Results 21 to 30 of about 11,830,288 (332)
Nature, 2021 Directed evolution is a powerful tool for improving existing properties and imparting completely new functionalities to proteins 1 – 4 . Nonetheless, its potential in even small proteins is inherently limited by the astronomical number of possible amino ...
Sagar Bhattacharya +10 more
semanticscholar +4 more sources
CRISPR enables directed evolution in plants [PDF]
Genome Biology, 2019 A proof-of-concept study has demonstrated the application of CRISPR-Cas9 for directed evolution in rice, engineering crops for desired traits.
Yingxiao Zhang, Yiping Qi
doaj +2 more sources
Directed evolution of hydrocarbon-producing enzymes [PDF]
Biotechnology for Biofuels and BioproductsEnzymes capable of catalysing the production of hydrocarbons hold promise for sustainable fuel synthesis. However, the native activities of these enzymes are often insufficient for their exploitation in industrial bioprocesses.
Jochem R. Nielsen +2 more
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CRISPR-Based Directed Evolution for Crop Improvement.
Trends in Biotechnology, 2020 Directed evolution involves generating diverse sequence variants of a gene of interest to produce a desirable trait under selective pressure. CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9) systems can ...
Haroon Butt +3 more
semanticscholar +2 more sources
CADEE: Computer-Aided Directed Evolution of Enzymes
IUCrJ, 2017 The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a ...
Beat Anton Amrein +5 more
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VEGAS as a Platform for Facile Directed Evolution in Mammalian Cells
Cell, 2019 SUMMARY Directed evolution, artificial selection toward designed objectives, is routinely used to develop new molecular tools and therapeutics. Successful directed molecular evolution campaigns repeatedly test diverse sequences with a designed selective ...
Bryan L. Roth +2 more
exaly +2 more sources
Optimisation strategies for directed evolution without sequencing. [PDF]
PLoS Computational BiologyDirected evolution can enable engineering of biological systems with minimal knowledge of their underlying sequence-to-function relationships. A typical directed evolution process consists of iterative rounds of mutagenesis and selection that are ...
Jessica James +3 more
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A chimeric viral platform for directed evolution in mammalian cells
Nature CommunicationsDirected evolution is a process of mutation and artificial selection to breed biomolecules with new or improved activity. Directed evolution platforms are primarily prokaryotic or yeast-based, and stable mammalian systems have been challenging to ...
Alexander J. Cole +6 more
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Directed evolution of CRISPR-Cas9 to increase its specificity
Nature Communications, 2018 Undesired off-target effects can hamper the use of CRISPR-Cas9 in therapeutic applications. Here the authors use a directed evolution approach to develop Sniper-Cas9 which combines high specificity with no loss of on-target activity.
Jungjoon K. Lee +10 more
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A risk based assessment approach for chemical mixtures from wastewater treatment plant effluents
Environment International, 2022 In this study, 56 effluent samples from 52 European wastewater treatment plants (WWTPs) were investigated for the occurrence of 499 emerging chemicals (ECs) and their associated potential risks to the environment.
Saskia Finckh +11 more
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