Results 1 to 10 of about 17,272 (247)

Stepwise error‐prone PCR and DNA shuffling changed the pH activity range and product specificity of the cyclodextrin glucanotransferase from an alkaliphilicBacillus sp [PDF]

FEBS Open Bio, 2015
Cyclodextrin glucanotransferase (EC 2.4.1.19) from the alkaliphilicBacillus sp. G‐825‐6 converts starch mainly to γ‐cyclodextrin (CD8). A combination of error‐prone PCR and DNA shuffling was used to obtain variants of this enzyme with higher product ...
Susanne Melzer, Christian Sonnendecker, Christina Föllner, Wolfgang Zimmermann   +3 more
doaj   +3 more sources

Characterization of Protein Domain Function via in vitro DNA Shuffling [PDF]

Bio-Protocol, 2018
We recently investigated the molecular events that drive evolution of the CTX-M-type β-lactamases by DNA shuffling of fragments of the blaCTX-M-14 and blaCTX-M-15 genes.
Kathy Po, Edward Chan, Sheng Chen
doaj   +2 more sources

Directed DNA shuffling of retrovirus and retrotransposon integrase protein domains. [PDF]

PLoS ONE, 2013
Chimeric proteins are used to study protein domain functions and to recombine protein domains for novel or optimal functions. We used a library of chimeric integrase proteins to study DNA integration specificity.
Xiaojie Qi, Edwin Vargas, Liza Larsen, Whitney Knapp, G Wesley Hatfield, Richard Lathrop, Suzanne Sandmeyer   +6 more
doaj   +2 more sources

DNA shuffling to improve crude-water interfacial activity in biosurfactants with OmpA protein of Escherichia coli [PDF]

PeerJ
Surfactants are molecules derived primarily from petroleum that can reduce the surface tension at interfaces. Their slow degradation is a characteristic that could cause environmental issues.
Vanessa Lucía Nuñez Velez, Liseth Daniela Villamizar Gomez, Jhon E. Mendoza Ospina, Yasser Hayek-Orduz, Miguel Fernandez-Niño, Silvia Restrepo Restrepo, Óscar Alberto Álvarez Solano, Luis H. Reyes Barrios, Andres F. Gonzalez Barrios   +8 more
doaj   +3 more sources

A novel chimeric avidin with increased thermal stability using DNA shuffling. [PDF]

PLoS ONE, 2014
Avidins are a family of proteins widely employed in biotechnology. We have previously shown that functional chimeric mutant proteins can be created from avidin and avidin-related protein 2 using a methodology combining random mutagenesis by recombination
Barbara Taskinen, Tomi T Airenne, Janne Jänis, Rolle Rahikainen, Mark S Johnson, Markku S Kulomaa, Vesa P Hytönen   +6 more
doaj   +2 more sources

Toxic Activity, Molecular Modeling and Docking Simulations of Bacillus thuringiensis Cry11 Toxin Variants Obtained via DNA Shuffling [PDF]

Frontiers in Microbiology, 2018
The Cry11 family belongs to a large group of δ-endotoxins that share three distinct structural domains. Among the dipteran-active toxins referred to as three-domain Cry11 toxins, the Cry11Aa protein from Bacillus thuringiensis subsp.
Alvaro Mauricio Florez, Miguel Orlando Suarez-Barrera, Miguel Orlando Suarez-Barrera, Gloria M. Morales, Karen Viviana Rivera, Sergio Orduz, Rodrigo Ochoa, Diego Guerra, Carlos Muskus   +8 more
doaj   +2 more sources

Expanding the Plant GSTome Through Directed Evolution: DNA Shuffling for the Generation of New Synthetic Enzymes With Engineered Catalytic and Binding Properties [PDF]

Frontiers in Plant Science, 2018
Glutathione transferases (GSTs, EC. 2.5.1.18) are inducible multifunctional enzymes that are essential in the detoxification and degradation of toxic compounds. GSTs have considerable biotechnological potential.
Evangelia G. Chronopoulou, Anastassios C. Papageorgiou, Farid Ataya, Irini Nianiou-Obeidat, Panagiotis Madesis, Nikolaos E. Labrou   +5 more
doaj   +2 more sources

Codon-Optimization of Wild-Type Adeno-Associated Virus Capsid Sequences Enhances DNA Family Shuffling while Conserving Functionality

Molecular Therapy: Methods & Clinical Development, 2019
Adeno-associated virus (AAV) vectors have become one of the most widely used gene transfer tools in human gene therapy. Considerable effort is currently being focused on AAV capsid engineering strategies with the aim of developing novel variants with ...
Marti Cabanes-Creus, Samantha L. Ginn, Anais K. Amaya, Sophia H.Y. Liao, Adrian Westhaus, Claus V. Hallwirth, Patrick Wilmott, Jason Ward, Kimberley L. Dilworth, Giorgia Santilli, Arkadiusz Rybicki, Hiroyuki Nakai, Adrian J. Thrasher, Adrian C. Filip, Ian E. Alexander, Leszek Lisowski   +15 more
doaj   +3 more sources

Algorithms for optimizing cross-overs in DNA shuffling. [PDF]

BMC Bioinformatics, 2012
Abstract Background DNA shuffling generates combinatorial libraries of chimeric genes by stochastically recombining parent genes. The resulting libraries are subjected to large-scale genetic selection or screening to identify those chimeras with favorable properties (e.g., enhanced stability or enzymatic activity ...
He L, Friedman AM, Bailey-Kellogg C.
europepmc   +4 more sources

Molecular Breeding of Genes, Pathways and Genomes by Dna Shuffling [PDF]

The Scientific World Journal, 2002
Existing methods for optimization of sequences by random mutagenesis generate libraries with a small number of mostly deleterious mutations, resulting in libraries containing a large fraction of non-functional clones that explore only a small part of squence space.
WIllem P.C. Stemmer
doaj   +4 more sources