Results 61 to 70 of about 3,134,594 (306)

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Partial Dissociation and Hydration Quantitatively Explain the Properties of Aqueous Electrolyte Solutions and hence Empirical Activity Concepts are Unnecessary [PDF]

, 2011
The author shows that Arrhenius's theory of partial electrolytic dissociation was immaturely displaced about eight decades ago by the empirical concept of ionic activities and the assumption of complete dissociation of electrolytes at all ...
Raji Heyrovska
core  

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Click chemistry with DNA [PDF]

, 2010
The advent of click chemistry has led to an influx of new ideas in the nucleic acids field. The copper catalysed alkyne–azide cycloaddition (CuAAC) reaction is the method of choice for DNA click chemistry due to its remarkable efficiency.
El-Sagheer, Afaf H., Brown, Tom
core   +1 more source

Model for acid-base chemistry in nanoparticle growth (MABNAG) [PDF]

Atmospheric Chemistry and Physics, 2013
Climatic effects of newly-formed atmospheric secondary aerosol particles are to a large extent determined by their condensational growth rates. However, all the vapours condensing on atmospheric nanoparticles and growing them to climatically relevant ...
T. Yli-Juuti, K. Barsanti, L. Hildebrandt Ruiz, A.-J. Kieloaho, U. Makkonen, T. Petäjä, T. Ruuskanen, M. Kulmala, I. Riipinen   +8 more
doaj   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

Bond Dissociation Energies of Carbonyl Gold Complexes: a New Descriptor of Ligand Effects in Gold(I) Complexes?

, 2018
International audienceLigand electronic effects in gold(I) chemistry have been evaluated by means of the experimental determination of M-CO bond dissociation energies for 16 [L-Au-CO] + complexes, bearing L ligands widely used in gold catalysis.
Cole, Richard, Denis Lesage, Richard B. Cole, Héloïse Dossmann, Gimbert, Yves, Yves Gimbert, Gatineau, David, Chan, Chen, Chen H. Chan, Hervé Clavier, Dossmann, Heloise, Anne Milet, Cole, Richard, B, Memboeuf, Antony, Lesage, Denis, Clavier, Hervé, Antony Memboeuf, Chan, Chen-Hui, Milet, Anne, David Gatineau   +19 more
core   +1 more source

Probing the structure of nanodiscs using surface-induced dissociation mass spectrometry

, 2020
In the study of membrane proteins and antimicrobial peptides, nanodiscs have emerged as a valuable membrane mimetic to solubilze these molecules in a lipid bilayer.
VanAernum, Zachary L, Marty, Michael T, Kostelic, Marius M, Harvey, Sophie R, Wysocki, Vicki H, Zachary L. VanAernum, Marius M. Kostelic, Sophie R. Harvey, Vicki H. Wysocki, Michael T. Marty   +9 more
core   +1 more source

iBonD: bond energy database embracing the era of artificial intelligence [PDF]

Digital Discovery
The lack of a systematically curated and readily accessible bond energy database has long limited the effective use of acidity constant (pKa) and bond dissociation energy (BDE) data in synthetic chemistry.
Shen Wang, Qi Yang, Sanzhong Luo, Jin-Pei Cheng   +3 more
doaj   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source