Results 31 to 40 of about 17,272 (247)
Optimization of DNA shuffling for high fidelity recombination [PDF]
Nucleic Acids Research, 1997 A convenient 'DNA shuffling' protocol for random recombination of homologous genes in vitro with a very low rate of associated point mutagenesis (0.05%) is described. In addition, the mutagenesis rate can be controlled over a wide range by the inclusion of Mn2+or Mg2+during DNase I digestion, by choice of DNA polymerase used during gene reassembly as ...
Zhao, Huimin, Arnold, Frances H.
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Molecular Approaches to Improve the Insecticidal Activity of Bacillus thuringiensis Cry Toxins
Toxins, 2014 Bacillus thuringiensis (Bt) is a gram-positive spore-forming soil bacterium that is distributed worldwide. Originally recognized as a pathogen of the silkworm, several strains were found on epizootic events in insect pests.
Wagner A. Lucena +8 more
doaj +1 more source
Functional promoter analysis using an approach based on an in vitro evolution strategy
BioTechniques, 2005 In vitro evolution imitates the natural evolution of genes and has been very successfully applied to the modification of coding sequences, but it has not yet been applied to promoter sequences.
Tony Remans +3 more
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Engineering Microbiology, 2022 Polyethylene terephthalate (PET), one of the most widely used plastics in the world, causes serious environmental pollution. Recently, researchers have focused their efforts on enzymatic degradation of PET, which is an attractive way of degrading and ...
Xiaotong Wang +5 more
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Base eversion and shuffling by DNA methyltransferases
Chemistry & Biology, 1996 The structures of two DNA cytosine methyltransferases reveal two novel methods of gaining access to the substrate cytosine residue, both of which involve eversion of the cytosine in a process that may require DNA bending. In one instance there is also widespread base shuffling and distortion of the DNA.
Nelsons, Hillary C.M. +1 more
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Microarray-Based Method for Combinatorial Library Sequence Mapping and Characterization
BioTechniques, 2003 Here we describe a DNA-chip-based method for high-throughput sequence mapping. This involves competitive hybridization between short and differentially labeled fluorescent oligonucleotide probes and glass-supported PCR products.
V. Abécassis +6 more
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Pediatric Blood &Cancer, EarlyView.ABSTRACT Background An internal tandem duplication in the gene encoding Fms‐like tyrosine kinase 3 (FLT3‐ITD) is associated with high relapse risk and poor prognosis in acute myeloid leukemia (AML) and plays a crucial role in treatment decisions. Measurable residual disease (MRD) analysis of FLT3‐ITD during and after treatment has shown prognostic ...
Sofie Johansson Alm +11 more
wiley +1 more source
BioTechniques, 2016 Directed evolution has greatly facilitated protein engineering and provided new insights into protein structure—function relationships. DNA shuffling using restriction enzymes is a particularly simple and cost-effective means of recombinatorial evolution
Weiliang Huang +3 more
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A Bibliometric Analysis of Publications in Uremic Toxins From 1991 to 2024
Therapeutic Apheresis and Dialysis, EarlyView.ABSTRACT Background Uremic toxins are a growing area of research in nephrology, with significant implications in the progression and treatment of chronic kidney disease (CKD) and the management of end‐stage kidney disease (ESKD). This bibliometric analysis aims to evaluate the global research trends, key contributors, and the impact of publications in ...
Yuh‐Shan Ho +7 more
wiley +1 more source
Sequence permutations in the molecular evolution of DNA methyltransferases
BMC Evolutionary Biology, 2002 Background DNA methyltransferases (MTases), unlike MTases acting on other substrates, exhibit sequence permutation. Based on the sequential order of the cofactor-binding subdomain, the catalytic subdomain, and the target recognition domain (TRD), several
Bujnicki Janusz M
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