Results 21 to 30 of about 1,269,047 (286)
Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast
BMC Microbiology, 2022 Background Sequence-based methods for the detection of bacteria such as 16S rRNA amplicon sequencing and metagenomics can provide a comprehensive view of the bacterial microbiome of food.
Annika Flint +8 more
doaj +1 more source
Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3 [PDF]
, 2012 Large-scale production of plasmid DNA to prepare therapeutic gene vectors or DNA-based vaccines requires a suitable bacterial host, which can stably maintain the plasmid DNA during industrial cultivation.
Al-Allaf, FA +4 more
core +1 more source
Comparison of five assays for DNA extraction from bacterial cells in human faecal samples [PDF]
, 2020 Aim To determine the most effective DNA extraction method for bacteria in faecal samples. Materials and Results This study assessed five commercial methods, that is, NucliSens easyMag, QIAamp DNA Stool Mini kit, PureLink Microbiome DNA purification kit ...
Glorieux, Griet +3 more
core +1 more source
High Sensitivity of Shotgun Metagenomic Sequencing in Colon Tissue Biopsy by Host DNA Depletion
Genomics, Proteomics & Bioinformatics, 2023 The high host genetic background of tissue biopsies hinders the application of shotgun metagenomic sequencing in characterizing the tissue microbiota. We proposed an optimized method that removed host DNA from colon biopsies and examined the effect on ...
Wing Yin Cheng +8 more
doaj +1 more source
How endo- is endo-?: surface sterilization of delicate samples: a Bryopsis (Bryopsidales, Chlorophyta) case study [PDF]
, 2010 In the search for endosymbiotic bacteria, elimination of ectosymbionts is a key point of attention. Commonly, the surface of the host itself or the symbiotic structures are sterilized with aggressive substances such as chlorine or mercury derivatives ...
De Clerck, Olivier +3 more
core +2 more sources
The bacterial capsule is a gatekeeper for mobile DNA
PLOS Biology, 2021 The horizontal transfer of mobile DNA is one of the signature moves of bacterial evolution, but the specific rules that govern this transfer remain elusive. In this PLOS Biology issue, Haudiquet and colleagues revealed that the interactions between mobile genetic elements and the bacterial capsule shape the horizontal flow of DNA in an important ...
Alfonso Santos-López +2 more
openaire +6 more sources
Microorganisms, 2023 The gut microbiota has gained increasing attention in recent years due to its significant impact on colorectal cancer (CRC) development and progression.
Robertina Giacconi +14 more
doaj +1 more source
The antimicrobial polymer PHMB enters cells and selectively condenses bacterial chromosomes [PDF]
, 2016 To combat infection and antimicrobial resistance, it is helpful to elucidate drug mechanism(s) of action. Here we examined how the widely used antimicrobial polyhexamethylene biguanide (PHMB) kills bacteria selectively over host cells.
A Gentile +41 more
core +3 more sources
Bacteria-human somatic cell lateral gene transfer is enriched in cancer samples. [PDF]
PLoS Computational Biology, 2013 There are 10× more bacterial cells in our bodies from the microbiome than human cells. Viral DNA is known to integrate in the human genome, but the integration of bacterial DNA has not been described. Using publicly available sequence data from the human
David R Riley +6 more
doaj +1 more source
Canadian Journal of Infectious Diseases and Medical Microbiology, 2005 Three group B Neisseria meningitidis isolates, recovered from meningococcal disease cases in Canada and typed as B:2c:P1.5, were characterized. Multilocus sequence typing showed that all three isolates were related because of an identical sequence ...
Raymond SW Tsang +5 more
doaj +1 more source