Results 11 to 20 of about 169,578 (300)

Protein directed evolution

Revista de Química, 2008
Directed evolution allows us to explore protein functionalities not required in the natural environment. It mimics natural genetic processes and selective pressures. This approach is used when the molecular basis is not completely understood and rational
Roberto Laos
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Improved catalytic performance and molecular insight for lipoxygenase from Enterovibrio norvegicus via directed evolution

Frontiers in Bioengineering and Biotechnology, 2023
Lipoxygenase (LOX) holds significant promise for food and pharmaceutical industries. However, albeit its application has been hampered by low catalytic activity and suboptimal thermostability.
Bingjie Zhang, Huibing Chi, Juan Shen, Yang Tao, Zhaoxin Lu, Fengxia Lu, Ping Zhu   +6 more
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Directed molecular evolution of ADP-glucose pyrophosphorylase [PDF]

Proceedings of the National Academy of Sciences, 2002
ADP-glucose pyrophosphorylase catalyzes a rate-limiting reaction in prokaryotic glycogen and plant starch biosynthesis. Despite sharing similar molecular size and catalytic and allosteric regulatory properties, the prokaryotic and higher plant enzymes differ in higher-order protein structure.
Peter R, Salamone, I Halil, Kavakli, Casey J, Slattery, Thomas W, Okita   +3 more
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Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance

eLife, 2022
Alteration of antibiotic binding sites through modification of ribosomal RNA (rRNA) is a common form of resistance to ribosome-targeting antibiotics. The rRNA-modifying enzyme Cfr methylates an adenosine nucleotide within the peptidyl transferase center,
Kaitlyn Tsai, Vanja Stojković, Lianet Noda-Garcia, Iris D Young, Alexander G Myasnikov, Jordan Kleinman, Ali Palla, Stephen N Floor, Adam Frost, James S Fraser, Dan S Tawfik, Danica Galonić Fujimori   +11 more
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Enhanced thermal and alkaline stability of L-lysine decarboxylase CadA by combining directed evolution and computation-guided virtual screening

Bioresources and Bioprocessing, 2022
As an important monomer for bio-based nylons PA5X, cadaverine is mainly produced by enzymatic decarboxylation of L-lysine. A key issue with this process is the instability of L-lysine decarboxylase (CadA) during the reaction due to the dissociation of ...
Yang Xi, Lidan Ye, Hongwei Yu
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Modification of pancreatic lipase properties by directed molecular evolution [PDF]

Protein Engineering Design and Selection, 2010
Cystic fibrosis is associated with pancreatic insufficiency and acidic intraluminal conditions that limit the action of pancreatic enzyme replacement therapy, especially that of lipase. Directed evolution combined with rational design was used in the aim of improving the performances of the human pancreatic lipase at acidic pH.
Colin, D., Deprez-Beauclair, P., Silva, N., Infantes, L., Kerfelec, B.   +4 more
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Prediction of designer-recombinases for DNA editing with generative deep learning

Nature Communications, 2022
Design of recombinases with new target sites is usually achieved through cycles of directed molecular evolution. Here the authors report Recombinase Generator, RecGen, an algorithm for generation of designer-recombinases; they perform experimental ...
Lukas Theo Schmitt, Maciej Paszkowski-Rogacz, Florian Jug, Frank Buchholz   +3 more
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Deep sequencing revealed molecular signature of horizontal gene transfer of plant like transcripts in the mosquito Anopheles culicifacies: an evolutionary puzzle [version 1; referees: 2 approved]

F1000Research, 2015
In prokaryotes, horizontal gene transfer (HGT) has been regarded as an important evolutionary drive to acquire and retain beneficial genes for their survival in diverse ecologies.
Punita Sharma, Tanwee Das De, Swati Sharma, Ashwani Kumar Mishra, Tina Thomas, Sonia Verma, Vandana Kumari, Suman Lata, Namita Singh, Neena Valecha, Kailash Chand Pandey, Rajnikant Dixit   +11 more
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Known Evolutionary Paths Are Accessible to Engineered ß-Lactamases Having Altered Protein Motions at the Timescale of Catalytic Turnover

Frontiers in Molecular Biosciences, 2020
The evolution of new protein functions is dependent upon inherent biophysical features of proteins. Whereas, it has been shown that changes in protein dynamics can occur in the course of directed molecular evolution trajectories and contribute to new ...
Lorea Alejaldre, Lorea Alejaldre, Lorea Alejaldre, Claudèle Lemay-St-Denis, Claudèle Lemay-St-Denis, Claudèle Lemay-St-Denis, Carles Perez Lopez, Ferran Sancho Jodar, Victor Guallar, Victor Guallar, Joelle N. Pelletier, Joelle N. Pelletier, Joelle N. Pelletier, Joelle N. Pelletier   +13 more
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Improved Bioproduction of the Nylon 12 Monomer by Combining the Directed Evolution of P450 and Enhancing Heme Synthesis

Molecules, 2023
The nylon 12 (PA12) monomer ω-aminododecanoic acid (ω-AmDDA) could be synthesized from lauric acid (DDA) through multi-enzyme cascade transformation using engineered E.
Jiaming Yu, Jiawei Ge, Hongwei Yu, Lidan Ye   +3 more
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