Results 51 to 60 of about 4,728,729 (346)

Efficiency of chemical versus mechanical disruption methods of DNA extraction for the identification of oral Gram-positive and Gram-negative bacteria

open access: yesJournal of International Medical Research, 2020
Objective Clinical diagnostics often requires the detection of multiple bacterial species in limited clinical samples with a single DNA extraction method.
Xiaolan Li   +6 more
doaj   +1 more source

An efficient strategy for broad-range detection of low abundance bacteria without DNA decontamination of PCR reagents. [PDF]

open access: yesPLoS ONE, 2011
BACKGROUND: Bacterial DNA contamination in PCR reagents has been a long standing problem that hampers the adoption of broad-range PCR in clinical and applied microbiology, particularly in detection of low abundance bacteria.
Shy-Shin Chang   +3 more
doaj   +1 more source

Draft genome sequence of the naturally competent Bacillus simplex strain WY10 [PDF]

open access: yes, 2017
We sequenced a naturally competent bacterial isolate, WY10, cultured from a Wyoming soil sample. Sequence analysis revealed that WY10 is a novel strain of Bacillus simplex .
Adhya, Sankar L   +3 more
core   +2 more sources

Bacterial Extracellular DNA Promotes β-Amyloid Aggregation

open access: yesMicroorganisms, 2021
Alzheimer’s disease is associated with prion-like aggregation of the amyloid β (Aβ) peptide and the subsequent accumulation of misfolded neurotoxic aggregates in the brain.
George Tetz, Victor Tetz
doaj   +1 more source

Toll Meets Bacterial CpG-DNA [PDF]

open access: yesImmunity, 2001
While this minireview was in process, Hayashi et al. reported that bacterial flagellin signals via TLR5. Hayashi, F., Smith, K.D., Ozinsky, A., Hawn, T.R., Yi, E.C., Goodlett, D.R., Eng, J.K., Akira, S., Underhill, D.M., and Aderem, A. (2001). Nature 410, 1099–1103.
openaire   +2 more sources

Multiple origins of prokaryotic and eukaryotic single-stranded DNA viruses from bacterial and archaeal plasmids

open access: yesNature Communications, 2019
Single-stranded (ss) DNA viruses are a major component of the earth virome. In particular, the circular, Rep-encoding ssDNA (CRESS-DNA) viruses show high diversity and abundance in various habitats.
Darius Kazlauskas   +3 more
semanticscholar   +1 more source

Indirect DNA extraction method suitable for acidic soil with high clay content

open access: yesMethodsX, 2018
DNA extraction is an essential procedure when investigating microbial communities in environmental samples by sequencing technologies. High clay soils can be problematic as DNA adsorbs to the clay particles and can thereby be preserved from lysed, non ...
Eva Högfors-Rönnholm   +3 more
doaj   +1 more source

Dynamics of Bacteriophage Genome Ejection In Vitro and In Vivo

open access: yes, 2010
Bacteriophages, phages for short, are viruses of bacteria. The majority of phages contain a double-stranded DNA genome packaged in a capsid at a density of ~500 mg/ml. This high density requires substantial compression of the normal B form helix, leading
Molineux, Ian J., Panja, Debabrata
core   +1 more source

The emergence and fate of horizontally acquired genes in Escherichia coli [PDF]

open access: yes, 2008
Bacterial species, and even strains within species, can vary greatly in their gene contents and metabolic capabilities. We examine the evolution of this diversity by assessing the distribution and ancestry of each gene in 13 sequenced isolates of ...
Passel, M.W.J., van   +2 more
core   +2 more sources

Reciprocal control of viral infection and phosphoinositide dynamics

open access: yesFEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

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