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Rapid directed molecular evolution of fluorescent proteins in mammalian cells. [PDF]
Protein Sci, 2022 Abstract In vivo imaging of model organisms is heavily reliant on fluorescent proteins with high intracellular brightness. Here we describe a practical method for rapid optimization of fluorescent proteins via directed molecular evolution in cultured mammalian cells.
Babakhanova S +18 more
europepmc +7 more sources
Directed Evolution in Drops: Molecular Aspects and Applications [PDF]
ACS Synthetic Biology, 2021 The process of optimizing the properties of biological molecules is paramount for many industrial and medical applications. Directed evolution is a powerful technique for modifying and improving biomolecules such as proteins or nucleic acids (DNA or RNA).
Aitor Manteca +2 more
exaly +5 more sources
A directed molecular evolution approach to improved immunogenicity of the HIV-1 envelope glycoprotein. [PDF]
PLoS ONE, 2011 A prophylactic vaccine is needed to slow the spread of HIV-1 infection. Optimization of the wild-type envelope glycoproteins to create immunogens that can elicit effective neutralizing antibodies is a high priority.
Sean X Du +14 more
doaj +5 more sources
Combinatorial Avidity Selection of Mosaic Landscape Phages Targeted at Breast Cancer Cells—An Alternative Mechanism of Directed Molecular Evolution [PDF]
Viruses, 2019 Low performance of actively targeted nanomedicines required revision of the traditional drug targeting paradigm and stimulated the development of novel phage-programmed, self-navigating drug delivery vehicles.
Valery A. Petrenko +4 more
doaj +2 more sources
Directed molecular evolution to design advanced red fluorescent proteins. [PDF]
Nat Methods, 2011 Fluorescent proteins have become indispensable imaging tools for biomedical research. Continuing progress in fluorescence imaging, however, requires probes with additional colors and properties optimized for emerging techniques. Here we summarize strategies for development of red-shifted fluorescent proteins.
Subach FV, Piatkevich KD, Verkhusha VV.
europepmc +4 more sources
Discovery of Superior Enzymes by Directed Molecular Evolution
ChemBioChem, 2001 Natural selection has created optimal catalysts that exhibit their convincing performance even with a number of sometimes counteracting constraints. Optimal performance of enzyme catalysis does not refer necessarily to maximum reaction rate. Rather, it may involve a compromise between specificity, rate, stability, and other chemical constraints ; in ...
Susanne Brakmann
exaly +5 more sources
Food Technology and Biotechnology, 2018 L-Cysteine sulfinate decarboxylase (CSD, EC 4.1.1.29), the rate-limiting enzyme in taurine synthesis pathway, catalyzes L-cysteine sulfinic acid to form hypotaurine.
Jie Deng +6 more
doaj +3 more sources
Frontiers in Microbiology, 2021 Some organisms have shown the ability to naturally survive in extreme environments, even outer space. Some of these have natural mechanisms to resist severe DNA damage from conditions such as ionizing and non-ionizing radiation, extreme temperatures, and
Jaume Puig +11 more
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Directed molecular evolution by somatic hypermutation [PDF]
Protein Engineering Design and Selection, 2004 After rearrangement of immunoglobulin gene segments, the immune system evolves the antibody repertoire by mutating the immunoglobulin variable region at a high rate. While this somatic hypermutation was thought to occur only at the variable region, recent studies suggest that hypermutation can occur at locations throughout the genome.
Clifford L, Wang +2 more
openaire +2 more sources
Optimal Evolutionary Control for Artificial Selection on Molecular Phenotypes
Physical Review X, 2021 Controlling an evolving population is an important task in modern molecular genetics, including directed evolution for improving the activity of molecules and enzymes, in breeding experiments in animals and in plants, and in devising public health ...
Armita Nourmohammad, Ceyhun Eksin
doaj +1 more source