Results 31 to 40 of about 169,578 (300)
STAR Protocols, 2022 Summary: Systematic evolution of ligands by exponential enrichment (SELEX) encompasses a wide variety of high-throughput screening techniques for producing nucleic acid binders to molecular targets through directed evolution.
Zachary William Cutts +7 more
doaj +1 more source
Exploring Protein Sequence Space Using Computationally Directed Recombination [PDF]
, 2006 Evolution has provided us with many protein sequences. However, these sequences represent a very small fraction of the possible sequences. In the laboratory, scientists have explored areas of sequence space not represented by natural proteins both to ...
Meyer, Michelle Margaret
core +1 more source
Molecules, 2023 The combination of wet-lab experimental data on multi-site combinatorial mutations and machine learning is an innovative method in protein engineering. In this study, we used an innovative sequence-activity relationship (innov’SAR) methodology based on ...
Gaolin Li +4 more
doaj +1 more source
Viruses, 2022 Coronaviruses (CoVs) constitute a large and diverse subfamily of positive-sense single-stranded RNA viruses. They are found in many mammals and birds and have great importance for the health of humans and farm animals. The current SARS-CoV-2 pandemic, as
Grigorios D. Amoutzias +5 more
doaj +1 more source
Big and Slow: Phylogenetic estimates of molecular evolution in baleen whales (Suborder Mysticeti).
, 2009 Published in a leading journal of molecular evolutionary, this article presents the largest databases available to date of mitochondrial and nuclear intron sequences to estimate the ‘neutral’ rate of molecular evolution in the largest species of animals ...
Jackson, JA +5 more
core +1 more source
Evolutionary Approaches to Study Cytochrome c Peroxidase
CHIMIA, 2001 Directed molecular evolution of enzymes and proteins has emerged as an extremely powerful method to create proteins with novel properties, both for practical applications as well as for mechanistic studies.
André Iffland +3 more
doaj +2 more sources
Recent progress in directed evolution of stereoselective monoamine oxidases
Bioresources and Bioprocessing, 2019 Monoamine oxidases (MAOs) use molecular dioxygen as oxidant to catalyze the oxidation of amines to imines. This type of enzyme can be employed for the synthesis of primary, secondary, and tertiary amines by an appropriate deracemization protocol ...
Jiaqi Duan +5 more
doaj +1 more source
Optimized expression and specific activity of IL-12 by directed molecular evolution [PDF]
Proceedings of the National Academy of Sciences, 2003 DNA delivery of IL-12 has shown promise in reducing the toxic side effects associated with administration of recombinant human (h)IL-12 protein while maintaining the ability to inhibit tumor growth and abolish tumor metastases in animal models. We have developed a more potent version of IL-12 by using DNA shuffling and screening to improve its ...
Steven R, Leong +5 more
openaire +2 more sources
Biomolecules, 2020 Two photoactivatable near infrared fluorescent proteins (NIR FPs) named “PAiRFP1” and “PAiRFP2” are formed by directed molecular evolution from Agp2, a bathy bacteriophytochrome of Agrobacterium tumefaciens C58.
Faez Iqbal Khan +4 more
doaj +1 more source
Molecular dynamics simulation of an entire cell
Frontiers in Chemistry, 2023 The ultimate microscope, directed at a cell, would reveal the dynamics of all the cell’s components with atomic resolution. In contrast to their real-world counterparts, computational microscopes are currently on the brink of meeting this challenge.
Jan A. Stevens +8 more
doaj +1 more source