Results 1 to 10 of about 286,859 (297)

Directed Evolution of a Mycobacteriophage [PDF]

Antibiotics, 2019
Bacteriophages represent an alternative strategy to combat pathogenic bacteria. Currently, Mycobacterium tuberculosis infections constitute a major public health problem due to extensive antibiotic resistance in some strains.
María Cebriá-Mendoza, Rafael Sanjuán, Pilar Domingo-Calap   +2 more
doaj   +5 more sources

Directed evolution: selection of the host organism

Computational and Structural Biotechnology Journal, 2012
Directed evolution has become a well-established tool for improving proteins and biological systems. A critical aspect of directed evolution is the selection of a suitable host organism for achieving functional expression of the target gene.
Azadeh Pourmir, Tyler W Johannes
doaj   +6 more sources

A chimeric viral platform for directed evolution in mammalian cells [PDF]

Nature Communications
Directed evolution is a process of mutation and artificial selection to breed biomolecules with new or improved activity. Directed evolution platforms are primarily prokaryotic or yeast-based, and stable mammalian systems have been challenging to ...
Alexander J. Cole, Christopher E. Denes, Cesar L. Moreno, Lise Hunault, Thomas Dobson, Daniel Hesselson, G. Gregory Neely   +6 more
doaj   +3 more sources

Directed Evolution and Biocatalysis [PDF]

Angewandte Chemie - International Edition, 2001
This review describes the current state of biocatalysis in the chemical industry. Although we recognize the advantages of chemical approaches, we suggest that the use of biological catalysis is about to expand dramatically because of the recent developments in the artificial evolution of genes that code for enzymes. For the first time it is possible to
Willem P C Stemmer
exaly   +3 more sources

Directed Evolution of Microbial Communities [PDF]

Annual Review of Biophysics, 2020
Directed evolution is a form of artificial selection that has been used for decades to find biomolecules and organisms with new or enhanced functional traits. Directed evolution can be conceptualized as a guided exploration of the genotype–phenotype map, where genetic variants with desirable phenotypes are first selected and then mutagenized to search ...
Rebolleda-Gomez, Maria, Chang, Chang-Yu, Estrela, Sylvie, Vila, Jean, Sanchez, Alvaro, Diaz-Colunga, Juan   +5 more
openaire   +7 more sources

Directed evolution of hydrocarbon-producing enzymes [PDF]

Biotechnology for Biofuels and Bioproducts
Enzymes capable of catalysing the production of hydrocarbons hold promise for sustainable fuel synthesis. However, the native activities of these enzymes are often insufficient for their exploitation in industrial bioprocesses.
Jochem R. Nielsen, Joseph Kennerley, Wei E. Huang   +2 more
doaj   +2 more sources

Directed evolution of drug-like Aβ conformation-specific antibodies [PDF]

Frontiers in Immunology
Monoclonal antibodies that recognize conformational epitopes in protein aggregates are important for research, diagnostic, and therapeutic applications related to neurodegenerative disorders such as Alzheimer’s and Parkinson’s diseases. Unfortunately, it
Alec A. Desai, Alec A. Desai, Matthew D. Smith, Matthew D. Smith, Jennifer M. Zupancic, Jennifer M. Zupancic, Emily K. Makowski, Emily K. Makowski, Yulei Zhang, Yulei Zhang, Julia E. Gerson, Shannon J. Moore, Shannon J. Moore, Alexandra B. Sutter, Sean P. Ferris, Magdalena I. Ivanova, Magdalena I. Ivanova, Magdalena I. Ivanova, Geoffrey G. Murphy, Geoffrey G. Murphy, Geoffrey G. Murphy, Henry L. Paulson, Henry L. Paulson, Henry L. Paulson, Henry L. Paulson, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier   +30 more
doaj   +2 more sources

CADEE: Computer-Aided Directed Evolution of Enzymes

IUCrJ, 2017
The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a ...
Beat Anton Amrein, Fabian Steffen-Munsberg, Ireneusz Szeler, Miha Purg, Yashraj Kulkarni, Shina Caroline Lynn Kamerlin   +5 more
doaj   +2 more sources

Active learning-assisted directed evolution [PDF]

Nature Communications
Directed evolution (DE) is a powerful tool to optimize protein fitness for a specific application. However, DE can be inefficient when mutations exhibit non-additive, or epistatic, behavior.
Jason Yang, Ravi G. Lal, James C. Bowden, Raul Astudillo, Mikhail A. Hameedi, Sukhvinder Kaur, Matthew Hill, Yisong Yue, Frances H. Arnold   +8 more
doaj   +2 more sources

Optimisation strategies for directed evolution without sequencing.

PLoS Computational Biology
Directed evolution can enable engineering of biological systems with minimal knowledge of their underlying sequence-to-function relationships. A typical directed evolution process consists of iterative rounds of mutagenesis and selection that are ...
Jessica James, Sebastian Towers, Jakob Foerster, Harrison Steel   +3 more
doaj   +2 more sources