Results 21 to 30 of about 4,728,729 (346)

Identification of a Contact-Dependent Growth Inhibition (CDI) System That Reduces Biofilm Formation and Host Cell Adhesion of Acinetobacter baumannii DSM30011 Strain

Frontiers in Microbiology, 2019
Acinetobacter baumannii is a multidrug-resistant nosocomial opportunistic pathogen that is becoming a major health threat worldwide. In this study, we have focused on the A.
Morgane Roussin, Sedera Rabarioelina, Laurence Cluzeau, Julien Cayron, Christian Lesterlin, Suzana P. Salcedo, Sarah Bigot, Sarah Bigot   +7 more
doaj   +1 more source

Optimization of DNA extraction from human urinary samples for mycobiome community profiling. [PDF]

, 2019
IntroductionRecent data suggest the urinary tract hosts a microbial community of varying composition, even in the absence of infection. Culture-independent methodologies, such as next-generation sequencing of conserved ribosomal DNA sequences, provide an
Ackerman, A Lenore, Ackerman, James E, Anger, Jennifer Tash, Freeman, Michael R, Khalique, Muhammad Umair, Kim, Jayoung, NIH Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP), Tang, Jie, Underhill, David M   +8 more
core   +3 more sources

Plasmids of Psychrotolerant Polaromonas spp. Isolated From Arctic and Antarctic Glaciers – Diversity and Role in Adaptation to Polar Environments

Frontiers in Microbiology, 2018
Cold-active bacteria of the genus Polaromonas (class Betaproteobacteria) are important components of glacial microbiomes. In this study, extrachromosomal replicons of 26 psychrotolerant Polaromonas strains, isolated from Arctic and Antarctic glaciers ...
Anna Ciok, Karol Budzik, Marek K. Zdanowski, Jan Gawor, Jakub Grzesiak, Przemyslaw Decewicz, Robert Gromadka, Dariusz Bartosik, Lukasz Dziewit   +8 more
doaj   +1 more source

Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3 [PDF]

, 2012
Large-scale production of plasmid DNA to prepare therapeutic gene vectors or DNA-based vaccines requires a suitable bacterial host, which can stably maintain the plasmid DNA during industrial cultivation.
Al-Allaf, FA, Mehmet, H, Tchetchelnitski, V, Tolmachov, OE, Zambetti, LP   +4 more
core   +1 more source

Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast

BMC Microbiology, 2022
Background Sequence-based methods for the detection of bacteria such as 16S rRNA amplicon sequencing and metagenomics can provide a comprehensive view of the bacterial microbiome of food.
Annika Flint, Anna Laidlaw, Leo Li, Courtney Raitt, Mary Rao, Ashley Cooper, Kelly Weedmark, Catherine Carrillo, Sandeep Tamber   +8 more
doaj   +1 more source

The antimicrobial polymer PHMB enters cells and selectively condenses bacterial chromosomes [PDF]

, 2016
To combat infection and antimicrobial resistance, it is helpful to elucidate drug mechanism(s) of action. Here we examined how the widely used antimicrobial polyhexamethylene biguanide (PHMB) kills bacteria selectively over host cells.
A Gentile, B Spellberg, CE Barry, CR Messick, DJ Dwyer, EE Creppy, EF Palermo, FW O’Grady, G Muller, G Muller, G Rusciano, GS Gordon, J Portugal, JD McCool, JM Boberek, JP Armitage, L Good, LP O’Malley, M Mondhe, MA Kohanski, MJ Allen, MJ Allen, MJ Allen, N Nekhotiaeva, Nakashima, P Broxton, P Broxton, P Broxton, P Erbacher, P Gilbert, R de Vries, R Dryselius, R Firdessa, R Spurio, RF Murphy, S Amoils, S Goh, S Wessels, T Ikeda, T Ikeda, T Kornberg, VB Teif   +41 more
core   +3 more sources

How endo- is endo-?: surface sterilization of delicate samples: a Bryopsis (Bryopsidales, Chlorophyta) case study [PDF]

, 2010
In the search for endosymbiotic bacteria, elimination of ectosymbionts is a key point of attention. Commonly, the surface of the host itself or the symbiotic structures are sterilized with aggressive substances such as chlorine or mercury derivatives ...
De Clerck, Olivier, Hollants, Joke, Leliaert, Frédérik, Willems, Anne   +3 more
core   +2 more sources

The DNA Damage Inducible SOS Response Is a Key Player in the Generation of Bacterial Persister Cells and Population Wide Tolerance

Frontiers in Microbiology, 2020
Population-wide tolerance and persisters enable susceptible bacterial cells to endure hostile environments, including antimicrobial exposure. The SOS response can play a significant role in the generation of persister cells, population-wide tolerance ...
Z. Podlesek, Darja Žgur Bertok
semanticscholar   +1 more source

High Sensitivity of Shotgun Metagenomic Sequencing in Colon Tissue Biopsy by Host DNA Depletion

Genomics, Proteomics & Bioinformatics, 2023
The high host genetic background of tissue biopsies hinders the application of shotgun metagenomic sequencing in characterizing the tissue microbiota. We proposed an optimized method that removed host DNA from colon biopsies and examined the effect on ...
Wing Yin Cheng, Wei-Xin Liu, Yanqiang Ding, Guoping Wang, Yu Shi, Eagle S.H. Chu, Sunny Wong, Joseph J.Y. Sung, Jun Yu   +8 more
doaj   +1 more source

Potential Capsule Switching from Serogroup Y to B: The Characterization of Three such Neisseria meningitidis Isolates Causing Invasive Meningococcal Disease in Canada

Canadian Journal of Infectious Diseases and Medical Microbiology, 2005
Three group B Neisseria meningitidis isolates, recovered from meningococcal disease cases in Canada and typed as B:2c:P1.5, were characterized. Multilocus sequence typing showed that all three isolates were related because of an identical sequence ...
Raymond SW Tsang, Dennis KS Law, Shaun D Tyler, Gwen S Stephens, Mark Bigham, Wendell D Zollinger   +5 more
doaj   +1 more source