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Directed Evolution of Aldolases
2004Publisher Summary This chapter describes the directed evolution of aldolases. The detailed protocols for generating aldolases with new catalytic properties are presented. The gene encoding aldolase can be amplified using the standard polymerase chain reaction (PCR) from a genomic DNA preparation using the Qiagen kit and primers flanking the gene with
Dirk, Franke +2 more
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Rapid in silico directed evolution by a protein language model with EVOLVEpro.
ScienceDirected protein evolution is central to biomedical applications but faces challenges like experimental complexity, inefficient multi-property optimization, and local maxima traps.
Kai-Yi Jiang +11 more
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The Evolution of Advance Directives
JAMA, 2011THERE ARE 2 WIDELY KNOWN PROBLEMS WITH END-OFlife care in the United States. First, care often does not reflect patients’ values and preferences. Second, end-of-life care constitutes a disproportionate amount of health care expenditures. There is a need to improve the value of health care spending because of changing economic and political climates and
Douglas B, White, Robert M, Arnold
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Origins of life and evolution of the biosphere, 1999
We propose the existence of a relationship of stereochemical complementarity between gene sequences that code for interacting components: nucleic acid-nucleic acid, protein-protein and protein-nucleic acid. Such a relationship would impose evolutionary constraints on the DNA sequences themselves, thus retaining these sequences and governing the ...
L F, Harris +2 more
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We propose the existence of a relationship of stereochemical complementarity between gene sequences that code for interacting components: nucleic acid-nucleic acid, protein-protein and protein-nucleic acid. Such a relationship would impose evolutionary constraints on the DNA sequences themselves, thus retaining these sequences and governing the ...
L F, Harris +2 more
openaire +2 more sources
Emerging Topics in Life Sciences, 2020
There are near-to-infinite combinations of possibilities for evolution to happen within nature, making it yet impossible to predict how it occurs. However, science is now able to understand the mechanisms underpinning the evolution of biological systems and can use this knowledge to experimentally mimic nature.
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There are near-to-infinite combinations of possibilities for evolution to happen within nature, making it yet impossible to predict how it occurs. However, science is now able to understand the mechanisms underpinning the evolution of biological systems and can use this knowledge to experimentally mimic nature.
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Directed evolution of biocatalysts
Current Opinion in Chemical Biology, 1999Directed evolution is being used increasingly in academic and industrial laboratories to modify and improve important biocatalysts. Significant advances during this period of review include compartmentalization of genes and the in vitro translation apparatus in emulsions, as well as several impressive demonstrations of catalyst improvement.
Arnold, Frances H., Volkov, Alexander A.
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Directed evolution: selecting today's biocatalysts
Biomolecular Engineering, 2005Directed evolution has become a full-grown tool in molecular biology nowadays. The methods that are involved in creating a mutant library are extensive and can be divided into several categories according to their basic ideas. Furthermore, both screening and selection can be used to target the enzyme towards the desired direction.
Otten, Linda, Quax, Wim
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Angewandte Chemie International Edition, 1998
On the way to a combinatorial biotechnology? The directed evolution of enzymes promises a rapid access to effective biocatalysts. New molecular biology techniques for random mutagenesis in combination with high-throughput screening might revolutionize the creation of enzymes with new and improved properties.
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On the way to a combinatorial biotechnology? The directed evolution of enzymes promises a rapid access to effective biocatalysts. New molecular biology techniques for random mutagenesis in combination with high-throughput screening might revolutionize the creation of enzymes with new and improved properties.
openaire +2 more sources
Directed Evolution of Adenoviruses
2016The ability to evolve viruses in cell culture in the face of selective pressure is an invaluable method to elucidate the molecular mechanisms of synthetic or natural antivirals, expand tropism, or alter virulence. Recently, mutations to the human adenovirus polymerase that reduce replicative fidelity were described, and we have incorporated one of ...
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Accounts of Chemical Research, 2019
Transition metal catalysts mediate a wide variety of chemo-, stereo-, and regioselective transformations, and therefore play a pivotal role in modern synthetic organic chemistry.
M. Reetz
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Transition metal catalysts mediate a wide variety of chemo-, stereo-, and regioselective transformations, and therefore play a pivotal role in modern synthetic organic chemistry.
M. Reetz
semanticscholar +1 more source