Results 1 to 10 of about 1,378,774 (287)

Active learning-assisted directed evolution [PDF]

Nature Communications
Directed evolution (DE) is a powerful tool to optimize protein fitness for a specific application. However, DE can be inefficient when mutations exhibit non-additive, or epistatic, behavior.
Jason Yang, Ravi G. Lal, James C. Bowden, Raul Astudillo, Mikhail A. Hameedi, Sukhvinder Kaur, Matthew Hill, Yisong Yue, Frances H. Arnold   +8 more
doaj   +4 more sources

Directed evolution of hydrocarbon-producing enzymes [PDF]

Biotechnology for Biofuels and Bioproducts
Enzymes capable of catalysing the production of hydrocarbons hold promise for sustainable fuel synthesis. However, the native activities of these enzymes are often insufficient for their exploitation in industrial bioprocesses.
Jochem R. Nielsen, Joseph Kennerley, Wei E. Huang   +2 more
doaj   +2 more sources

Directed evolution of drug-like Aβ conformation-specific antibodies [PDF]

Frontiers in Immunology
Monoclonal antibodies that recognize conformational epitopes in protein aggregates are important for research, diagnostic, and therapeutic applications related to neurodegenerative disorders such as Alzheimer’s and Parkinson’s diseases. Unfortunately, it
Alec A. Desai, Alec A. Desai, Matthew D. Smith, Matthew D. Smith, Jennifer M. Zupancic, Jennifer M. Zupancic, Emily K. Makowski, Emily K. Makowski, Yulei Zhang, Yulei Zhang, Julia E. Gerson, Shannon J. Moore, Shannon J. Moore, Alexandra B. Sutter, Sean P. Ferris, Magdalena I. Ivanova, Magdalena I. Ivanova, Magdalena I. Ivanova, Geoffrey G. Murphy, Geoffrey G. Murphy, Geoffrey G. Murphy, Henry L. Paulson, Henry L. Paulson, Henry L. Paulson, Henry L. Paulson, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier   +30 more
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A chimeric viral platform for directed evolution in mammalian cells [PDF]

Nature Communications
Directed evolution is a process of mutation and artificial selection to breed biomolecules with new or improved activity. Directed evolution platforms are primarily prokaryotic or yeast-based, and stable mammalian systems have been challenging to ...
Alexander J. Cole, Christopher E. Denes, Cesar L. Moreno, Lise Hunault, Thomas Dobson, Daniel Hesselson, G. Gregory Neely   +6 more
doaj   +2 more sources

Optimisation strategies for directed evolution without sequencing. [PDF]

PLoS Computational Biology
Directed evolution can enable engineering of biological systems with minimal knowledge of their underlying sequence-to-function relationships. A typical directed evolution process consists of iterative rounds of mutagenesis and selection that are ...
Jessica James, Sebastian Towers, Jakob Foerster, Harrison Steel   +3 more
doaj   +2 more sources

A risk based assessment approach for chemical mixtures from wastewater treatment plant effluents

Environment International, 2022
In this study, 56 effluent samples from 52 European wastewater treatment plants (WWTPs) were investigated for the occurrence of 499 emerging chemicals (ECs) and their associated potential risks to the environment.
Saskia Finckh, Liza-Marie Beckers, Wibke Busch, Eric Carmona, Valeria Dulio, Lena Kramer, Martin Krauss, Leo Posthuma, Tobias Schulze, Jaap Slootweg, Peter C. Von der Ohe, Werner Brack   +11 more
doaj   +1 more source

Cybernetics and Directed Evolution

Кібернетика та комп'ютерні технології, 2023
Results. The place and role of cybernetics methods for solving the global problem of directed evolution are considered. The author investigates the eventual phenomenon of the interaction of two coryphaei of Ukrainian science V.I.
Oleksandr Palagin
doaj   +1 more source

Directed Evolution Methods for Enzyme Engineering

Molecules, 2021
Enzymes underpin the processes required for most biotransformations. However, natural enzymes are often not optimal for biotechnological uses and must be engineered for improved activity, specificity and stability.
Saurabh Rajendra Nirantar
doaj   +1 more source

RNA-mediated in vivo Directed Evolution in Yeast

Bio-Protocol, 2022
Directed evolution is a powerful approach to obtain genetically-encoded sought-for traits. Compared to the prolonged adaptation regimes to mutations occurring under natural selection, directed evolution unlocks rapid screening and selection of mutants ...
Emil Jensen, Michael Jensen
doaj   +1 more source

Models for the directed evolution of bacterial allelopathy: bacteriophage lysins [PDF]

PeerJ, 2015
Microbes produce a variety of compounds that are used to kill or suppress other species. Traditional antibiotics have their origins in these natural products, as do many types of compounds being pursued today in the quest for new antibacterial drugs ...
James J. Bull, Cameron Crandall, Anna Rodriguez, Stephen M. Krone   +3 more
doaj   +2 more sources