, 2015 (A) Scheme for directed evolution of RecA variants with improved functionality in conjugational recombination. (B) Genetic markers used in the conjugation trials.
Sindhu Chitteni-Pattu (750595) +5 more
core +1 more source
Diversity and complexity in neural organoids
FEBS Letters, EarlyView.Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley +1 more source
, 2001 This paper is an overview of the philosophy of evolution past, present, and future to be published in Blackwell's Guide to the Philosophy of Science, edited by P.K. Machamer and M. Silberstein. It surveys the following topics: the neutralist/selectionist
Millstein, Roberta L.
core
Protein Engineering and Directed Evolution for Nanocarrier Innovation
CHIMIAThe design of nonviral protein-based delivery systems has gained significant attention as an alternative to viral vectors and lipid nanoparticles (LNPs) for gene therapy.
Angela Steinauer
doaj +1 more source
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source
The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes
FEBS Letters, EarlyView.Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga +3 more
wiley +1 more source
Different Approaches For Protein Engineering In Industrial Biotechnology
, 2011 Protein engineering is the novel field which has wide applications from pharmaceutics, industry, commercial, laundry and research. It may apply rational design or non rational design or both.
Muhammad Waheed Akhtar, Israr Khan
core