Results 21 to 30 of about 169,578 (300)
The molecular evolution of feathers with direct evidence from fossils [PDF]
Proceedings of the National Academy of Sciences, 2019 Significance During the dinosaur–bird transition, feathers of bird ancestors must have been molecularly modified to become biomechanically suitable for flight. We report molecular moieties in fossil feathers that shed light on that transition. Pennaceous feathers attached to the right forelimb of the Jurassic dinosaur
Yanhong Pan +15 more
openaire +2 more sources
Computationally Optimizing the Directed Evolution of Proteins [PDF]
, 2003 Directed evolution has proven a successful strategy for protein engineering. To accelerate the discovery process, we have developed several computational methods to optimize the mutant libraries by targeting specific residues for mutagenesis, and ...
Voigt, Christopher Ashby
core +1 more source
Statistical Models of the Protein Fitness Landscape: Applications to Protein Evolution and Engineering [PDF]
, 2012 Understanding the protein fitness landscape is important for describing how natural proteins evolve and for engineering new proteins with useful properties.
Romero, Philip Anthony
core +1 more source
How special is the biochemical function of native proteins? [version 1; referees: 2 approved]
F1000Research, 2016 Native proteins perform an amazing variety of biochemical functions, including enzymatic catalysis, and can engage in protein-protein and protein-DNA interactions that are essential for life.
Jeffrey Skolnick, Mu Gao, Hongyi Zhou
doaj +1 more source
Molecular analyses of CRISPR-directed gene editing on the NRF2 gene
, 2021 Kmiec, Eric B.Lung cancer remains the leading cause of cancer-related death worldwide. As a result, the prognosis of patients diagnosed with Non-small cell lung carcinoma (NSCLC), particularly, is dismal indicating the need for continued improvement in ...
Banas, Kelly Helena
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Evolution of a Landscape Phage Library in a Mouse Xenograft Model of Human Breast Cancer
Viruses, 2019 Peptide-displayed phage libraries are billion-clone collections of diverse chimeric bacteriophage particles, decorated by genetically fused peptides built from a random combination of natural amino acids.
James W. Gillespie +4 more
doaj +1 more source
Exploiting Models of Molecular Evolution to Efficiently Direct Protein Engineering [PDF]
Journal of Molecular Evolution, 2010 Directed evolution and protein engineering approaches used to generate novel or enhanced biomolecular function often use the evolutionary sequence diversity of protein homologs to rationally guide library design. To fully capture this sequence diversity, however, libraries containing millions of variants are often necessary. Screening libraries of this
Megan F, Cole, Eric A, Gaucher
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A primer to directed evolution: current methodologies and future directions [PDF]
, 2023 Directed evolution is one of the most powerful tools for protein engineering and functions by harnessing natural evolution, but on a shorter timescale.
Isalan, Mark +5 more
core +1 more source
CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors
Genome Biology, 2019 Increasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based directed evolution platform in plants to evolve the rice (Oryza sativa) SF3B1 spliceosomal ...
Haroon Butt +6 more
doaj +1 more source
, 2022 Pyrrolysyl-tRNA synthetase (PylRS) is frequently used for site-specific incorporation of noncanonical amino acids (ncAAs) into proteins. Recently, the active site of Methanomethylophilus alvus PylRS (MaPylRS) has been rationally engineered to expand its ...
Tharp, Jeffery M. +2 more
core +1 more source