Results 11 to 20 of about 60,289 (232)
DNA replication: archaeal oriGINS [PDF]
BMC Biology, 2011 GINS is an essential eukaryotic DNA replication factor that is found in a simplified form in Archaea. A new study in this issue of BMC Biology reveals the first structure of the archaeal GINS complex.
Bell Stephen D
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Affinity Purification of an Archaeal DNA Replication Protein Network [PDF]
mBio, 2010 Nineteen Thermococcus kodakarensis strains have been constructed, each of which synthesizes a different His6-tagged protein known or predicted to be a component of the archaeal DNA replication machinery.
Zhuo Li +4 more
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Specific DNA binding of archaeal histones HMfA and HMfB
Frontiers in Microbiology, 2023 In archaea, histones play a role in genome compaction and are involved in transcription regulation. Whereas archaeal histones bind DNA without sequence specificity, they bind preferentially to DNA containing repeats of alternating A/T and G/C motifs ...
Amanda M. Erkelens +5 more
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Specificity and Function of Archaeal DNA Replication Initiator Proteins [PDF]
Cell Reports, 2013 Chromosomes with multiple DNA replication origins are a hallmark of Eukaryotes and some Archaea. All eukaryal nuclear replication origins are defined by the origin recognition complex (ORC) that recruits the replicative helicase MCM(2-7) via Cdc6 and ...
Rachel Y. Samson +10 more
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Eukaryogenesis: Did an Oxidative Crucible Result in Misleading Bioinformatic Analyses? [PDF]
BioessaysRecently, Nature published a timed gene duplication analysis favoring gradual models for eukaryogenesis. However, high initial mutation rates due to endogenous ROS formation by the OXPHOS system of the future mitochondrion and ensuing positive selection for countermeasures might strongly inflate branch lengths in such analyses. Ruling out symbiogenesis
Speijer D.
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Annual Review of Microbiology, 2020 It is now well recognized that the information processing machineries of archaea are far more closely related to those of eukaryotes than to those of their prokaryotic cousins, the bacteria. Extensive studies have been performed on the structure and function of the archaeal DNA replication origins, the proteins that define them, and the macromolecular
Mark D, Greci, Stephen D, Bell
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The archaeal Ced system imports DNA [PDF]
Proceedings of the National Academy of Sciences, 2016 Significance Among bacteria, transfer of DNA has been studied in great detail. Several bacterial DNA transfer systems have been described on a molecular level including competence and conjugation systems. In Archaea, DNA exchange has been observed for a number of organisms and its importance for horizontal gene transfer and DNA ...
Wolferen, Marleen van +3 more
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Using archaeal histones for precise DNA fragmentation [PDF]
Protein Engineering Design and Selection, 2007 The fragmentation of DNA is a useful procedure for many molecular biology procedures. However, most methods used to fragment DNA are poorly controllable, and cannot be used to create small fragments. We describe a method to generate random DNA fragments of a predictable size to be cloned in expression vectors for the construction of display libraries ...
AZZONI E +4 more
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Nature Communications, 2022 DNA primases initiate a short primer before handing off to DNA polymerases to continue replication. Here the authors reveal a unique ability of archaeal primases to first synthesize RNA before stochastically incorporating a deoxyribonucleotide and ...
Mark D. Greci +2 more
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Archaea: A Gold Mine for Topoisomerase Diversity
Frontiers in Microbiology, 2021 The control of DNA topology is a prerequisite for all the DNA transactions such as DNA replication, repair, recombination, and transcription. This global control is carried out by essential enzymes, named DNA-topoisomerases, that are mandatory for the ...
Florence Garnier +6 more
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