Results 21 to 30 of about 17,272 (247)
Shuffling of DNA Cassettes in a Synthetic Integron [PDF]
, 2013 The complexity of even small gene networks makes them hardly amenable to rational design. Testing random combinations of genetic elements in a directed evolution procedure is thus of interest for many applications including metabolic engineering. Here we describe how the recombination machinery of class 1 integrons can be used to deliver and shuffle ...
Bikard, David, Mazel, Didier
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DNA Shuffling Method for Generating Estrogen Receptor α and β Chimeras in Yeast
BioTechniques, 2003 To facilitate our study of the molecular basis for the estrogen receptor (ER) subtype selectivity of novel ligands, we used DNA shuffling to construct chimeric ERs having ligand binding domains derived from both ERα and ERβ.
Jun Sun +3 more
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DNA shuffling brigthens prospects for GFP [PDF]
Nature Biotechnology, 1996 This article reviews a new technique emerging as an efficient way to direct protein evolution called `DNA shuffling`. This process uses the effective green fluorescent protein (GFP) as a control signal for certain proteins. 6 refs., 1 fig.
I, Matsumura, A D, Ellington
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Cassette hybridization for vector assembly application in antibody chain shuffling
BioTechniques, 2018 Gene assembly methods are an integral part of molecular cloning experiments. The majority of existing vector assembly methods stipulate a need for exonucleases, endonucleases and/or the use of single-stranded DNA as starting materials.
Jing Yi Lai +3 more
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DNA family shuffling of hyperthermostable β-glycosidases [PDF]
Biochemical Journal, 2002 The structural compatibility of two hyperthermostable family 1 glycoside hydrolases, Pyrococcus furiosus CelB and Sulfolobus solfataricus LacS, as well as their kinetic potential were studied by construction of a library of 2048 hybrid β-glycosidases using DNA family shuffling.
Kaper, T. +4 more
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Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes.
PLoS ONE, 2009 We have developed a protocol to assemble in one step and one tube at least nine separate DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained containing the desired construct.
Carola Engler +3 more
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Entropy, 2020 Aiming at the problems of small key space, low security of encryption structure, and easy to crack existing image encryption algorithms combining chaotic system and DNA sequence, this paper proposes an image encryption algorithm based on a hidden ...
Xin Jin +3 more
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Applications of mutagenesis methods on affinity maturation of antibodies in vitro
浙江大学学报. 农业与生命科学版, 2016 Antibodies selected from naive antibody libraries usually have low affinity at the micromolar level. Meanwhile, the affinities of natural antibodies obtained by repeated immunization were also observed to have a limit around 100 picomole because of the ...
LIU Yuan +7 more
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Scientific Reports, 2023 Scaffold-based protein libraries are designed to be both diverse and rich in functional/folded proteins. However, introducing an extended diversity while preserving stability of the initial scaffold remains a challenge.
M. Gomes +11 more
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Nature Communications, 2023 Biological modularity enhances evolutionary adaptability. This principle is vividly exemplified by bacterial viruses (phages), which display extensive genomic modularity. Phage genomes are composed of independent functional modules that evolve separately
Bogna J. Smug +4 more
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