Results 261 to 270 of about 270,943 (291)

Caffeine and dithiothreitol delay ovine oocyte ageing

Reproduction, Fertility and Development, 2010
The intracellular glutathione levels and developmental competence of aged oocytes after parthenogenetic activation, somatic cell nuclear transfer and intracytoplasmic sperm injection in the presence or absence of caffeine or dithiothreitol (DTT) were examined.
Xiao-Fang, Ye, Shi-Bin, Chen, Li-Qin, Wang, Yun-Cheng, Zhao, Xue-Feng, Lv, Ming-Jun, Liu, Jun-Cheng, Huang   +6 more
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Renal cortical mercury distribution following dithiothreitol administration

Journal of Toxicology and Environmental Health, 1985
Previous studies from this laboratory have indicated that dithiothreitol (DTT) administration (31 mg DTT/kg, i.p.) 1 h after mercuric chloride injection (3 mg/kg, i.v.) results in partial reversal of Hg-induced renal toxicity without altering total renal mercury deposition.
D R, Klonne, D R, Johnson
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Vitamin B12b enhances the cytotoxicity of dithiothreitol

Free Radical Biology and Medicine, 2008
It has been found previously that vitamin B12b amplifies significantly the cytotoxic effects of ascorbic acid by catalyzing the formation of reactive oxygen species, and the antioxidant dithiothreitol (DTT), in contrast to catalase, does not prevent the cytotoxicity.
Marina E, Solovieva, Valery V, Solovyev, Andrei A, Kudryavtsev, Yuliya A, Trizna, Vladimir S, Akatov   +4 more
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Rethinking Dithiothreitol-Based Particulate Matter Oxidative Potential: Measuring Dithiothreitol Consumption versus Reactive Oxygen Species Generation

Environmental Science & Technology, 2017
We measured the rate of generation of reactive oxygen species (ROS) [hydroxyl radicals (•OH) and hydrogen peroxide (H2O2)] catalyzed by ambient particulate matter (PM) in the dithiothreitol (DTT) assay. To understand the mechanism of ROS generation, we tested several redox-active substances, such as 9,10-phenanthrenequinone (PQ), 5-hydroxy-1,4 ...
Qianshan Xiong, Haoran Yu, Runran Wang, Jinlai Wei, Vishal Verma   +4 more
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Toxicity of the Sulfhydryl-Containing Radioprotector Dithiothreitol

Radiation Research, 1987
The toxicity of the sulfhydryl-containing radioprotective agent dithiothreitol (DTT) has been studied using Chinese hamster V79 cells growing in monolayer in minimal essential medium containing 10% fetal calf serum. DTT at low concentrations (between 0.4 and 1.0 mM) caused cell killing, but higher concentrations (above 2 mM) or lower concentrations (0 ...
K D, Held, D C, Melder
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Stability constant for the zinc-dithiothreitol complex

Analytical Biochemistry, 1972
Rat liver histidase is competitively inhibited by a variety of compounds that are good complexing agents for Zn+2. Dithiothreitol is a strong competitive inhibitor, but 20 μM Zn+2 completely reverses the inhibitory action of 1.7 mM dithiothreitol. Effectiveness of inhibitors is directly related to the stability constants (Kst) for the corresponding ...
N W, Cornell, K E, Crivaro
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Inactivation of the pseudorabies virus by dithiothreitol

Virology, 1971
Abstract The virucidal nature of reduced dithiothreitol (DTT) for the pseudorabies (PR) virus (PRV) is presented. The rate of decay of PRV in DTT increased exponentially as the pH rose from 6.5 to 8. Effective virucidal concentrations of DTT decreased in concentration as the pH was elevated.
J H, Gainer, J, Long, P, Hill, W I, Capps   +3 more
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Inactivation of purified phenylalanine hydroxylase by dithiothreitol

Biochemical and Biophysical Research Communications, 1992
Purified rat liver phenylalanine hydroxylase is inactivated in vitro by ascorbate and thiol compounds, dithiothreitol being the most effective inhibitor, with a second order rate constant for the inactivation of 0.066 +/- 0.002 mM-1.min-1 at 20 degrees C and pH 7.2.
A, Martínez, S, Olafsdottir, J, Haavik, T, Flatmark   +3 more
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1,4-Dithiothreitol

2001
[27565-41-9] C4H10O2S2 (MW 154.28) InChI = 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1 InChIKey = VHJLVAABSRFDPM-QWWZWVQMSA-N (reducing agent for disulfides;1, 2 protection of thiols and proteins containing free SH groups from air oxidation3) Alternate Names: DTT; threo-2,3-dihydroxybutane-1,4-dithiol ...
openaire   +1 more source

Reduction of Chymotrypsin A_α by Dithiothreitol

Canadian Journal of Biochemistry, 1971
Treatment of chymotrypsin Aα with dithiothreitol (10 mM) at pH 9.2 in the presence of 8 M urea results in the complete reduction of the disulfide linkages of the protein. Reduction of a single disulfide bond of the enzyme can be achieved by treatment with 10 mM dithiothreitol at pH 9.2 in the presence of 100 mM hydrocinnamate.
M, Stanton, T, Viswanatha
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