Results 11 to 20 of about 11,830,288 (332)
Directed Evolution of a Mycobacteriophage [PDF]
Antibiotics, 2019 Bacteriophages represent an alternative strategy to combat pathogenic bacteria. Currently, Mycobacterium tuberculosis infections constitute a major public health problem due to extensive antibiotic resistance in some strains.
María Cebriá-Mendoza +2 more
doaj +5 more sources
Directed evolution: selection of the host organism
Computational and Structural Biotechnology Journal, 2012 Directed evolution has become a well-established tool for improving proteins and biological systems. A critical aspect of directed evolution is the selection of a suitable host organism for achieving functional expression of the target gene.
Azadeh Pourmir, Tyler W Johannes
doaj +6 more sources
Directed Evolution of Microbial Communities [PDF]
Annual Review of Biophysics, 2020 Directed evolution is a form of artificial selection that has been used for decades to find biomolecules and organisms with new or enhanced functional traits. Directed evolution can be conceptualized as a guided exploration of the genotype–phenotype map,
Álvaro Sánchez +5 more
semanticscholar +8 more sources
Directed evolution of an efficient and thermostable PET depolymerase [PDF]
Nature Catalysis, 2021 The recent discovery of IsPETase, a hydrolytic enzyme that can deconstruct poly(ethylene terephthalate) (PET), has sparked great interest in biocatalytic approaches to recycle plastics. Realization of commercial use will require the development of robust
Elizabeth S Bell +12 more
semanticscholar +3 more sources
Cell, 2021 Summary Replacing or editing disease-causing mutations holds great promise for treating many human diseases. Yet, delivering therapeutic genetic modifiers to specific cells in vivo has been challenging, particularly in large, anatomically distributed ...
Alexandra Stanton +2 more
exaly +2 more sources
Active learning-assisted directed evolution
Nature CommunicationsDirected evolution (DE) is a powerful tool to optimize protein fitness for a specific application. However, DE can be inefficient when mutations exhibit non-additive, or epistatic, behavior.
Jason Yang +8 more
doaj +2 more sources
Droplet Microfluidics and Directed Evolution of Enzymes: An Intertwined Journey
Angewandte Chemie - International Edition, 2021 Evolution is essential to the generation of complexity and ultimately life. It relies on the propagation of the properties, traits, and characteristics that allow an organism to survive in a challenging environment.
Petra S Dittrich +2 more
exaly +2 more sources
Directed Evolution in Mammalian Cells
Nature Methods, 2021 Directed evolution experiments are typically carried out using in vitro systems, bacteria, or yeast—even when the goal is to probe or modulate mammalian biology.
Samuel J. Hendel, M. Shoulders
semanticscholar +2 more sources
Sequence-free landscape inference for directed evolution. [PDF]
PLoS Computational BiologyDirected evolution is a method for engineering biological systems or components, such as proteins, wherein desired traits are optimised through iterative rounds of mutagenesis and selection of fit variants.
Sebastian Towers +3 more
doaj +2 more sources
A primer to directed evolution: current methodologies and future directions [PDF]
RSC Chemical Biology, 2023 Directed evolution is one of the most powerful tools for protein engineering and functions by harnessing natural evolution, but on a shorter timescale.
Lara Sellés Vidal +3 more
semanticscholar +2 more sources