Results 31 to 40 of about 286,859 (297)

Directing evolution in cells [PDF]

Nature Methods, 2020
A CRISPR–Cas9-based approach generates libraries of mutant proteins targeted to specific subcellular compartments for in situ directed evolution.
openaire   +2 more sources

amlalejini/directed-digital-evolution: 2022-03-31-initial-release

, 2022
Initial release for ...
Alex Lalejini
core   +1 more source

CRISPR enables directed evolution in plants

Genome Biology, 2019
A proof-of-concept study has demonstrated the application of CRISPR-Cas9 for directed evolution in rice, engineering crops for desired traits.
Yingxiao Zhang, Yiping Qi
doaj   +1 more source

Cis Elements: Added Boost to the Directed Evolution of Plant Genes

Journal of Pure and Applied Microbiology, 2022
To increase the expression of a native/foreign plant/bacterial gene, the complete network of cis-elements must be excavated to increase its biosynthetic yield, especially under industrial stress conditions.
Harisomayajula Valli, Devi Prasanna, Vikrant Singh Rajput, Wuriti Sridhar, N.N.V. Sakuntala, Padamata Harshavardhan, Ashish Runthala   +6 more
doaj   +1 more source

Directed evolution of a bright variant of mCherry: Suppression of non-radiative decay by fluorescence lifetime selections

, 2022
The approximately linear scaling of fluorescence quantum yield (QY) with fluorescence lifetime (τ) in fluorescent proteins (FPs) has inspired engineering of brighter fluorophores based on screening for increased lifetimes.
Srijit, Mukherjee, Premashis, Manna, Amy, Palmer, Felix, Vietmeyer, Pia, Friis, Ralph, Jimenez, Sheng-Ting, Hung   +6 more
core   +1 more source

Directed Evolution of Fungal Laccases [PDF]

Current Genomics, 2011
Fungal laccases are generalists biocatalysts with potential applications that range from bioremediation to novel green processes. Fuelled by molecular oxygen, these enzymes can act on dozens of molecules of different chemical nature, and with the help of redox mediators, their spectrum of oxidizable substrates is further pushed towards xenobiotic ...
Maté, Diana M., García-Ruiz, Eva, Camarero, Susana, Alcalde Galeote, Miguel   +3 more
openaire   +3 more sources

Disaccharide phosphorylases: Structure, catalytic mechanisms and directed evolution

Synthetic and Systems Biotechnology, 2021
Disaccharide phosphorylases (DSPs) are carbohydrate-active enzymes with outstanding potential for the biocatalytic conversion of common table sugar into products with attractive properties. They are modular enzymes that form active homo-oligomers. From a
Shangshang Sun, Chun You
doaj   +1 more source

Directed evolution in mammalian cells

, 2022
Directed evolution experiments are typically carried out using in vitro systems, bacteria, or yeast-even when the goal is to probe or modulate mammalian biology.
Hendel, Samuel J, Shoulders, Matthew D.
core   +1 more source

Deep sequencing revealed molecular signature of horizontal gene transfer of plant like transcripts in the mosquito Anopheles culicifacies: an evolutionary puzzle [version 1; referees: 2 approved]

F1000Research, 2015
In prokaryotes, horizontal gene transfer (HGT) has been regarded as an important evolutionary drive to acquire and retain beneficial genes for their survival in diverse ecologies.
Punita Sharma, Tanwee Das De, Swati Sharma, Ashwani Kumar Mishra, Tina Thomas, Sonia Verma, Vandana Kumari, Suman Lata, Namita Singh, Neena Valecha, Kailash Chand Pandey, Rajnikant Dixit   +11 more
doaj   +1 more source

PAIDDE: A Permutation-Archive Information Directed Differential Evolution Algorithm

IEEE Access, 2022
Evolutionary algorithms have shown great successes in various real-world applications ranging in molecule to astronomy. As a mainstream evolutionary algorithm, differential evolution (DE) possesses the characteristics of simple algorithmic structure ...
Xiaosi Li, Kaiyu Wang, Haichuan Yang, Sichen Tao, Shuai Feng, Shangce Gao   +5 more
doaj   +1 more source