Results 51 to 60 of about 1,269,047 (286)
Bacterial DNA topology and infectious disease [PDF]
Nucleic Acids Research, 2008 The gram-negative bacterium Escherichia coli and its close relative Salmonella enterica have made important contributions historically to our understanding of how bacteria control DNA supercoiling and of how supercoiling influences gene expression and vice versa.
Charles J. Dorman, Colin P. Corcoran
openaire +5 more sources
Indirect DNA extraction method suitable for acidic soil with high clay content
MethodsX, 2018 DNA extraction is an essential procedure when investigating microbial communities in environmental samples by sequencing technologies. High clay soils can be problematic as DNA adsorbs to the clay particles and can thereby be preserved from lysed, non ...
Eva Högfors-Rönnholm +3 more
doaj +1 more source
Impact of Different Fecal Processing Methods on Assessments of Bacterial Diversity in the Human Intestine. [PDF]
, 2016 The intestinal microbiota are integral to understanding the relationships between nutrition and health. Therefore, fecal sampling and processing protocols for metagenomic surveys should be sufficiently robust, accurate, and reliable to identify the ...
Anne Raggio +7 more
core +3 more sources
Circulating histones as clinical biomarkers in critically ill conditions
FEBS Letters, EarlyView.Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez +17 more
wiley +1 more source
Maturation of Pseudo-Nucleus Compartment in P. aeruginosa, Infected with Giant phiKZ Phage
Viruses, 2020 The giant phiKZ phage infection induces the appearance of a pseudo-nucleus inside the bacterial cytoplasm. Here, we used RT-PCR, fluorescent in situ hybridization (FISH), electron tomography, and analytical electron microscopy to study the morphology of ...
Yana A. Danilova +5 more
doaj +1 more source
Bacterial DNA amplifies neutrophilic inflammation in IL-17-exposed airways
ERJ Open Research, 2023 Background Neutrophilic asthma (NA) is associated with increased airway interleukin (IL)-17 and abnormal bacterial community such as dominance of nontypeable Haemophilus influenzae (NTHi), particularly during asthma exacerbations.
Nastaran Mues +8 more
doaj +1 more source
Dynamics of Bacteriophage Genome Ejection In Vitro and In Vivo
, 2010 Bacteriophages, phages for short, are viruses of bacteria. The majority of phages contain a double-stranded DNA genome packaged in a capsid at a density of ~500 mg/ml. This high density requires substantial compression of the normal B form helix, leading
Molineux, Ian J., Panja, Debabrata
core +1 more source
Genome-wide identification of essential genes in the invasive Streptococcus anginosus strain
Scientific ReportsStreptococcus anginosus, part of the Streptococcus anginosus group (SAG), is a human commensal increasingly recognized as an opportunistic pathogen responsible for abscesses formation and infections, also invasive ones.
Aleksandra Kuryłek +4 more
doaj +1 more source
Microbiology SpectrumComprehensive whole-genome sequencing was performed on two multi-drug-resistant Escherichia coli strains isolated from cattle manure from a typical dairy farm in Poland in 2020.
Magdalena Zalewska +7 more
doaj +1 more source
PLoS ONE, 2020 Bacterial production has been often estimated from DNA synthesis rates by using tritium-labeled thymidine. Some bacteria species cannot incorporate extracellular thymidine into their DNA, suggesting their biomass production might be overlooked when using
Kenji Tsuchiya +8 more
doaj +1 more source