Results 11 to 20 of about 493,855 (292)
Food Technology and Biotechnology, 2018 L-Cysteine sulfinate decarboxylase (CSD, EC 4.1.1.29), the rate-limiting enzyme in taurine synthesis pathway, catalyzes L-cysteine sulfinic acid to form hypotaurine.
Jie Deng +6 more
doaj +3 more sources
Rapid directed molecular evolution of fluorescent proteins in mammalian cells. [PDF]
Protein Sci, 2022 Abstract In vivo imaging of model organisms is heavily reliant on fluorescent proteins with high intracellular brightness. Here we describe a practical method for rapid optimization of fluorescent proteins via directed molecular evolution in cultured mammalian cells.
Babakhanova S +18 more
europepmc +6 more sources
Directed molecular evolution to design advanced red fluorescent proteins. [PDF]
Nat Methods, 2011 Fluorescent proteins have become indispensable imaging tools for biomedical research. Continuing progress in fluorescence imaging, however, requires probes with additional colors and properties optimized for emerging techniques. Here we summarize strategies for development of red-shifted fluorescent proteins.
Subach FV, Piatkevich KD, Verkhusha VV.
europepmc +4 more sources
Directed molecular evolution by somatic hypermutation [PDF]
Protein Engineering Design and Selection, 2004 After rearrangement of immunoglobulin gene segments, the immune system evolves the antibody repertoire by mutating the immunoglobulin variable region at a high rate. While this somatic hypermutation was thought to occur only at the variable region, recent studies suggest that hypermutation can occur at locations throughout the genome.
Clifford L, Wang +2 more
openaire +2 more sources
Directed molecular evolution of ADP-glucose pyrophosphorylase [PDF]
Proceedings of the National Academy of Sciences, 2002 ADP-glucose pyrophosphorylase catalyzes a rate-limiting reaction in prokaryotic glycogen and plant starch biosynthesis. Despite sharing similar molecular size and catalytic and allosteric regulatory properties, the prokaryotic and higher plant enzymes differ in higher-order protein structure.
Peter R, Salamone +3 more
openaire +2 more sources
Modification of pancreatic lipase properties by directed molecular evolution [PDF]
Protein Engineering Design and Selection, 2010 Cystic fibrosis is associated with pancreatic insufficiency and acidic intraluminal conditions that limit the action of pancreatic enzyme replacement therapy, especially that of lipase. Directed evolution combined with rational design was used in the aim of improving the performances of the human pancreatic lipase at acidic pH.
Colin, D. +4 more
openaire +3 more sources
The molecular evolution of feathers with direct evidence from fossils [PDF]
Proceedings of the National Academy of Sciences, 2019 Significance During the dinosaur–bird transition, feathers of bird ancestors must have been molecularly modified to become biomechanically suitable for flight. We report molecular moieties in fossil feathers that shed light on that transition. Pennaceous feathers attached to the right forelimb of the Jurassic dinosaur
Yanhong Pan +15 more
openaire +2 more sources
Molecules, 2023 The nylon 12 (PA12) monomer ω-aminododecanoic acid (ω-AmDDA) could be synthesized from lauric acid (DDA) through multi-enzyme cascade transformation using engineered E.
Jiaming Yu +3 more
doaj +1 more source
Protein engineering of Pseudomonas fluorescens peroxidase Dyp1B for oxidation of phenolic and polymeric lignin substrates [PDF]
, 2019 Directed evolution was applied to dye-decolourizing peroxidase Dyp1B from Pseudomonas fluorescens Pf-5, in order to enhance the activity for oxidation of phenolic and lignin substrates.
Ashley, Ben +6 more
core +2 more sources
Walks of molecular motors in two and three dimensions [PDF]
, 2002 Molecular motors interacting with cytoskeletal filaments undergo peculiar random walks consisting of alternating sequences of directed movements along the filaments and diffusive motion in the surrounding solution.
Adam G. +12 more
core +3 more sources