Enhanced Enzyme Performance by DNA Shuffling
CHIMIA, 1999 In the quest for enhanced enzyme performance in non-natural applications, directed evolution emulates natural processes for the generation of diversity and selection of desirable traits.
Andreas Crameri
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Shuffled: a software suite that assists the analysis of recombinant products resulting from DNA shuffling [PDF]
BioTechniques, 2004 DNA, Unshuffled In vitro recombination of gene variants for the purpose of generating libraries of novel chimeric genes is a powerful tool for the directed evolution of proteins.
Enrique Morett +1 more
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Development and functional analysis of novel genetic promoters using DNA shuffling, hybridization and a combination thereof. [PDF]
PLoS ONE, 2012 BACKGROUND: Development of novel synthetic promoters with enhanced regulatory activity is of great value for a diverse range of plant biotechnology applications.
Rajiv Ranjan +5 more
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Enhanced catalytic efficiency of CotA-laccase by DNA shuffling [PDF]
Bioengineered, 2019 Min Zhao
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Broadening the heterologous cross-neutralizing antibody inducing ability of porcine reproductive and respiratory syndrome virus by breeding the GP4 or M genes. [PDF]
PLoS ONE, 2013 Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important swine pathogens, which causes reproductive failure in sows and respiratory disease in piglets. A major hurdle to control PRRSV is the ineffectiveness of
Lei Zhou +8 more
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DNA shuffling as a tool for protein crystallization [PDF]
Proceedings of the National Academy of Sciences, 2005 The success of structural studies performed on an individual target in small scale or on many targets in the systemwide scale of structural genomics depends critically on three parameters: ( i ) obtaining an expression system capable of producing large quantities of the macromolecule(s) of ...
Robert J, Keenan +3 more
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Predicting crossover generation in DNA shuffling [PDF]
Proceedings of the National Academy of Sciences, 2001 We introduce a quantitative framework for assessing the generation of crossovers in DNA shuffling experiments. The approach uses free energy calculations and complete sequence information to model the annealing process. Statistics obtained for the annealing events then are combined with a reassembly algorithm to infer crossover ...
G L, Moore +3 more
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DNA profiling of saliva traces habitually deposited on various documents: a pilot study
Egyptian Journal of Forensic Sciences, 2020 Background A study was conducted to determine whether the habitualness among individuals to apply saliva to their fingertips (for moistening) while shuffling through pages of a document can provide evidence in cases pertaining to handling of documents in
Mukesh Kumar Thakar +4 more
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Computational and experimental analysis of DNA shuffling [PDF]
Proceedings of the National Academy of Sciences, 2003 We describe a computational model of DNA shuffling based on the thermodynamics and kinetics of this process. The model independently tracks a representative ensemble of DNA molecules and records their states at every stage of a shuffling reaction. These data can subsequently be analyzed to yield information on any relevant metric, including reassembly ...
Narendra, Maheshri, David V, Schaffer
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Revealing biases inherent in recombination protocols
BMC Biotechnology, 2007 Background The recombination of homologous genes is an effective protein engineering tool to evolve proteins. DNA shuffling by gene fragmentation and reassembly has dominated the literature since its first publication, but this fragmentation-based method
Tang Xiao-Song +6 more
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