Results 31 to 40 of about 1,378,774 (287)
Laboratory-Directed Protein Evolution [PDF]
Microbiology and Molecular Biology Reviews, 2005 SUMMARYSystematic approaches to directed evolution of proteins have been documented since the 1970s. The ability to recruit new protein functions arises from the considerable substrate ambiguity of many proteins. The substrate ambiguity of a protein can be interpreted as the evolutionary potential that allows a protein to acquire new specificities ...
Ling, Yuan +3 more
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Directed evolution converts subtilisin E into a functional equivalent of thermitase [PDF]
, 1999 We used directed evolution to convert Bacillus subtilis subtilisin E into an enzyme functionally equivalent to its thermophilic homolog thermitase from Thermoactinomyces vulgaris.
Arnold, Frances H., Zhao, Huimin
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F1000Research, 2015 In prokaryotes, horizontal gene transfer (HGT) has been regarded as an important evolutionary drive to acquire and retain beneficial genes for their survival in diverse ecologies.
Punita Sharma +11 more
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PAIDDE: A Permutation-Archive Information Directed Differential Evolution Algorithm
IEEE Access, 2022 Evolutionary algorithms have shown great successes in various real-world applications ranging in molecule to astronomy. As a mainstream evolutionary algorithm, differential evolution (DE) possesses the characteristics of simple algorithmic structure ...
Xiaosi Li +5 more
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Directing evolution in cells [PDF]
Nature Methods, 2020 A CRISPR–Cas9-based approach generates libraries of mutant proteins targeted to specific subcellular compartments for in situ directed evolution.
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Directed percolation depinning models: Evolution equations
, 1999 We present the microscopic equation for the growing interface with quenched noise for the model first presented by Buldyrev et al. [Phys. Rev. A 45, R8313 (1992)]. The evolution equation for the height, the mean height, and the roughness are reached in a
A. Díaz-Sánchez +11 more
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Directed Random Market: the equilibrium distribution [PDF]
, 2014 We find the explicit expression for the equilibrium wealth distribution of the Directed Random Market process, recently introduced by Mart\'inez-Mart\'inez and L\'opez-Ruiz, which turns out to be a Gamma distribution with shape parameter $\frac{1}{2 ...
Katriel, Guy
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A New Evolution Model for Weighted Directed Networks
Xibei Gongye Daxue Xuebao, 2020 The most of the recent models of directed weighted network evolution capture the growth process based on two conventional assumptions: constant average degree assumption and slowly growing diameter assumption.
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Quantifying the Performance of Micro-Compartmentalized Directed Evolution Protocols
Life, 2020 High-throughput, in vitro approaches for the evolution of enzymes rely on a random micro-encapsulation to link phenotypes to genotypes, followed by screening or selection steps.
Adèle Dramé-Maigné +3 more
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Nitrene Transfer Catalyzed by a Non-Heme Iron Enzyme and Enhanced by Non-Native Small-Molecule Cofactors [PDF]
, 2019 Transition-metal catalysis is a powerful tool for the construction of chemical bonds. Here we show that a non-heme iron enzyme can catalyze olefin aziridination and nitrene C–H insertion, and that these activities can be improved by directed evolution ...
Arnold, Frances H. +3 more
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