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P88 Cell free DNA bacterial sequences in patients with lupus nephritis differ from patients with IgA nephropathy and membranous nephropathy

Poster presentations, 2020
Background The circulating free DNA (cfDNA) originating mostly from the abnormal cell apoptosis, necrosis or netosis contains sequences of microorganisms encountered previously by the patients.
B. Foroncewicz   +8 more
semanticscholar   +1 more source

Bacterial DNA Methylation and Methylomes.

Advances in Experimental Medicine and Biology, 2016
Formation of C5-methylcytosine, N4-methylcytosine, and N6-methyladenine in bacterial genomes is postreplicative and involves transfer of a methyl group from S-adenosyl-methionine to a base embedded in a specific DNA sequence context. Most bacterial DNA methyltransferases belong to restriction-modification systems; in addition, "solitary" or "orphan ...
J. Casadesús
semanticscholar   +4 more sources

Preparing Bacterial Genomic DNA

Cold Spring Harbor Protocols, 2022
We describe two alternative procedures for purifying bacterial chromosomal DNA. The first procedure incorporates the use of a commercial kit based on silica membrane technology. This approach relies on the selective binding of DNA to a silica-based column in the presence of chaotropic salts (guanidine salts).
Nara Figueroa-Bossi   +2 more
openaire   +2 more sources

Preparation of Bacterial Plasmid DNA

Current Protocols in Neuroscience, 1998
AbstractThe protocols in this unit describe methods for preparing bacterial plasmid DNA free from chromosomal DNA. The first is an alkaline lysis miniprep suitable for screening a moderate number of bacterial colonies by restriction endonuclease cleavage and agarose gel electrophoresis.
JoAnne Engebrecht   +2 more
openaire   +4 more sources

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