Results 21 to 30 of about 699,746 (100)

Efficiency of chemical versus mechanical disruption methods of DNA extraction for the identification of oral Gram-positive and Gram-negative bacteria

Journal of International Medical Research, 2020
Objective Clinical diagnostics often requires the detection of multiple bacterial species in limited clinical samples with a single DNA extraction method.
Xiaolan Li, Caroline J. Bosch-Tijhof, Xi Wei, Johannes J. de Soet, Wim Crielaard, Cor van Loveren, Dong Mei Deng   +6 more
doaj   +1 more source

Optimizing bacterial DNA extraction in urine.

PLoS ONE, 2019
Urine is an acceptable, non-invasive sample for investigating the human urogenital microbiota and for the diagnosis of sexually transmitted infections.
Matthew M Munch, Laura C Chambers, Lisa E Manhart, Dan Domogala, Anthony Lopez, David N Fredricks, Sujatha Srinivasan   +6 more
doaj   +1 more source

Incorporation characteristics of exogenous 15N-labeled thymidine, deoxyadenosine, deoxyguanosine and deoxycytidine into bacterial DNA.

PLoS ONE, 2020
Bacterial production has been often estimated from DNA synthesis rates by using tritium-labeled thymidine. Some bacteria species cannot incorporate extracellular thymidine into their DNA, suggesting their biomass production might be overlooked when using
Kenji Tsuchiya, Tomoharu Sano, Noriko Tomioka, Ayato Kohzu, Kazuhiro Komatsu, Ryuichiro Shinohara, Shinji Shimode, Tatsuki Toda, Akio Imai   +8 more
doaj   +1 more source

A newly identified IncY plasmid from multi-drug-resistant Escherichia coli isolated from dairy cattle feces in Poland

Microbiology Spectrum
Comprehensive whole-genome sequencing was performed on two multi-drug-resistant Escherichia coli strains isolated from cattle manure from a typical dairy farm in Poland in 2020.
Magdalena Zalewska, Aleksandra Błażejewska, Jan Gawor, Dorota Adamska, Krzysztof Goryca, Michał Szeląg, Patryk Kalinowski, Magdalena Popowska   +7 more
doaj   +1 more source

Maturation of Pseudo-Nucleus Compartment in P. aeruginosa, Infected with Giant phiKZ Phage

Viruses, 2020
The giant phiKZ phage infection induces the appearance of a pseudo-nucleus inside the bacterial cytoplasm. Here, we used RT-PCR, fluorescent in situ hybridization (FISH), electron tomography, and analytical electron microscopy to study the morphology of ...
Yana A. Danilova, Viktoriia V. Belousova, Andrey V. Moiseenko, Innokentii E. Vishnyakov, Maria V. Yakunina, Olga S. Sokolova   +5 more
doaj   +1 more source

Effects of preservation method on canine (Canis lupus familiaris) fecal microbiota [PDF]

PeerJ, 2018
Studies involving gut microbiome analysis play an increasing role in the evaluation of health and disease in humans and animals alike. Fecal sampling methods for DNA preservation in laboratory, clinical, and field settings can greatly influence ...
Katti R. Horng, Holly H. Ganz, Jonathan A. Eisen, Stanley L. Marks   +3 more
doaj   +2 more sources

Is there a placental microbiota? A critical review and re-analysis of published placental microbiota datasets

BMC Microbiology, 2023
The existence of a placental microbiota is debated. The human placenta has historically been considered sterile and microbial colonization was associated with adverse pregnancy outcomes.
Jonathan J. Panzer, Roberto Romero, Jonathan M. Greenberg, Andrew D. Winters, Jose Galaz, Nardhy Gomez-Lopez, Kevin R. Theis   +6 more
doaj   +1 more source

Effect of bacterial DNA enrichment on detection and quantification of bacteria in an infected tissue model by metagenomic next-generation sequencing

ISME Communications, 2022
Before implementing metagenomic next-generation sequencing (mNGS) in the routine diagnostic laboratory, several challenges need to be resolved. To address strengths and limitations of mNGS in bacterial detection and quantification in samples with ...
Vladimir Lazarevic, Nadia Gaïa, Myriam Girard, Florian Mauffrey, Etienne Ruppé, Jacques Schrenzel   +5 more
doaj   +1 more source

High Sensitivity of Shotgun Metagenomic Sequencing in Colon Tissue Biopsy by Host DNA Depletion

Genomics, Proteomics & Bioinformatics, 2023
The high host genetic background of tissue biopsies hinders the application of shotgun metagenomic sequencing in characterizing the tissue microbiota. We proposed an optimized method that removed host DNA from colon biopsies and examined the effect on ...
Wing Yin Cheng, Wei-Xin Liu, Yanqiang Ding, Guoping Wang, Yu Shi, Eagle S.H. Chu, Sunny Wong, Joseph J.Y. Sung, Jun Yu   +8 more
doaj  

DnaG Primase—A Target for the Development of Novel Antibacterial Agents

Antibiotics, 2018
The bacterial primase—an essential component in the replisome—is a promising but underexploited target for novel antibiotic drugs. Bacterial primases have a markedly different structure than the human primase.
Stefan Ilic, Shira Cohen, Meenakshi Singh, Benjamin Tam, Adi Dayan, Barak Akabayov   +5 more
doaj   +1 more source