Results 1 to 10 of about 11,830,288 (332)

Directed evolution methods for overcoming trade-offs between protein activity and stability

AIChE journal. American Institute of Chemical Engineers, 2019
Engineered proteins are being widely developed and employed in applications ranging from enzyme catalysts to therapeutic antibodies. Directed evolution, an iterative experimental process composed of mutagenesis and library screening, is a powerful ...
Samuel D. Stimple, Matthew Smith, Peter M. Tessier   +2 more
semanticscholar   +1 more source

Protein Engineering and Directed Evolution for Nanocarrier Innovation

CHIMIA
The design of nonviral protein-based delivery systems has gained significant attention as an alternative to viral vectors and lipid nanoparticles (LNPs) for gene therapy.
Angela Steinauer
doaj   +1 more source

Rescue of conformational dynamics in enzyme catalysis by directed evolution

Nature Communications, 2018
A key challenge in the field of protein design and evolution is to understand the mechanisms by which directed evolution is improving enzymes. Here the authors combine different biophysical methods and give mechanistic insights into how directed ...
Renee Otten, Lin Liu, Lillian R. Kenner, Michael W. Clarkson, David Mavor, Dan S. Tawfik, Dorothee Kern, James S. Fraser   +7 more
doaj   +1 more source

Directed evolution of VanR biosensor specificity in yeast

Biotechnology Notes
Allosterically regulated transcription factors (aTFs) based biosensors from prokaryotes have been widely used to screen large gene libraries, stabilize engineered microbes from evolutionary drifting, and for detection of soil pollutants, among many other
Vasil D'Ambrosio, Subrata Pramanik, Kati Goroncy, Tadas Jakočiūnas, David Schönauer, Mehdi D. Davari, Ulrich Schwaneberg, Jay D. Keasling, Michael K. Jensen   +8 more
doaj   +1 more source

Directed Evolution with Fast and Efficient Selection Technologies

CHIMIA, 2001
Directed molecular evolution has proven to be a very powerful concept for the generation of proteins with improved properties, such as increased activity, binding affinity, folding efficiency or enhanced chemical and/or thermodynamic stability ...
Ekkehard Mössner, Andreas Plückthun
doaj  

LatentDE: latent-based directed evolution for protein sequence design

Machine Learning: Science and Technology
Directed evolution (DE) has been the most effective method for protein engineering that optimizes biological functionalities through a resource-intensive process of screening or selecting among a vast range of mutations.
Thanh V T Tran, Nhat Khang Ngo, Viet Thanh Duy Nguyen, Truong-Son Hy   +3 more
doaj   +1 more source

Interface-specific evolution in directed energy deposition-processed SS316L-CuSn10-NiTi joints

Scientific Reports
Directed Energy Deposition (DED) can join dissimilar alloys, but differences in thermophysical properties and interfacial chemistry make the process difficult.
Bakytzhan Sariyev, Yeldar Otynshiyev, Andas Amrin, Nikita Lutchenko, Boris Golman   +4 more
doaj   +1 more source

Navigating directed evolution efficiently: optimizing selection conditions and selection output analysis

Frontiers in Molecular Biosciences
Directed evolution is a powerful tool that can bypass gaps in our understanding of the sequence-function relationship of proteins and still isolate variants with desired activities, properties, and substrate specificities.
Paola Handal-Marquez, Hoai Nguyen, Vitor B. Pinheiro   +2 more
doaj   +1 more source

Directed evolution of adenine base editors with increased activity and therapeutic application

Nature Biotechnology, 2020
Nicole M. Gaudelli, Dieter K. Lam, H. Rees, Noris M. Solá-Esteves, Luis A. Barrera, D. Born, Aaron Edwards, Jason M. Gehrke, Seung-Joo Lee, A. Liquori, Ryan C. Murray, M. Packer, C. Rinaldi, I. Slaymaker, Jonathan S. Yen, Lauren E. Young, G. Ciaramella   +16 more
semanticscholar   +1 more source

In vitro generation of genetic diversity for directed evolution by error-prone artificial DNA synthesis

Communications Biology
Generating genetic diversity lies at the heart of directed evolution which has been widely used to engineer genetic parts and gene circuits in synthetic biology.
Baowei Wang, Yang Liu, Xuelian Bai, Huijuan Tian, Lina Wang, Miao Feng, Hairong Xia   +6 more
doaj   +1 more source