Results 11 to 20 of about 269,742 (193)

Directed evolution of hydrocarbon-producing enzymes [PDF]

Biotechnology for Biofuels and Bioproducts
Enzymes capable of catalysing the production of hydrocarbons hold promise for sustainable fuel synthesis. However, the native activities of these enzymes are often insufficient for their exploitation in industrial bioprocesses.
Jochem R. Nielsen, Joseph Kennerley, Wei E. Huang   +2 more
doaj   +2 more sources

Directed evolution of drug-like Aβ conformation-specific antibodies [PDF]

Frontiers in Immunology
Monoclonal antibodies that recognize conformational epitopes in protein aggregates are important for research, diagnostic, and therapeutic applications related to neurodegenerative disorders such as Alzheimer’s and Parkinson’s diseases. Unfortunately, it
Alec A. Desai, Alec A. Desai, Matthew D. Smith, Matthew D. Smith, Jennifer M. Zupancic, Jennifer M. Zupancic, Emily K. Makowski, Emily K. Makowski, Yulei Zhang, Yulei Zhang, Julia E. Gerson, Shannon J. Moore, Shannon J. Moore, Alexandra B. Sutter, Sean P. Ferris, Magdalena I. Ivanova, Magdalena I. Ivanova, Magdalena I. Ivanova, Geoffrey G. Murphy, Geoffrey G. Murphy, Geoffrey G. Murphy, Henry L. Paulson, Henry L. Paulson, Henry L. Paulson, Henry L. Paulson, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier   +30 more
doaj   +2 more sources

Active learning-assisted directed evolution [PDF]

Nature Communications
Directed evolution (DE) is a powerful tool to optimize protein fitness for a specific application. However, DE can be inefficient when mutations exhibit non-additive, or epistatic, behavior.
Jason Yang, Ravi G. Lal, James C. Bowden, Raul Astudillo, Mikhail A. Hameedi, Sukhvinder Kaur, Matthew Hill, Yisong Yue, Frances H. Arnold   +8 more
doaj   +2 more sources

A chimeric viral platform for directed evolution in mammalian cells [PDF]

Nature Communications
Directed evolution is a process of mutation and artificial selection to breed biomolecules with new or improved activity. Directed evolution platforms are primarily prokaryotic or yeast-based, and stable mammalian systems have been challenging to ...
Alexander J. Cole, Christopher E. Denes, Cesar L. Moreno, Lise Hunault, Thomas Dobson, Daniel Hesselson, G. Gregory Neely   +6 more
doaj   +2 more sources

CRISPR enables directed evolution in plants

Genome Biology, 2019
A proof-of-concept study has demonstrated the application of CRISPR-Cas9 for directed evolution in rice, engineering crops for desired traits.
Yingxiao Zhang, Yiping Qi
doaj   +1 more source

Directed evolution of improved zinc finger methyltransferases. [PDF]

PLoS ONE, 2014
The ability to target DNA methylation toward a single, user-designated CpG site in vivo may have wide applicability for basic biological and biomedical research.
Brian Chaikind, Marc Ostermeier
doaj   +1 more source

Cis Elements: Added Boost to the Directed Evolution of Plant Genes

Journal of Pure and Applied Microbiology, 2022
To increase the expression of a native/foreign plant/bacterial gene, the complete network of cis-elements must be excavated to increase its biosynthetic yield, especially under industrial stress conditions.
Harisomayajula Valli, Devi Prasanna, Vikrant Singh Rajput, Wuriti Sridhar, N.N.V. Sakuntala, Padamata Harshavardhan, Ashish Runthala   +6 more
doaj   +1 more source

CADEE: Computer-Aided Directed Evolution of Enzymes

IUCrJ, 2017
The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a ...
Beat Anton Amrein, Fabian Steffen-Munsberg, Ireneusz Szeler, Miha Purg, Yashraj Kulkarni, Shina Caroline Lynn Kamerlin   +5 more
doaj   +1 more source

Disaccharide phosphorylases: Structure, catalytic mechanisms and directed evolution

Synthetic and Systems Biotechnology, 2021
Disaccharide phosphorylases (DSPs) are carbohydrate-active enzymes with outstanding potential for the biocatalytic conversion of common table sugar into products with attractive properties. They are modular enzymes that form active homo-oligomers. From a
Shangshang Sun, Chun You
doaj   +1 more source

Deep sequencing revealed molecular signature of horizontal gene transfer of plant like transcripts in the mosquito Anopheles culicifacies: an evolutionary puzzle [version 1; referees: 2 approved]

F1000Research, 2015
In prokaryotes, horizontal gene transfer (HGT) has been regarded as an important evolutionary drive to acquire and retain beneficial genes for their survival in diverse ecologies.
Punita Sharma, Tanwee Das De, Swati Sharma, Ashwani Kumar Mishra, Tina Thomas, Sonia Verma, Vandana Kumari, Suman Lata, Namita Singh, Neena Valecha, Kailash Chand Pandey, Rajnikant Dixit   +11 more
doaj   +1 more source