Results 21 to 30 of about 493,855 (292)

Directing the evolution of Rubisco and Rubisco activase: first impressions of a new tool for photosynthesis research [PDF]

, 2008
During the last decade the practice of laboratory-directed protein evolution has become firmly established as a versatile tool in biochemical research by enabling molecular evolution toward desirable phenotypes or detection of novel structure-function ...
Oliver Mueller-Cajar, Spencer M. Whitney, A Aharoni, I Andersson, TJ Andrews, TJ Andrews, FH Arnold, FH Arnold, FH Arnold, FH Arnold, H Ashida, H Ashida, G Bainbridge, S Bershtein, LA Castle, Z Chen, LP Cloney, SJ Crafts-Brandner, A Crameri, AM Dean, DA Drummond, YC Du, D Emlyn-Jones, J Gewolb, DN Greene, AD Griffiths, LM Guzman, TE Hanson, GS Hudson, TW Johannes, K Kim, I Kurek, HN Lin, SP Long, M Ludwig, CC Mann, I Matsumura, J Maynard Smith, OJ Miller, MK Morell, O Mueller-Cajar, C Neylon, WL Ogren, GC Paoli, MR Parikh, MR Parikh, MAJ Parry, MAJ Parry, SG Peisajovich, J Pierce, FJ Poelwijk, AR Portis, C Roodveldt, ME Salvucci, ME Salvucci, ME Salvucci, S Saschenbrecker, C Schmidt-Dannert, TD Sharkey, SA Smith, CR Somerville, CR Somerville, RJ Spreitzer, RJ Spreitzer, RJ Spreitzer, RJ Spreitzer, WPC Stemmer, GB Tcherkez, G Thow, CA Voigt, GS Waldo, GMF Watson, SM Whitney, SM Whitney, A Wingler, KJ Woycechowsky, Y Yoshikuni, L Yuan, XG Zhu   +78 more
core   +1 more source

Directed Evolution of Protein-Based Neurotransmitter Sensors for MRI [PDF]

, 2013
The production of contrast agents sensitive to neuronal signaling events is a rate-limiting step in the development of molecular-level functional magnetic resonance imaging (molecular fMRI) approaches for studying the brain.
A Jasanoff, AA Gilad, B Iordanova, DFV Lewis, JD Bloom, MA Bernstein, MG Shapiro, MG Shapiro, MJ Dougherty, MT McMahon, R Fasan, T Omura   +11 more
core   +1 more source

How special is the biochemical function of native proteins? [version 1; referees: 2 approved]

F1000Research, 2016
Native proteins perform an amazing variety of biochemical functions, including enzymatic catalysis, and can engage in protein-protein and protein-DNA interactions that are essential for life.
Jeffrey Skolnick, Mu Gao, Hongyi Zhou
doaj   +1 more source

Molecular Modeling in Enzyme Design, Toward In Silico Guided Directed Evolution [PDF]

, 2017
Directed evolution (DE) creates diversity in subsequent rounds of mutagenesis in the quest of increased protein stability, substrate binding, and catalysis.
Acebes, Sandra, Guallar, Victor, Lucas, Fátima, Monza, Emanuele   +3 more
core   +2 more sources

Exploiting Models of Molecular Evolution to Efficiently Direct Protein Engineering [PDF]

Journal of Molecular Evolution, 2010
Directed evolution and protein engineering approaches used to generate novel or enhanced biomolecular function often use the evolutionary sequence diversity of protein homologs to rationally guide library design. To fully capture this sequence diversity, however, libraries containing millions of variants are often necessary. Screening libraries of this
Megan F, Cole, Eric A, Gaucher
openaire   +2 more sources

Evolution of a Landscape Phage Library in a Mouse Xenograft Model of Human Breast Cancer

Viruses, 2019
Peptide-displayed phage libraries are billion-clone collections of diverse chimeric bacteriophage particles, decorated by genetically fused peptides built from a random combination of natural amino acids.
James W. Gillespie, Liping Yang, Laura Maria De Plano, Murray A. Stackhouse, Valery A. Petrenko   +4 more
doaj   +1 more source

On the entropy of protein families [PDF]

, 2015
Proteins are essential components of living systems, capable of performing a huge variety of tasks at the molecular level, such as recognition, signalling, copy, transport, ...
Barton, John, Chakraborty, Arup, Cocco, Simona, Jacquin, Hugo, Monasson, Rémi   +4 more
core   +4 more sources

Directed enzyme evolution: climbing fitness peaks one amino acid at a time [PDF]

, 2009
Directed evolution can generate a remarkable range of new enzyme properties. Alternate substrate specificities and reaction selectivities are readily accessible in enzymes from families that are naturally functionally diverse.
Arnold, Frances H., Tracewell, Cara A.
core   +2 more sources

CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors

Genome Biology, 2019
Increasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based directed evolution platform in plants to evolve the rice (Oryza sativa) SF3B1 spliceosomal ...
Haroon Butt, Ayman Eid, Afaque A. Momin, Jeremie Bazin, Martin Crespi, Stefan T. Arold, Magdy M. Mahfouz   +6 more
doaj   +1 more source

Target-switch SELEX: Screening with alternating targets to generate aptamers to conserved terminal dipeptides

STAR Protocols, 2022
Summary: Systematic evolution of ligands by exponential enrichment (SELEX) encompasses a wide variety of high-throughput screening techniques for producing nucleic acid binders to molecular targets through directed evolution.
Zachary William Cutts, Jessica M. Hong, Shirley Shao, Alexander Tran, Michelle Dimon, Marc Berndl, Diana Wu, Annalisa Pawlosky   +7 more
doaj   +1 more source