Results 271 to 280 of about 854,281 (317)
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Bacterial Dna in Mixed Cholesterol Gallstones
American Journal of Gastroenterology, 1999Numerous investigators have proposed a role for bacteria in biliary lithogenesis. We hypothesized that bacterial DNA is present in gallstones, and that categorical differences exist between gallstone type and the frequency of bacterial sequences.Polymerase chain reaction (PCR) was used to amplify bacterial 16S rRNA and uidA (encoding Escherichia coli ...
Haigh, WG, Tarr, PI, Lee, DK, Lee, SP
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Comparing Bacterial DNA Microarray Fingerprints
Statistical Applications in Genetics and Molecular Biology, 2005Epidemiologic and forensic investigations often require assays to detect subtle genetic differences between closely related microorganisms. Typically, gel electrophoresis is used to compare randomly amplified DNA fragments between microbial samples, where the patterns of DNA fragment sizes are viewed as genotype fingerprints.
Willse, Alan +5 more
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Bacterial DNA in the diagnosis of spontaneous bacterial peritonitis.
Alimentary pharmacology & therapeutics, 2011Despite inoculation into blood culture bottles, ascitic fluid culture is negative in 50% of cases of spontaneous bacterial peritonitis (SBP).To determine whether 16S rDNA gene detection by real-time polymerase chain reaction (PCR) and sequencing increases the efficacy of culture in microbiological diagnosis of spontaneous bacterial peritonitis.We ...
Soriano, G +10 more
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Bacterial DNA Methylation and Methylomes
2016Formation of C5-methylcytosine, N4-methylcytosine, and N6-methyladenine in bacterial genomes is postreplicative and involves transfer of a methyl group from S-adenosyl-methionine to a base embedded in a specific DNA sequence context. Most bacterial DNA methyltransferases belong to restriction-modification systems; in addition, "solitary" or "orphan ...
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Macrophages ingest and are activated by bacterial DNA
The Journal of Immunology, 1996Abstract Recent evidence suggests that bacterial DNA activates immune responses. Here we showed that TNF-α mRNA was induced in bone marrow-derived macrophages and the macrophage cell line RAW 264 by plasmid DNA, but not by DNaseI-digested plasmid, plasmid methylated on CpG dinucleotides, or by vertebrate genomic DNA, which is ...
Stacey, K. J., Sweet, M. J., Hume, D. A.
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Separation of Choanoflagellate and Bacterial Genomic DNA
Cold Spring Harbor Protocols, 2009INTRODUCTIONChoanoflagellates are heterotrophic nanoflagellates: small, colorless protozoa that are present in marine and freshwater environments as well as in hydrated soils. Because they are the closest living relatives of the metazoa, the study of their cell biology and genomes promises to provide new insights into metazoan ancestry and origins ...
Nicole, King +4 more
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Preservation of Bacterial DNA by Human Dentin
Journal of Endodontics, 2014The capacity of dentin and collagen to bind DNA and protect against spontaneous and nuclease-induced degradation was evaluated individually and by the incubation of DNA with nuclease-producing bacteria in a mixed culture.Extracted Fusobacterium nucleatum DNA was incubated with dentin shavings or collagen for 90 minutes.
Malin, Brundin +3 more
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DNA transfer and DNA synthesis during bacterial conjugation
Zeitschrift f�r Vererbungslehre, 1966Mutant strains ofEscherichia coli, which were thermosensitive with respect to DNA replication, were used for conjugation experiments at 37°C and 42°C. Inhibition of DNA synthesis in the donor strain has no influence on the yield of recombinats. Inhibition of DNA synthesis in the recipient strain is accompanied by a complete loss of recombinant ...
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Bacterial topoisomerases and the control of DNA supercoiling
Trends in Genetics, 1990DNA in bacterial cells is under negative superhelical tension, a feature that facilitates many of the activities of DNA. Supercoiling is introduced enzymatically by DNA gyrase, and the accumulation of excessively high levels is prevented by the relaxing activity of DNA topoisomerase I.
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The Antigenicity of Bacterial DNA
2002DNA is a complex macromolecule whose immunological properties vary with base sequence and backbone structure. Although now recognized as important for normal immunity, the antigenic and immunogenic properties of DNA were originally conceptualized entirely in the context of systemic lupus erythematosus (SLE).
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