Draft genome sequence of the naturally competent Bacillus simplex strain WY10 [PDF]
, 2017 We sequenced a naturally competent bacterial isolate, WY10, cultured from a Wyoming soil sample. Sequence analysis revealed that WY10 is a novel strain of Bacillus simplex .
Adhya, Sankar L +3 more
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An efficient strategy for broad-range detection of low abundance bacteria without DNA decontamination of PCR reagents. [PDF]
PLoS ONE, 2011 BACKGROUND: Bacterial DNA contamination in PCR reagents has been a long standing problem that hampers the adoption of broad-range PCR in clinical and applied microbiology, particularly in detection of low abundance bacteria.
Shy-Shin Chang +3 more
doaj +1 more source
The Impact of Submersion on the Quantification of Host and Bacterial DNA [PDF]
, 2020 The Impact of Submersion on the Quantification of Host and Bacterial DNA Grace Sprouse, Depts. of Forensic Science and Chemistry, with Dr. Catherine Connon, Dept. of Forensic Science and Dr. Baneshwar Singh, Dept.
Sprouse, Grace
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Maturation of Pseudo-Nucleus Compartment in P. aeruginosa, Infected with Giant phiKZ Phage
Viruses, 2020 The giant phiKZ phage infection induces the appearance of a pseudo-nucleus inside the bacterial cytoplasm. Here, we used RT-PCR, fluorescent in situ hybridization (FISH), electron tomography, and analytical electron microscopy to study the morphology of ...
Yana A. Danilova +5 more
doaj +1 more source
Substrate-specific clades of active marine methylotrophs associated with a phytoplankton bloom in a temperate coastal environment [PDF]
, 2008 Marine microorganisms that consume one-carbon (C1) compounds are poorly described, despite their impact on global climate via an influence on aquatic and atmospheric chemistry.
Boden, Rich +4 more
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Indirect DNA extraction method suitable for acidic soil with high clay content
MethodsX, 2018 DNA extraction is an essential procedure when investigating microbial communities in environmental samples by sequencing technologies. High clay soils can be problematic as DNA adsorbs to the clay particles and can thereby be preserved from lysed, non ...
Eva Högfors-Rönnholm +3 more
doaj +1 more source
Bacterial DNA amplifies neutrophilic inflammation in IL-17-exposed airways
ERJ Open Research, 2023 Background Neutrophilic asthma (NA) is associated with increased airway interleukin (IL)-17 and abnormal bacterial community such as dominance of nontypeable Haemophilus influenzae (NTHi), particularly during asthma exacerbations.
Nastaran Mues +8 more
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Dynamics of Bacteriophage Genome Ejection In Vitro and In Vivo
, 2010 Bacteriophages, phages for short, are viruses of bacteria. The majority of phages contain a double-stranded DNA genome packaged in a capsid at a density of ~500 mg/ml. This high density requires substantial compression of the normal B form helix, leading
Molineux, Ian J., Panja, Debabrata
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Structural snapshots of Xer recombination reveal activation by synaptic complex remodeling and DNA bending [PDF]
, 2016 Bacterial Xer site-specific recombinases play an essential genome maintenance role by unlinking chromosome multimers, but their mechanism of action has remained structurally uncharacterized. Here, we present two high-resolution structures of Helicobacter
Adams +71 more
core +4 more sources
Optimizing bacterial DNA extraction in urine.
PLoS ONE, 2019 Urine is an acceptable, non-invasive sample for investigating the human urogenital microbiota and for the diagnosis of sexually transmitted infections.
Matthew M Munch +6 more
doaj +1 more source