Results 221 to 230 of about 271,886 (306)

Protocol for simultaneous profiling of N- and O-glycans on glycoproteins using a one-pot format. [PDF]

STAR Protoc
Knizner KT, Gass DT, Matsumoto Y, Ortega-Rodriguez U, Bettinger JQ, Ju T.   +5 more
europepmc   +1 more source

Oxygen‐independent expression of HIF‐1α during the cell cycle in hepatocellular carcinoma cells controls essential metabolic pathways under normoxia

The FEBS Journal, EarlyView.
In Huh7 cells, HIF‐1α is essential as it maintains the expression of proteins involved in glycolysis and steroid/cholesterol biosynthesis both under normoxia and hypoxia. On the other hand, in HeLa cells, these pathways are induced by HIF‐1α only under hypoxia.
Ioanna‐Maria Gkotinakou, Christina Arseni, Kreon Koukoulas, Martina Samiotaki, George Panayotou, George Simos, Ilias Mylonis   +6 more
wiley   +1 more source

Correction: Biochemical and structural characterization of the human gut microbiome metallopeptidase IgAse provides insight into its unique specificity for the Fab' region of IgA1 and IgA2. [PDF]

PLoS Pathog
Ramírez-Larrota JS, Juyoux P, Guerra P, Eckhard U, Gomis-Rüth FX.   +4 more
europepmc   +1 more source

Plant organelle C‐to‐U RNA editing factors can operate successfully in yeast (Saccharomyces cerevisiae) as an easily amenable eukaryotic system for their functional analysis

The FEBS Journal, EarlyView.
Plant‐type pentatricopeptide repeat proteins capable of C‐to‐U RNA editing perform faithfully when expressed in a new heterologous system, the yeast Saccharomyces cerevisiae. They were tested with constitutive and inducible expression and with a set of different solubility tags. PPR56, PPR65, and PPR78 from P.
Shyam Ramanathan, Annika Hanraths, Jingchan Xie, Mareike Schallenberg‐Rüdinger, Volker Knoop   +4 more
wiley   +1 more source

Desensitization With Imlifidase: Overcoming Immunological Barriers in Kidney Transplantation. [PDF]

Kidney Med
Hernández-Velasco PJ, Gutiérrez Martínez E, Polanco Fernández N, González Monte ME, González-García C, Mancebo Sierra E, Andrés Belmonte A.   +6 more
europepmc   +1 more source

The ribosome‐associated complex regulates cytosolic translation upon mitoprotein‐induced stress

The FEBS Journal, EarlyView.
Defects in mitochondrial protein import trigger a stress response. The contribution of translation regulation to such a response is only partially understood. We demonstrate that the deletion of mitochondrial surface proteins (TOM70/TOM71) leads to defects in mitochondrial biogenesis and slow growth.
Jiaxin Qian, Annika Nutz, Katja Hansen, Lea Bertgen, Mirita Franz‐Wachtel, Boris Macek, Johannes M. Herrmann, Doron Rapaport   +7 more
wiley   +1 more source

Absence of Red Blood Cell Alloimmunization in Transfused Patients Receiving Daratumumab: Experience from a Single Center. [PDF]

J Clin Med
Eritzpokhoff L, Talegón De La Fuente E, Carril Barcia A, Asensi Cantó P, Gómez Segui I, Arnao Herraiz M, De La Rubia Comos J, Solves Alcaina P.   +7 more
europepmc   +1 more source

Characterisation of a cold‐adapted, thermostable glucokinase from psychrophilic Pseudoalteromonas sp. AS‐131 reveals how the enzyme achieves high thermal stability without loss of cold adaptation

The FEBS Journal, EarlyView.
We investigated glucokinase from the psychrophilic Pseudoalteromonas sp. AS‐131 (PsGK), which was isolated from the Antarctic Ocean. Biochemical studies revealed that PsGK is a cold‐adapted enzyme with high thermal stability, in contrast to general cold‐adapted enzymes, which have low thermal stability.
Akane Yato, Yuki Kato, Fuyuko Hayashi, Rio Asaka, Atsuko Ogawa, Tokuro Oda, Sayaka Tsuji, Masashi Unno, Nobuaki Soh, Keiichi Watanabe, Masaki Horitani   +10 more
wiley   +1 more source

Dissociation of IgM antibodies from red blood cells using ethylenediamine tetraacetate/glycine acid or chloroquine diphosphate. [PDF]

Transfusion
Abe Y, Matsuura H, Yamada A, Nakagawa R, Kojima H, Ishihara Y, Doi H, Fujii S, Miura Y.   +8 more
europepmc   +1 more source

Enzymatic synthesis of bioactive quinolones and (thio)coumarins by fungal type III polyketide synthases

The FEBS Journal, EarlyView.
Quinolones are valuable scaffolds for drug discovery but are rare in nature. Here, we show that two fungal enzymes, AthePKS and FerePKS, can generate 2‐quinolones and two additional heteroaromatic scaffolds. Using AthePKS, we designed an artificial enzymatic cascade towards an antimicrobial quinolone from a simple precursor and implemented it in E ...
Nika Sokolova, Angelina Osipyan, Lili Zhang, Matthew R. Groves, Sandy Schmidt, Kristina Haslinger   +5 more
wiley   +1 more source