Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast
Background Sequence-based methods for the detection of bacteria such as 16S rRNA amplicon sequencing and metagenomics can provide a comprehensive view of the bacterial microbiome of food.
Annika Flint +8 more
doaj +1 more source
Small variable segments constitute a major type of diversity of bacterial genomes at the species level [PDF]
Analysis of large scale diversity in bacterial genomes has mainly focused on elements such as pathogenicity islands, or more generally, genomic islands. These comprise numerous genes and confer important phenotypes, which are present or absent depending ...
El Karoui, Meriem +12 more
core +1 more source
Structural Basis of Gate-DNA Breakage and Resealing by Type II Topoisomerases [PDF]
ype II DNA topoisomerases are ubiquitous enzymes with essential functions in DNA replication, recombination and transcription. They change DNA topology by forming a transient covalent cleavage complex with a gate-DNA duplex that allows transport of a ...
Laponogov, I +27 more
core +1 more source
High Sensitivity of Shotgun Metagenomic Sequencing in Colon Tissue Biopsy by Host DNA Depletion
The high host genetic background of tissue biopsies hinders the application of shotgun metagenomic sequencing in characterizing the tissue microbiota. We proposed an optimized method that removed host DNA from colon biopsies and examined the effect on ...
Wing Yin Cheng +8 more
doaj +1 more source
Discovery of cyanophage genomes which contain mitochondrial DNA polymerase [PDF]
DNA polymerase γ is a family A DNA polymerase responsible for the replication of mitochondrial DNA in eukaryotes. The origins of DNA polymerase γ have remained elusive because it is not present in any known bacterium, though it has been hypothesized that
Whitworth, Anna L. +19 more
core +1 more source
Three group B Neisseria meningitidis isolates, recovered from meningococcal disease cases in Canada and typed as B:2c:P1.5, were characterized. Multilocus sequence typing showed that all three isolates were related because of an identical sequence ...
Raymond SW Tsang +5 more
doaj +1 more source
Identification of DNA motifs implicated in maintenance of bacterial core genomes by predictive modeling [PDF]
Bacterial biodiversity at the species level, in terms of gene acquisition or loss, is so immense that it raises the question of how essential chromosomal regions are spared from uncontrolled rearrangements.
El Karoui, Meriem +20 more
core +2 more sources
New emerging bacterial plant diseases are spreading fast in different geographical regions, and effective safety measures have not been found. Xylella fastidiosa (Xf) is a Gram-negative bacterial plant pathogen classified as a priority quarantine pest ...
W.B.S. Machini, A.M. Oliveira-Brett
doaj +1 more source
Crenarchaeal chromatin proteins Cren7 and Sul7 compact DNA by inducing rigid bends [PDF]
Archaeal chromatin proteins share molecular and functional similarities with both bacterial and eukaryotic chromatin proteins. These proteins play an important role in functionally organizing the genomic DNA into a compact nucleoid.
White, Malcolm F. +19 more
core +1 more source
Bacteria-human somatic cell lateral gene transfer is enriched in cancer samples. [PDF]
There are 10× more bacterial cells in our bodies from the microbiome than human cells. Viral DNA is known to integrate in the human genome, but the integration of bacterial DNA has not been described. Using publicly available sequence data from the human
David R Riley +6 more
doaj +1 more source

